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SKU:EK0413
Application:ELISA
Sensitivity:<1pg/ml
Sample Type:cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Price: $250.00
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Overview

Product Name Human IL-8 PicoKine™ ELISA Kit
Description For quantitative detection of human IL-8 in cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Cite This Product Human IL-8 PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0413)
Validated Species Human
Application ELISA

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Immunogen Expression system for standard: E.coli; Immunogen sequence: S28-S99
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Pack Size 96wells/kit

Properties

Sensitivity <1pg/ml
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
Assay Range 15.6pg/ml-1000pg/ml(cell culture supernates)7.8pg/ml-500pg/ml(serum,plasma)
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
Sample Type cell culture supernates, serum and plasma(heparin, EDTA, citrate).

*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine™ ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
Capture Antibody monoclonal antibody from mouse
Detection Antibody polyclonal antibody from goat
Natural Levels Some research articles suggesting the natural levels of CXCL8 are listed below:
Storage Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Kit Components

Catalog numberDescriptionQuantity
EK0413-CAP96-well plate precoated with anti-Human CXCL8 antibody1
EK0413-STlyophilized recombinant Human CXCL8 standard10ng/tubex2
EK0413-DAbiotinylated anti-Human CXCL8 antibody130ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)130ul(dilution 1:100)
AR1106-1sample diluent buffer30ml
AR1106-2antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
AR0030-EPBS washing bufferPowder for 1000ml

Washing buffer Preparation: Disolve AR0030-E to 1000ml distilled water and adjust pH to 7.2~7.6. Finally, adjust the total volume to 1L.

*Additional components can be purchased. If you need extra of the above components please order them together to avoid additional shipping charges.

See Prices For Extra Components

Material Required But Not Provided

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
*Reagents needed for sample collection and preparation are not included in the kit.

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name CXCL8
Protein Name Interleukin-8
Molecular Weight 11098 MW
Protein Function IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. .
Sequence Similarities Belongs to the intercrine alpha (chemokine CxC) family.
Subcellular Localization Secreted.
Uniprot ID P10145
Alternative Names Interleukin-8;IL-8;C-X-C motif chemokine 8;Chemokine (C-X-C motif) ligand 8;Emoctakin;Granulocyte chemotactic protein 1;GCP-1;Monocyte-derived neutrophil chemotactic factor;MDNCF;Monocyte-derived neutrophil-activating peptide;MONAP;Neutrophil-activating protein 1;NAP-1;Protein 3-10C;T-cell chemotactic factor;MDNCF-a;GCP/IL-8 protein IV;IL8/NAP1 form I;Interleukin-8;(Ala-IL-8)77;GCP/IL-8 protein II;IL-8(1-77);IL8/NAP1 form II;MDNCF-b;IL-8(5-77);IL-8(6-77);(Ser-IL-8)72;GCP/IL-8 protein I;IL8/NAP1 form III;Lymphocyte-derived neutrophil-activating factor;LYNAP;MDNCF-c;Neutrophil-activating factor;NAF;IL-8(7-77);GCP/IL-8 protein V;IL8/NAP1 form IV;IL-8(8-77);GCP/IL-8 protein VI;IL8/NAP1 form V;IL-8(9-77);GCP/IL-8 protein III;IL8/NAP1 form VI;CXCL8;IL8;
Research Areas CXCL8|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Interleukin-8

Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor(MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils. IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications. The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members(platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside. Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.

Human IL-8 PicoKine™ ELISA Kit Images

Click the images to enlarge.

Human IL-8 PicoKine™ ELISA Kit
Human IL-8 PicoKine ELISA Kit standard curve

Intra/Inter Assay Precision

>
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n161616242424
Mean(pg/ml)115357616176392649
Standard deviation6.6717.541.313.3821.1745.43
CV(%)5.84.96.77.65.47

Typical Data Obtained from Human IL-8 PicoKine™ ELISA Kit

(TMB reaction incubate at 37°C for 15-20min)

Concentration(pg/ml)015.631.262.51252505001000
O.D.0.0030.1700.3210.5941.1211.8782.5522.779

*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.

