Human PCSK9 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Proprotein Convertase 9/PCSK9 ELISA antibody pairs, Human, for 5 plates

Human PCSK9 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human PCSK9 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Info Summary

SKU: EZ1147
Size: For 5 plates, 96 wells each
Reactive Species: Human
Application: ELISA

Product Name

Human PCSK9 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all Proprotein Convertase 9/PCSK9 products

SKU/Catalog Number

EZ1147

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Human PCSK9 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human PCSK9 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human PCSK9 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ1147)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: S153-Q692

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ1147 is reactive to Pcsk9 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).

Application Guarantee

EZ1147 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Storage of opened/reconstituted material
EZ1147-CA Rabbit anti- human PCSK9 polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ1147-DA Biotinylated goat anti- human PCSK9 polyclonal antibody (Detection Antibody) 500 µl, 28 µg/mL (recommended dilution 1:100)
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)
EK1147-ST Lyophilized recombinant human PCSK9 standard 10 ng/tube×3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Incubator.
  • 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 5. Clean tubes and Eppendorf tubes.
  • 6. 96 well microplate (Cat# AR1100)
  • 7. Plate Sealers.
  • 8. Capture Antibody Diluent: PBS.
  • 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 12. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0580.1120.140.2350.3910.731.3142.215

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2291991540421519275944
Standard deviation16.03125.43243.1817.63132.96285.31
CV (%)7%6.3%4.5%8.2%6.9%4.8%

Gene/Protein Information For Pcsk9 (Source: Uniprot.Org, NCBI)

Gene Name

Pcsk9

Full Name

Proprotein convertase subtilisin/kexin type 9

Weight

74.823kDa

Superfamily

peptidase S8 family

Alternative Names

EC 3.4.21; EC 3.4.21.111; FH3; FH3neural apoptosis regulated convertase 1; FHCL3; HCHOLA3; hypercholesterolemia, autosomal dominant 3; LDLCQ1; NARC1; NARC-1; NARC-1convertase subtilisin/kexin type 9 preproprotein; NARC1EC 3.4.21.-; Neural apoptosis-regulated convertase 1; PC9; PCSK9; Proprotein Convertase 9; proprotein convertase subtilisin/kexin type 9; Subtilisin/kexin-like protease PC9 Pcsk9|AI415265, AI747682, FH3, HCHOLA3, Na, Narc1, PC9|proprotein convertase subtilisin/kexin type 9|proprotein convertase subtilisin/kexin type 9|NARC-1|convertase subtilisin|neural apoptosis regulated convertase 1|proprotein convertase 9|proprotein convertase PC9|subtilisin/kexin-like protease PC9

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Pcsk9, check out the Pcsk9 Infographic

Pcsk9 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Pcsk9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

No publications found for EZ1147

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Human PCSK9 ELISA Kit EZ-Set™ (DIY Antibody Pairs)?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Human PCSK9 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for Human PCSK9 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

$500
Backordered.

Lead time for this item is typically 2-3 weeks

Get A Quote
Eddy test
In stock
Order Product
EZ1147
$500.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.