Human PRDX2/Peroxiredoxin-2 ELISA Kit PicoKine®

Peroxiredoxin 2 ELISA kit for Human

Human Peroxiredoxin 2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PRDX2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK1528
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human PRDX2/Peroxiredoxin-2 ELISA Kit PicoKine®

View all Peroxiredoxin 2 ELISA kits

SKU/Catalog Number

EK1528

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Peroxiredoxin 2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PRDX2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human PRDX2/Peroxiredoxin-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1528)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A2-N198

Sensitivity

<10 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1528 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1528 is reactive to PRDX2 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1528 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Peroxiredoxin 2

Peroxiredoxin-2 is a protein that in humans is encoded by the PRDX2 gene. This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein plays an antioxidant protective role in cells, and it may contribute to the antiviral activity of CD8 (+) T-cells. The crystal structure of this protein has been resolved to 2.7 angstroms. This protein prevents hemolytic anemia from oxidative stress by stabilizing hemoglobin, thus making this gene a therapeutic target for patients with hemolytic anemia. It may have a proliferative effect and play a role in cancer development or progression. Related pseudogenes have been identified on chromosomes 5, 6, 10 and 13.

Kit Components

Catalog Number Description Quantity
EK1528-CAP Anti-Human PRDX2 Pre-coated 96-well strip microplate 1
EK1528-ST Human PRDX2 Standard 2 vials, 20 ng/tube
EK1528-DA Human PRDX2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03126251250250050001000020000
O.D.0.0510.1850.2680.4040.7551.1201.7202.330

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Peroxiredoxin 2, Dilution ratio of 1:5, concentration in serum and plasma is around 13 ng/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human PRDX2/Peroxiredoxin-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)40531224453444534169285
Standard deviation22.27140.49638.7727.14198.12724.23
CV (%)5.5%4.5%6.7%6.1%5.8%7.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Peroxiredoxin 2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 140541641946242521.75.1%
Sample 23122301431402995306763.922%
Sample 3953489571027695869588467.554.8%
*number of samples for each test n=16.

Gene/Protein Information For PRDX2 (Source: Uniprot.Org, NCBI)

Gene Name

PRDX2

Full Name

Peroxiredoxin-2

Weight

21.892kDa

Superfamily

peroxiredoxin family

Alternative Names

EC 1.11.1; MGC4104; Natural killer cell-enhancing factor B; natural killer-enhancing factor B; NKEF-B; NKEFBNKEF-B; Peroxiredoxin 2; peroxiredoxin-2; PRDX2; PRPTDPX1; PRX2; PRXII; TDPX1; thiol-specific antioxidant 1; Thiol-specific antioxidant protein; Thioredoxin peroxidase 1; Thioredoxin-dependent peroxide reductase 1; torin; TPX1; TSA; TSAEC 1.11.1.15 PRDX2 HEL-S-2a, NKEF-B, NKEFB, PRP, PRX2, PRXII, PTX1, TDPX1, TPX1, TSA peroxiredoxin 2 peroxiredoxin-2|epididymis secretory sperm binding protein Li 2a|natural killer cell-enhancing factor B|thiol-specific antioxidant 1|thioredoxin peroxidase 1|thioredoxin-dependent peroxide reductase 1|thioredoxin-dependent peroxiredoxin 2|torin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PRDX2, check out the PRDX2 Infographic

PRDX2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PRDX2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK1528 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Yang,J.C.-S.;Huang,L.-H.;Wu,S.-C.;Kuo,P.-J.;Wu,Y.-C.;Wu,C.-J.;Lin,C.-W.;Tsai,P.-Y.;Hsieh,C.-H.Lymphaticovenous Anastomosis Supermicrosurgery Decreases Oxidative Stress and Increases Antioxidant Capacity in the Serum of Lymphedema Patients.J.Clin.Med.2021,10,1540.https://doi.org/10.3390/jcm10071540
Species: Human
EK1528 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

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7 Customer Q&As for Human PRDX2/Peroxiredoxin-2 ELISA Kit PicoKine®

Question

Q: how to thaw whole blood sample for Peroxiredoxin 2 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-04-06

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Peroxiredoxin 2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-04-06

Question

Q: if the enzyme conjugated Peroxiredoxin 2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Peroxiredoxin 2 antigen?

K. Turner

Verified customer

Asked: 2019-11-05

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-11-05

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Peroxiredoxin 2 using the EK1528 Human Peroxiredoxin 2 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-10-26

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2018-10-26

Question

Q: how to proceed with the analysis of ELISA data? I have performed Peroxiredoxin 2 level in serum.

Verified Customer

Verified customer

Asked: 2018-05-07

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-05-07

Question

Will EK1528 react with Prx1, Prx3, Prx4, Prx5 and Prx6 proteins?

Verified Customer

Verified customer

Asked: 2017-08-08

Answer

EK1528 will react with Prx1, Prx3, Prx4, Prx5 proteins.<br> Please see the following detected cross reactivity. <br> human recombinant Prx1, 0.2% <br>human recombinant Prx3, 5.1% <br>human recombinant Prx4, 19.8% <br>human recombinant Prx5, 0.3% <br>We do not have Prx6 protein for testing. It is uncertain if EK1528 will cross react with Prx6 protein.

Boster Scientific Support

Answered: 2017-08-08

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2014-04-28

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2014-04-28

Question

Q: What is the optimal O.D. value for Peroxiredoxin 2 ELISA kit? I used your Peroxiredoxin 2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Peroxiredoxin 2 even though the O.D. values are not very high?

A. Nelson

Verified customer

Asked: 2014-04-19

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Peroxiredoxin 2 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Peroxiredoxin 2 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2014-04-19

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