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Publications

Li J, Yang L, Yao Q, Li Y, Tian Y, Li S, Jiang S, Wang Y, Li X, Guo Z. Evid Based Complement Alternat Med. 2014;2014:381976. Doi: 10.1155/2014/381976. Epub 2014 May 14. Effects And Mechanism Of Bufei Yishen Formula In A Rat Chronic Obstructive Pul...
Dai Jp, Zhao Xf, Zeng J, Wan Qy, Yang Jc, Li Wz, Chen Xx, Wang Gf, Li Ks. Plos One. 2013 Apr 16;8(4):E61026. Doi: 10.1371/Journal.Pone.0061026. Print 2013. Drug Screening For Autophagy Inhibitors Based On The Dissociation Of Beclin1-Bcl2 Complex U...
Xie Xj, Ye Yf, Zhou L, Xie Hy, Jiang Gp, Feng Xw, He Y, Xie Qf, Zheng Ss. J Zhejiang Univ Sci B. 2010 Nov;11(11):819-27. Doi: 10.1631/Jzus.B1000030. Th17 Promotes Acute Rejection Following Liver Transplantation In Rats.
Dong Sj, Zhong Yq, Lu Wt, Li Gh, Jiang Hl, Mao B. Inflammation. 2015 Aug;38(4):1493-501. Doi: 10.1007/S10753-015-0124-2. Baicalin Inhibits Lipopolysaccharide-Induced Inflammation Through Signaling Nf-??b Pathway In Hbe16 Airway Epithelial Cells.
Yu Q, Wang Z, Yang Q. J Dairy Sci. 2012 Oct;95(10):5580-7. Doi: 10.3168/Jds.2012-5540. Epub 2012 Aug 9. Lactobacillus Amylophilus D14 Protects Tight Junction From Enteropathogenic Bacteria Damage In Caco-2 Cells.
Li Ff, Shen J, Shen Hj, Zhang X, Cao R, Zhang Y, Qui Q, Lin Xx, Xie Yc, Zhang Lh, Jia Yl, Dong Xw, Jiang Jx, Bao Mj, Zhang S, Ma Wj, Wu Xm, Shen H, Xie Qm, Ke Y. J Immunol. 2012 Sep 15;189(6):3159-67. Doi: 10.4049/Jimmunol.1200197. Epub 2012 Aug 1...
He L, Zhang E, Shi J, Li X, Zhou K, Zhang Q, Le Ad, Tang X. Cancer Chemother Pharmacol. 2013 Mar;71(3):713-25. Doi: 10.1007/S00280-012-2063-Z. Epub 2013 Jan 6. (-)-Epigallocatechin-3-Gallate Inhibits Human Papillomavirus (Hpv)-16 Oncoprotein-Induc...
Bao Mh, Zhang Yw, Lou Xy, Xiao Y, Cheng Y, Zhou Hh. Can J Physiol Pharmacol. 2014 Apr;92(4):299-306. Doi: 10.1139/Cjpp-2013-0322. Epub 2014 Jan 10. Puerarin Protects Endothelial Cells From Oxidized Low Density Lipoprotein Induced Injuries Via The ...
Zhai X, Han Q, Shan Z, Qu X, Guo L, Zhou Y. Mol Med Rep. 2014 Dec;10(6):3052-8. Doi: 10.3892/Mmr.2014.2575. Epub 2014 Sep 18. Dual Specificity Phosphatase 6 Suppresses The Growth And Metastasis Of Prostate Cancer Cells.
Wang H, Zhuo Y, Hu X, Shen W, Zhang Y, Chu T. Biochem Biophys Res Commun. 2015 Mar 6;458(2):268-73. Doi: 10.1016/J.Bbrc.2015.01.097. Epub 2015 Feb 3. Cd147 Deficiency Blocks Il-8 Secretion And Inhibits Lung Cancer-Induced Osteoclastogenesis.
Pei X, Li M, Zhan J, Yu Y, Wei X, Guan L, Aydin H, Elson P, Zhou M, He H, Zhang H. Plos One. 2015 Apr 28;10(4):E0124338. Doi: 10.1371/Journal.Pone.0124338. Ecollection 2015. Enhanced Imp3 Expression Activates Nf-??b Pathway And Promotes Renal Cell ...
Xiao X, Gang Y, Wang H, Wang J, Zhao L, Xu L, Liu Z. Biochem Biophys Res Commun. 2015 Feb 6;457(2):221-6. Doi: 10.1016/J.Bbrc.2014.12.091. Epub 2014 Dec 27. Double-Stranded Rna Transcribed From Vector-Based Oligodeoxynucleotide Acts As Transcripti...
