Human IL-8 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects IL-8 in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC. Cited in 3 publication(s).
Human IL-8 ELISA Kit EZ-Set™ (DIY Antibody Pairs) Info At A Glance
|Size:||For 5 plates, 96 wells each, reagents only, plates are not included|
|Sample Type:||cell culture supernates, serum and plasma(heparin, EDTA, citrate).|
|Sample Volume:||100μl per well|
|Assay Range:||15.6pg/ml-1000pg/ml(cell culture supernates)7.8pg/ml-500pg/ml(serum,plasma)|
|Product Name||Human IL-8 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
See all CXCL8/IL-8 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects IL-8 in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.|
|Cite This Product||Human IL-8 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0413)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, serum and plasma(heparin, EDTA, citrate).|
|Assay Range||15.6pg/ml-1000pg/ml(cell culture supernates)7.8pg/ml-500pg/ml(serum,plasma)|
|Immunogen/Standard||Expression system for standard: E.coli; Immunogen sequence: S28-S99|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||For 5 plates, 96 wells each, reagents only, plates are not included|
Gene/Protein Basic Information For CXCL8 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||3576|
|Species Of This Entry||Human|
|Superfamily||intercrine alpha (chemokine CxC) family|
|Alternative Names||CXCL8/IL-8|3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; LUCT; LYNAP; MDNCF; MDNCFb-ENAP; member 8; MONAP; MONAPGCP1; NAF; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1|
|Post Tranlational Modifications||Phosphorylation; Acetylation; Methylation; Cleavage; Oxidation; Reduction; Nitration; Glycosylation; Carboxylation; Citrullination; Ubiquitination; Sulphation; Demethylation; Nitrosylation; Dephosphorylation; Biotinylation; Deacetylation; Myristoylation|
|Gene Location||4q13.3, on Chromasome 4, gene sequence: NC_000004.12 (73740569..73743716)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CXCL8 (Source: Uniprot.org, NCBI)
|Protein Function||IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.|
|Interacting Proteins||CXCR2; PF4; TNFAIP6|
|Research Areas||Angiogenesis; Cancer; Chemokines and Cytokines; Cytokine Research; Immunology; Innate Immunity; Signal Transduction|
|Related Diseases||Inflammation; Malignant Neoplasms; Inflammatory Response; Neoplasms; Tissue Adhesions; Carcinoma; Tumor Angiogenesis; Lung Diseases; Infective Disorder; Fibrosis; Asthma; Atherosclerosis; Chronic Obstructive Airway Disease; Cell Invasion; Neoplasm Metastasis; Arthritis; Pneumonia; Pathologic Neovascularization; Innate Immune Response; Lung Diseases, Obstructive|
|Related Pathways||Secretion; Pathogenesis; Inflammatory Response; Immune Response; Angiogenesis; Cytokine Production; Cell Proliferation; Chemotaxis; Innate Immune Response; Cell Activation; Cell Adhesion; Cytokine Secretion; Cell Growth; Cell Death; Cell Migration; Virulence; Localization; Cell Cycle; Phagocytosis; Wound Healing|
|Background||Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor(MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils. IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications. The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members(platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside. Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.|
|Scientific References||PMID: 2953813 by Schmid J., et al. Induction of mRNA for a serine protease and a beta-thromboglobulin- like protein in mitogen-stimulated human leukocytes.|
PMID: 3260265 by Matsushima K., et al. Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction of MDNCF mRNA by interleukin 1 and tumor necrosis factor.
Kit Components And Assay QC Data Details Of Human IL-8 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EZ0413-CAP||96-well plate precoated with anti-Human CXCL8/IL-8 antibody||1|
|EZ0413-ST||lyophilized recombinant Human CXCL8/IL-8 standard||10ng/tube|
|EZ0413-DA||biotinylated anti-Human CXCL8/IL-8 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EZ0413 Human IL-8 ELISA Kit EZ-Set™ (DIY Antibody Pairs). It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human IL-8 ELISA Kit EZ-Set™ (DIY Antibody Pairs) within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
We measured the performance consistency of Human IL-8 ELISA Kit EZ-Set™ (DIY Antibody Pairs) to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 3
|il-8 regulates the stemness properties of cancer stem cells in the small-cell lung cancer cell line h446||PubMed ID 30254465|
|The conditioned medium of human mesenchymal stromal cells reduces irradiation-induced damage in cardiac fibroblast cells||PubMed ID 30010837|
|The Effects of Resveratrol on Inflammation and Oxidative Stress in a Rat Model of Chronic Obstructive Pulmonary Disease||PubMed ID 28895883|
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