Human Rantes ELISA Kit PicoKine®

CCL5/RANTES ELISA kit for Human

Human Rantes ELISA Kit PicoKine™ (96 Tests). Quantitate Human CCL5 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 3pg/ml. Cited in 10 publication(s).

Product Info Summary

SKU: EK0494
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Product Name

Human Rantes ELISA Kit PicoKine®

View all CCL5/RANTES ELISA kits

SKU/Catalog Number

EK0494

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Rantes ELISA Kit PicoKine™ (96 Tests). Quantitate Human CCL5 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 3pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Rantes ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0494)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S24-S91

Sensitivity

<3 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0494 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0494 is reactive to CCL5 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Application Guarantee

EK0494 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CCL5/RANTES

Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4 (+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.

Kit Components

Catalog Number Description Quantity
EK0494-CAP Anti-Human CCL5 Pre-coated 96-well strip microplate 1
EK0494-ST Human CCL5 Standard 2 vials, 10 ng/tube
EK0494-DA Human CCL5 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0070.0510.0990.2070.4500.9341.7692.497

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect CCL5/RANTES, Dilution ratio of 1:100, concentration in serum is 6.8-17.8 ng/ml..

Some articles we found to cite concentrations of CCL5/RANTES in samples: 17504241 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Rantes ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3516644231169476
Standard deviation1.7111.4522.541.5812.6733.32
CV (%)4.9%6.9%7%%7%%7.5%7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CCL5/RANTES in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 135353938361.784.9%
Sample 21661491571691607.854.9%
Sample 344237739041840625.176.1%
*number of samples for each test n=16.

Gene/Protein Information For CCL5 (Source: Uniprot.Org, NCBI)

Gene Name

CCL5

Full Name

C-C motif chemokine 5

Weight

9.99kDa

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES CCL5 D17S136E, RANTES, SCYA5, SIS-delta, SISd, TCP228, eoCP C-C motif chemokine ligand 5 C-C motif chemokine 5|T-cell specific protein p288|beta-chemokine RANTES|chemokine (C-C motif) ligand 5|eosinophil chemotactic cytokine|regulated upon activation, normally T-expressed, and presumably secreted|small inducible cytokine subfamily A (Cys-Cys), member 5

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CCL5, check out the CCL5 Infographic

CCL5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CCL5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK0494 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

In vitro modeling of COPD inflammation and limitation of p38 inhibitor – SB203580

Mesenchymal stem cells in inflammatory microenvironment potently promote metastatic growth of cholangiocarcinoma via activating Akt/NF-κB signaling by paracrine CCL5

Combination of Gluten-Digesting Enzymes Improved Symptoms of Non-Celiac Gluten Sensitivity: A Randomized Single-blind, Placebo-controlled Crossover Study

“Dialogue” between Caco-2 and DCs regulated by Ganoderma atrum polysaccharide in intestinal-like Caco-2/DCs co-culture model

Inflammation-Associated Cytokines IGFBP1 and RANTES Impair the Megakaryocytic Potential of HSCs in PT Patients after Allo-HSCT

Yudan Wang, Xiaomeng Ding, Yi Chen, Jianhua Xie, Bing Zheng, Xinxin Chang, Shiqi Liu, Xiaobo Hu, Qiang Yu,“Dialogue” between Caco-2 and DCs regulated by Ganoderma atrum polysaccharide in intestinal-like Caco-2/DCs co-culture model,Food Research International,2021,110310,ISSN 0963-9969,https://doi.org/10.1016/j.foodres.2021.110310.
Species: Human,Mouse
EK0494 usage in article: APP:ELISA, SAMPLE:CACO2 CELL, DILUTION:NA

Chen X,Gao A,Zhang F,Yang Z,Wang S,Fang Y,Li J,Wang J,Shi W,Wang L, Zheng Y,Sun Y.ILT4 inhibition prevents TAM- and dysfunctional T cell-mediated immunosuppression and enhances the efficacy of anti-PD-L1 therapy in NSCLC with EGFR activation.Theranostics.
Species: Human,Mouse
EK0494 usage in article: APP:ELISA, SAMPLE:TUMOR CELLS, DILUTION:NA

Mesenchymal stem cells in inflammatory microenvironment potently promote metastatic growth of cholangiocarcinoma via activating Akt/NF-?B signaling by paracrine CCL5

Tick-borne encephalitis virus induces chemokine RANTES expression via activation of IRF-3 pathway

Zhao C, Bu X, Wang W, Ma T, Ma H. Plos One. 2014 Jan 8;9(1):E85058. Doi: 10.1371/Journal.Pone.0085058. Ecollection 2014. Gec-Derived Sfrp5 Inhibits Wnt5A-Induced Macrophage Chemotaxis And Activation.

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9 Customer Q&As for Human Rantes ELISA Kit PicoKine®

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CCL5 in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

B. Evans

Verified customer

Asked: 2020-10-21

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CCL5 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-10-21

Question

Q: can I use EDTA plasma as samples in Human CCL5 Picokine® ELISA Kit (Catalog # EK0494)?

W. Krishna

Verified customer

Asked: 2019-12-23

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of CCL5 causing degradation of its protein structure. CCL5 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CCL5 ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-12-23

Question

Q: if the enzyme conjugated CCL5 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CCL5 antigen?

Verified Customer

Verified customer

Asked: 2019-10-17

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-10-17

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-10-10

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-10-10

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

T. Martin

Verified customer

Asked: 2019-06-26

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-06-26

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2018-10-14

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-10-14

Question

Q: What is the optimal O.D. value for CCL5 ELISA kit? I used your CCL5 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CCL5 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-09-12

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CCL5 ELISA kit to have sensitivity of 3pg/ml, that means the minimum amount of CCL5 that can be declared/interpreted as positive by the above standard is 3pg/ml.

Boster Scientific Support

Answered: 2018-09-12

Question

Q: which procedure should I follow in order to thaw whole blood sample for CCL5 ELISA after freezing?

S. Wang

Verified customer

Asked: 2016-05-28

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CCL5 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2016-05-28

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2015-10-04

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2015-10-04

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