Li X, Qian D, Ju F, Wang B. Oncol Lett. 2015 Jan;9(1):365-370. Epub 2014 Oct 15. Upregulation Of Toll-Like Receptor 2 Expression In Colorectal Cancer Infected By Human Cytomegalovirus.
Zhang E, Feng X, Liu F, Zhang P, Liang J, Tang X. Plos One. 2014 Jul 24;9(7):E103440. Doi: 10.1371/Journal.Pone.0103440. Ecollection 2014. Roles Of Pi3K/Akt And C-Jun Signaling Pathways In Human Papillomavirus Type 16 Oncoprotein-Induced Hif-1??, V...
Chen L, Xiao Z, Meng Y, Zhao Y, Han J, Su G, Chen B, Dai J. Biomaterials. 2012 Feb;33(5):1437-44. Doi: 10.1016/J.Biomaterials.2011.10.056. Epub 2011 Nov 10. The Enhancement Of Cancer Stem Cell Properties Of Mcf-7 Cells In 3D Collagen Scaffolds For...
Liu Lj, Zhang J, Xiao Zf, Dai B, Sun My, Chen L, Chen B. J Biomed Mater Res B Appl Biomater. 2014 May;102(4):772-80. Doi: 10.1002/Jbm.B.33058. Epub 2013 Oct 21. Three-Dimensional Collagen Scaffold Enhances The Human Adenoid Cystic Carcinoma Cancer...
Ping C, Yongping Z, Minmin Q, Weiyan Y, Yaozong Y. Dig Dis Sci. 2010 Jun;55(6):1599-609. Doi: 10.1007/S10620-009-0909-Y. Epub 2009 Aug 13. Activated Protein C Improves The Severity Of Severe Acute Pancreatitis Via Up-Regulating The Expressions Of ...
Zhang H, Yin G, Jiang H, Zhang C. J Int Med Res. 2013 Aug;41(4):1179-86. Doi: 10.1177/0300060513488503. Epub 2013 Jun 14. High-Dose N-Acetylcysteine Decreases Silica-Induced Lung Fibrosis In The Rat.
Li Mc, Yang G, Zhou Xd, Tselluyko S, Perelman Jm. Int J Mol Med. 2014 Jan;33(1):83-90. Doi: 10.3892/Ijmm.2013.1535. Epub 2013 Oct 23. The Pathophysiological Mechanisms Underlying Mucus Hypersecretion Induced By Cold Temperatures In Cigarette Smoke...
Ren Dl, Li Yj, Hu Bb, Wang H, Hu B. J Pineal Res. 2015 May;58(4):452-60. Doi: 10.1111/Jpi.12230. Epub 2015 Mar 26. Melatonin Regulates The Rhythmic Migration Of Neutrophils In Live Zebrafish.
Liu L, Li C, Xiang J, Dong W, Cao Z. J Periodontal Res. 2013 Oct;48(5):615-22. Doi: 10.1111/Jre.12047. Epub 2013 Feb 27. Over-Expression And Potential Role Of Cyclophilin A In Human Periodontitis.
Capsoni F, Ongari Am, Lonati C, Accetta R, Gatti S, Catania A. Bmc Musculoskelet Disord. 2015 Jun 21;16:154. Doi: 10.1186/S12891-015-0615-1. ??-Melanocyte-Stimulating-Hormone (??-Msh) Modulates Human Chondrocyte Activation Induced By Proinflammatory...
Li P, Yu Q, Ye X, Wang Z, Yang Q. Microbiology. 2011 Sep;157(Pt 9):2639-46. Doi: 10.1099/Mic.0.049148-0. Epub 2011 Jun 23. Lactobacillus S-Layer Protein Inhibition Of Salmonella-Induced Reorganization Of The Cytoskeleton And Activation Of Mapk Sig...
Ma Yj, He M, Han Ja, Yang L, Ji Xy. Scand J Immunol. 2013 Oct;78(4):387-93. Doi: 10.1111/Sji.12097. A Clinical Study Of Hbsag-Activated Dendritic Cells And Cytokine-Induced Killer Cells During The Treatment For Chronic Hepatitis B.
