Human PLA2G7/Lp-PLA2 ELISA Kit PicoKine®

PLA2G7/PAF-AH/Lp-PLA2 ELISA kit for Human

Human PLA2G7/Lp-PLA2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PLA2G7 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.

Product Info Summary

SKU: EK1637
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Human PLA2G7/Lp-PLA2 ELISA Kit PicoKine®

View all PLA2G7/PAF-AH/Lp-PLA2 ELISA kits

SKU/Catalog Number

EK1637

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human PLA2G7/Lp-PLA2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PLA2G7 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human PLA2G7/Lp-PLA2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1637)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: F22-N441

Sensitivity

<20 pg/ml

Assay Range

0.78 ng/ml - 50 ng/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1637 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1637 is reactive to PLA2G7 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1637 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of PLA2G7/PAF-AH/Lp-PLA2

Lipoprotein-associated phospholipase A2 (Lp-PLA2) also known as platelet-activating factor acetylhydrolase (PAF-AH) is a phospholipase A2 enzyme that in humans is encoded by the PLA2G7 gene. Lp-PLA2 is a 45-kDa protein of 441 amino acids. It is one of several PAF acetylhydrolases. The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.

Kit Components

Catalog Number Description Quantity
EK1637-CAP Anti-Human PLA2G7 Pre-coated 96-well strip microplate 1
EK1637-ST Human PLA2G7 Standard 2 vials, 50 ng/tube
EK1637-DA Human PLA2G7 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)00.781.563.126.2512.52550
O.D.0.0070.1220.1840.3090.5290.8811.4552.069

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect PLA2G7/PAF-AH/Lp-PLA2, Dilution ratio of 1:10, concentration in serum and plasma is 16-200 ng/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human PLA2G7/Lp-PLA2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)1179760722123951239692022984
Standard deviation55.41418.381124.7975.57456.721539.92
CV (%)4.7%5.5%4.9%6.1%6.6%6.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of PLA2G7/PAF-AH/Lp-PLA2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11179133412121128121375.866.2%
Sample 276077415751170637399205.522.7%
Sample 322955229702118824226228341081.674.7%
*number of samples for each test n=16.

Gene/Protein Information For PLA2G7 (Source: Uniprot.Org, NCBI)

Gene Name

PLA2G7

Full Name

Platelet-activating factor acetylhydrolase

Weight

50.077kDa

Superfamily

AB hydrolase superfamily

Alternative Names

2-acetyl-1-alkylglycerophosphocholine esterase; EC 3.1.1; EC 3.1.1.47,1-alkyl-2-acetylglycerophosphocholine esterase; Group-VIIA phospholipase A2; gVIIA-PLA2; LDL-associated phospholipase A2; LDL-PLA(2); LDL-PLA2; lipoprotein-associated phospholipase A2; LpPLA2; Lp-PLA2; PAF acetylhydrolase; PAF-AH; PAFAHPAF 2-acylhydrolase; phospholipase A2, group VII (platelet-activating factor acetylhydrolase; PLA2G7; plasma); platelet-activating factor acetylhydrolase PLA2G7 LDL-PLA2, LP-PLA2, PAFAD, PAFAH phospholipase A2 group VII platelet-activating factor acetylhydrolase|1-alkyl-2-acetylglycerophosphocholine esterase|2-acetyl-1-alkylglycerophosphocholine esterase|LDL-PLA(2)|LDL-associated phospholipase A2|PAF 2-acylhydrolase|PAF acetylhydrolase|gVIIA-PLA2|group-VIIA phospholipase A2|lipoprotein-associated phospholipase A2|phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PLA2G7, check out the PLA2G7 Infographic

PLA2G7 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PLA2G7: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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7 Customer Q&As for Human PLA2G7/Lp-PLA2 ELISA Kit PicoKine®

Question

Q: if the enzyme conjugated PLA2G7 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the PLA2G7 antigen?

Z. Garcia

Verified customer

Asked: 2020-03-04

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-03-04

Question

Q: What is the optimal O.D. value for PLA2G7 ELISA kit? I performed your PLA2G7 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain PLA2G7 even though the O.D. values are not very high?

H. Smith

Verified customer

Asked: 2019-05-06

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this PLA2G7 ELISA kit to have sensitivity of 20pg/ml, that means the minimum amount of PLA2G7 that can be declared/interpreted as positive by the above standard is 20pg/ml.

Boster Scientific Support

Answered: 2019-05-06

Question

Q: which procedure should I follow in order to thaw whole blood sample for PLA2G7 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-10-28

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test PLA2G7 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2018-10-28

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-06-28

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-06-28

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-04-09

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-04-09

Question

Q: how do I analyze ELISA data? I have obtained PLA2G7 level in plasma.

W. Harris

Verified customer

Asked: 2016-09-06

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2016-09-06

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of PLA2G7 in Human plasma? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2014-12-03

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the PLA2G7 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2014-12-03

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