Human Serpin C1/Antithrombin-III PicoKine® Quick ELISA Kit

Serpin C1/Antithrombin-III ELISA kit, 120-minute protocol

Human Serpin C1/Antithrombin-III PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human SERPINC1 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml.

Product Info Summary

SKU: FEK1393
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.
Assay Time: 120 Minutes

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human Serpin C1/Antithrombin-III PicoKine® Quick ELISA Kit

See all Serpin C1/Antithrombin-III products

SKU/Catalog Number

FEK1393

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Serpin C1/Antithrombin-III PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human SERPINC1 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Serpin C1/Antithrombin-III PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1393)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: H33-K464

Sensitivity

<10 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK1393 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1393 is reactive to SERPINC1 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Application Guarantee

FEK1393 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Serpin C1/Antithrombin-III

Antithrombin III is the most important inhibitor of thrombin and other coagulation proteinases. It belongs to the serine proteinase inhibitor (serpin) superfamily of inhibitors and structurally related proteins, which contain reactive centers that have evolved to attract and entrap certain proteinases. Inherited antithrombin III deficiency (AT3D) is a risk factor for the early development of venous thromboembolism (THPH7). Antithrombin III regulates clot formation both by inhibiting thrombin activity directly and by interfering with earlier stages of the clotting cascade. By in situ hybridization and quantitative analysis of DNA dosage in carriers of chromosome 1 deletions, AT3 is mapped to 1q23-q25. The AT3 gene has 7 exons. It contains 9 complete and 1 partial repetitive ALU sequence elements, which occur in the introns of the gene at a higher frequency (about 22% of the intron sequence) than in the genome as a whole (about 5%).

Kit Components

Catalog number Description Quantity
FEK1393-CAP 96-well plate precoated with anti-Human Serpin C1/Antithrombin-III antibody 1
EK1393-ST lyophilized recombinant Human Serpin C1/Antithrombin-III standard 20 ng/tube
FEK1393-DA Biotinylated anti-Human Serpin C1/Antithrombin-III detection antibody (50x) 100 ul
- Avidin-Biotin-Peroxidase Complex (30x) 400 µl
AR1106-1 Sample diluent 30 ml
AR1106-2 Antibody diluent 12 ml
- Avidin-Biotin-Peroxidase Diluent 12 ml
AR1106-5 Wash Buffer (25x) 20 ml
AR1104 Color Developing Reagent (TMB) 10 ml
AR1105 Stop Solution 10 ml
PLA-SEA Plate Sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03126251,2502,5005,00010,00020,000
O.D.0.0110.0620.0820.150.2950.5821.3331.974

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)6471973898264720509365
Standard deviation28.46153.89377.2434.29174.25552.53
CV (%)4.4%7.8%4.2%5.3%8.5%5.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Serpin C1/Antithrombin-III in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 164764967363665113.492%
Sample 219731798168118271819103.995.7%
Sample 389828247896693778893407.684.5%
*number of samples for each test n=16.

Gene/Protein Information For SERPINC1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P01008

Gene ID

462

Gene Name

SERPINC1

Full Name

Antithrombin-III

Weight

52.602kDa

Superfamily

serpin family

Alternative Names

Antithrombin-III; AT3antithrombin-III; ATIII; ATIIIantithrombin III; MGC22579; serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1; serine-cysteine proteinase inhibitor clade C member 1; Serpin C1; serpin peptidase inhibitor, clade C (antithrombin), member 1 SERPINC1 AT3, AT3D, ATIII, ATIII-R2, ATIII-T1, ATIII-T2, THPH7 serpin family C member 1 antithrombin-III|serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1|serpin peptidase inhibitor clade C member 1|serpin peptidase inhibitor, clade C (antithrombin), member 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SERPINC1, check out the SERPINC1 Infographic

SERPINC1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for SERPINC1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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1 Customer Q&As for Human Serpin C1/Antithrombin-III PicoKine® Quick ELISA Kit

Question

Just a question; for product FEK1393 in the Western Blot for antithrombin III; is that from cells or supernatant? If it is in the cell, which cells were used? Is there any blocking protein secretion used?

Verified Customer

Verified customer

Asked: 2016-09-19

Answer

The product FEK1393 have been tested successfully in cell lysates, which do not need to have protein secretion blocked. Additionally, there were enough non-secreted Antithrombin III still in the cytoplasm of the cells to give positive bands.

Boster Scientific Support

Answered: 2016-09-19

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