Li Y, Li Sy, Li Js, Deng L, Tian Yg, Jiang Sl, Wang Y, Wang Yy. Biol Pharm Bull. 2012;35(10):1752-60. Epub 2012 Aug 3. A Rat Model For Stable Chronic Obstructive Pulmonary Disease Induced By Cigarette Smoke Inhalation And Repetitive Bacterial Infe...
Li G, He L, Zhang E, Shi J, Zhang Q, Le Ad, Zhou K, Tang X. Cancer Lett. 2011 Dec 8;311(2):160-70. Doi: 10.1016/J.Canlet.2011.07.012. Epub 2011 Jul 22. Overexpression Of Human Papillomavirus (Hpv) Type 16 Oncoproteins Promotes Angiogenesis Via Enh...
Hu Mm, Yang Q, Zhang J, Liu Sm, Zhang Y, Lin H, Huang Zf, Wang Yy, Zhang Xd, Zhong B, Shu Hb. Proc Natl Acad Sci U S A. 2014 Jan 28;111(4):1509-14. Doi: 10.1073/Pnas.1318227111. Epub 2014 Jan 13. Trim38 Inhibits Tnf??- And Il-1??-Triggered Nf-??b Act...
Yang C, Ling H, Zhang M, Yang Z, Wang X, Zeng F, Wang C, Feng J. Mol Cells. 2011 Jun;31(6):531-8. Doi: 10.1007/S10059-011-1025-3. Epub 2011 Apr 20. Oxidative Stress Mediates Chemical Hypoxia-Induced Injury And Inflammation By Activating Nf-??b-Cox-...
Hu Y, Chen X, Duan H, Hu Y, Mu X. Immunopharmacol Immunotoxicol. 2009;31(4):550-5. Doi: 10.3109/08923970902814129. Chinese Herbal Medicinal Ingredients Inhibit Secretion Of Il-6, Il-8, E-Selectin And Txb2 In Lps-Induced Rat Intestinal Microvascula...
Yang J, Yu Hm, Zhou Xd, Kolosov Vp, Perelman Jm. Mol Immunol. 2013 Jan;53(1-2):161-71. Doi: 10.1016/J.Molimm.2012.06.015. Epub 2012 Aug 30. Study On Trpv1-Mediated Mechanism For The Hypersecretion Of Mucus In Respiratory Inflammation.
Li Q, Zhou X, Kolosov Vp, Perelman Jm. Cell Biochem Biophys. 2012 Apr;62(3):421-31. Doi: 10.1007/S12013-011-9324-Z. The Expression And Pharmacological Characterization Of Nicotinic Acetylcholine Receptor Subunits In Hbe16 Airway Epithelial Cells.
Yu J, Lu Y, Li Y, Xiao L, Xing Y, Li Y, Wu L. J Pharm Pharmacol. 2015 Sep;67(9):1240-50. Doi: 10.1111/Jphp.12415. Epub 2015 Apr 16. Role Of S100A1 In Hypoxia-Induced Inflammatory Response In Cardiomyocytes Via Tlr4/Ros/Nf-??b Pathway.
Kong J, Kong L, Kong J, Ke S, Gao J, Ding X, Zheng L, Sun H, Sun W. J Transl Med. 2012 Nov 21;10:230. Doi: 10.1186/1479-5876-10-230. After Insufficient Radiofrequency Ablation, Tumor-Associated Endothelial Cells Exhibit Enhanced Angiogenesis And P...
Zhang H, Xia X, Han F, Jiang Q, Rong Y, Song D, Wang Y. Mol Pharm. 2015 May 4;12(5):1648-61. Doi: 10.1021/Acs.Molpharmaceut.5B00069. Epub 2015 Apr 2. Cathelicidin-Bf, A Novel Antimicrobial Peptide From Bungarus Fasciatus, Attenuates Disease In A D...
Yu Zm, Liu Zh, Chen J, Zeng Q. Chin J Integr Med. 2014 Dec;20(12):934-43. Doi: 10.1007/S11655-014-1863-X. Epub 2014 Nov 27. Anti-Inflammatory Effect Of Qingwen Baidu Decoction (????????????
Liu W, Shan Lp, Dong Xs, Liu Xw, Ma T, Liu Z. World J Gastroenterol. 2013 Jan 28;19(4):492-502. Doi: 10.3748/Wjg.V19.I4.492. Combined Early Fluid Resuscitation And Hydrogen Inhalation Attenuates Lung And Intestine Injury.

