Product Info Summary
SKU: | EK1349 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants and serum. |
Product info
Product Name
Human JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1349
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human F11R in cell culture supernatants and serum. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1349)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: S28-V238
Sensitivity
<10 pg/ml
Assay Range
46.9 pg/ml - 3,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1349 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1349 is reactive to F11r in Human samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK1349 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of JAM-A
Junctional adhesion molecule A (JAM-A) is a protein that in humans is encoded by the F11R gene. It is mapped to 1q23.3. This gene is an immunoglobulin-like molecule that colocalizes with tight junctions in endothelium and epithelium and is also found on blood leukocytes and platelets. JAM-A plays an important role in the regulation of tight junction assembly in epithelia. In addition, it can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration, and a platelet receptor. JAM-A has a nonredundant role in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK1349-CAP | Anti-Human F11r Pre-coated 96-well strip microplate | 1 |
EK1349-ST | Human F11r Standard | 2 vials, 10 ng/tube |
EK1349-DA | Human F11r Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 |
O.D. | 0.011 | 0.122 | 0.187 | 0.314 | 0.615 | 0.923 | 1.435 | 1.858 |
Data Example Images
Click image to see more details
Human JAM-A PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect JAM-A, Dilution ratio of 1:1, concentration in serum is 280-330 pg/ml..
Some articles we found to cite concentrations of JAM-A in samples: 19214165 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 81 | 325 | 1280 | 73 | 310 | 1209 |
Standard deviation | 5.83 | 16.9 | 67.84 | 5.32 | 16.43 | 93.09 |
CV (%) | 7.2% | 5.2% | 5.3% | 7.3% | 5.3% | 7.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of JAM-A in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 81 | 88 | 85 | 93 | 86 | 4.38 | 5% |
Sample 2 | 325 | 306 | 319 | 326 | 319 | 7.96 | 2.4% |
Sample 3 | 1280 | 1301 | 1409 | 1335 | 1331 | 48.99 | 3.6% |
Protein Target Info & Infographic
Gene/Protein Information For F11r (Source: Uniprot.Org, NCBI)
Gene Name
F11r
Full Name
Junctional adhesion molecule A
Weight
32.424kDa
Superfamily
immunoglobulin superfamily
Alternative Names
CD321 antigen; CD321; F11 receptor; F11R; JAM-1; JAM1CD321; JAMA; JAM-A; JCAMJAM; Junctional adhesion molecule 1Platelet F11 receptor; junctional adhesion molecule A; PAM-1; PAM-1KAT; Platelet adhesion molecule 1 F11r|9130004G24, AA638916, ESTM3, ESTM33, JAM, JAM-, JAM-1, JAM-A, Jcam, Jcam1, Ly10, Ly106|F11 receptor|junctional adhesion molecule A|BV11 antigen|F11 receptor/F11R|junction cell adhesion molecule A|junction cell adhesion molecule1|junctional adhesion molecule 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on F11r, check out the F11r Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for F11r: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine® (EK1349)
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No publications found for EK1349
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
7 Customer Q&As for Human JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine®
Question
Q: how to analyze ELISA data? I have performed JAM-A level in serum.
P. Surve
Verified customer
Asked: 2020-11-05
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-11-05
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement JAM-A using the EK1349 Human JAM-A Picokine® ELISA Kit?
D. Liu
Verified customer
Asked: 2020-07-04
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2020-07-04
Question
Q: how can I thaw whole blood sample for JAM-A ELISA after freezing?
K. Dayal
Verified customer
Asked: 2020-05-25
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test JAM-A for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-05-25
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-03-30
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-01-23
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-01-23
Question
Q: What is the optimal O.D. value for JAM-A ELISA kit? I used your JAM-A ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain JAM-A even though the O.D. values are not very high?
S. Zhou
Verified customer
Asked: 2018-02-04
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this JAM-A ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of JAM-A that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-02-04
Question
Could you let me know if EK1349 will work with human brain tissue homogenates (homogenates are in a 1% NP-40 lysis buffer)?
Verified Customer
Verified customer
Asked: 2013-01-22
Answer
Our lab hasn't woked on human brain tissue samples using EK1349. Endogenous biotin in tissue homogenates might introduce false positive signal. We recommend the customer to run a pilot test as described below to confirm the affect of endogeous biotin first. Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, the customer can work on the tissue sample by using the kit.
Boster Scientific Support
Answered: 2013-01-22