FAQs

Q: The protocol says to use neutral PBS and provides a recipe. Could I use neutral PBS made with KCl or KH2PO4 (common constituents for most PBS recipes) instead? Keywords: protocol, alternative buffer, applications, reagent recipe
A: Yes, it is ok to use common PBS recipes. We have tried many types of buffers with common constituents, and no significant difference was observed whatsoever.
Q: Why are my O.D. values different than your values on the datasheet? Keyword: troubleshooting, reference
A: We detected the kit in our lab and got our values on the datasheet before delivery, but protein activity will decrease as time goes on, so you may get lower O.D. values than ours. However, it should still be in a reasonable range and the standards can be used to calculate sample values. Good linearity of curve is more important than the actual numerical value.
Q: On the ELISA kit datasheet it says "HRP substrate TMB was used to visualize HRP enzymatic reaction." What is HRP and which part of the kit contains HRP? Keyword: kit components, reagents, protocol
A: It means Avidin-Biotin-Peroxidase Complex(AR1103) and you could find it in Kit Components.
Q: Do you offer any ELISA kits that can work with whole blood samples instead of plasma or urine? Keyword: applications
A: We test serum and plasma routinely, and there is very little difference between serum, plasma and whole blood. The whole blood also contains proteins we need to test. The kit can be used to test whole blood in theory.
Q: Have you tested this kit with pig samples? Keyword: applications, porcine, detection
A: No, we have not tested this with pig samples. A pilot experiment is recommended first if you plan to detect pig samples.
Q: Does silicic acid (formula SiOH4) affect the results of the ELISA assay? Keyword: protocol, reagents
A: The acid may affect the binding of antigen to antibody, it is not recommended to use. That being said, it is unlikely to affect the reaction if the solution remains an overall neutral environment.
Q: Is there lot-to-lot variation of the ELISA kits? What are Boster's general services when I have questions about your kits? Keyword: technical support, help, customer service
A: The NIBSC/WHO 1st International Standard is evaluated, we always test our kits before delivery, and customers can find the test result on the protocol. If the customer needs technical support from us to analyze their data, please contact us and we ask that you provide us the following information: the lot# and production date, and when did the customer detect the kit.
Q: The results of my standards do not look correct, what could be the problem? Keyword: troubleshoot, protocol
A: Double check the protocol for plate washing. It should be examined on three aspects: 1) Did you make the washing buffer correctly? 2) How long did you let the buffer stay in wells? 3) What was the volume of the washing buffer added to each well? In addition, pay attention when adding sample to avoid contamination. And we suggest testing the standards again. Values of standards at low concentration are more affected than that at higher concentration, so customer can still get expected values at high concentration even if there is an error. If problems persist, please contact technical support with the catalog and lot number of your product.
Q: What is the well depth of the 96 well plates for the ELISA kits? Keyword: well capacity, product size
A: The well depth is 300ul, and the max capacity is 350ul. The height of the well is 1.1 cm, and the internal size is 1 cm.
Q: The kit does not include wash solution, what should I do? Keyword: wash buffer
A: Our Elisa kits do not come with wash solution, but we have included information about how to make washing buffer on the datasheet. Please refer to the "Material Required But Not Provided" section. We also offer washing buffer for sale separately (Phosphate Buffered Saline Powder SKU: AR0030).
Q: For your ELISA kits, are the capture and detection antibodies polyclonals or monoclonals? Keyword: antibody clonality
A: This information can be found for each kit under the "Properties" section, and you can find the immunogen sequence information in the "Overview" section.
Q: Do your ELISA kits come with sealants or plate covers? Keyword: storage, sequential use
A: The kit can be used within a month sequentially if it's opened and stored at 4 degree. There is no need to use sealants, the plate can be packaged with aluminum foil bag, and for other reagents keep bottle tightly closed.
Q: What is the concentration of Sodium Azide in your Elisa kits? Keyword: preservative
A: Our Elisa kits contain 0.02% Sodium Azide. All of the components except TMB colour developing reagent and stop solution contains 0.02% Sodium azide as the preservative.
Q: Are there positive and negative controls available for my ELISA kit? Keyword: positive control, negative control
A: We can provide a recombinant protein as a control. It costs $50 per control and takes 2 weeks to manufacture. We cannot provide a positive or negative control in serum.
Q: Why do I get positive results for my knockout (KO) model when used as a control?
A: The knockout (KO) model may contain truncated forms of the target protein which can be detected by the ELISA assay.
Q: How long do I soak my plate in the wash buffer?
A: The plate should soak in 0.3mL PBS or TBS wash buffer for at least 1-2 minutes in an automated wash station.
Q: Can I use Tween in my wash buffer?
A: While it is not recommended to use Tween in your wash buffer, small amounts (<0.1% concentration) may decrease background due to insufficient blocking.
Q: What plate type do I use to set up the microplate reader?
A: Our plates are made with the Corning costar plates similar to the DNA-BIND 96 -well plates.
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the normal level of this protein in my sample of interest?
A: We have reviewed literature and have gathered this information for most of our ELISA kits. You can find this information on the product page or contact us if you cannot find it. However this information is only suggestive and cannot replace pilot studies in determining the optimal sample dilution ratio.
Q: Is the plate separable? Can I use only part of the kit and save the rest for later? How many samples can I run with one kit?
A: Yes the plate is separable. There are 12 strips of 8 wells each, all removable from the plate. The amount of samples you can run depend on a few factors. In the most common ELISA set up, you will use two strips for standards, and 10 strips for samples using duplicates, which let you run up to 40 samples per kit. Contact us if you have questions regarding other situations.
Q: Does the kit contain sample preparation reagents? How do I prepare my samples?
A: Since different sample types require different reagents, we do not include them in the ELISA kit. However we do have each reagent mentioned in the file below available at very reasonable prices. Be sure to check them out. For sample preparation protocols please download the file below: https://www.bosterbio.com/media/pdf/Boster_Sample_Preparation_Protocols.pdf
Q: Can this ELISA kit work on brain tissue homogenate, cell culture supernatant, saliva, urine, serum, whole blood or any other sample type?
A: In theory the ELISA kit will work for all sample types if the target protein is present at a level that falls within the linear range of the ELISA kit detection range. We guarantee the kit to work on the sample types that we have tested. If you want dilution ratio suggestions on these sample types please contact our technical support. For sample types that we have not tested for, we suggest you run pilot experiments to decide the optimal sample dilution.
Q: Can this ELISA kit react with the pro-form of the target protein? Can this ELISA kit react with an isoform of the protein?
A: In general, unless otherwise specified, the ELISA kit is pan-specific, meaning that it will react with all different forms of the target protein if they share the majority of the target protein's sequence. The capture and detection antibodies are reactive to the entire sequence of the standard protein. You can find the sequence information of the standard protein in the "immunogen" section. For more information about the specificity of the kit for your particular experiment, please contact our techincal support.
Q: Can this ELISA kit react with human, mouse, rat or other species?
A: If the kit is reactive to another commonly used species (human mouse and or rat) we would have listed it as a separate product. If you want to check cross reactivity to a species that is not included in the 3 species listed above, please contact our technical support for more information. As a rule of thumb, if the sequences are 90%+ indentical, there is a high chance of cross reactivity for your species of interest.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to human il 8 elisa kit, human il-8 elisa kit, human il8 elisa kit, human interleukin 8 elisa kit