Product Info Summary
SKU: | FEK1393 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. |
Assay Time: | 120 Minutes |
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Product info
Introduction
Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
Product Name
Human Serpin C1/Antithrombin-III PicoKine® Quick ELISA Kit
See all Serpin C1/Antithrombin-III products
SKU/Catalog Number
FEK1393
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Serpin C1/Antithrombin-III PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human SERPINC1 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Serpin C1/Antithrombin-III PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1393)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: H33-K464
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK1393 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1393 is reactive to SERPINC1 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.
Application Guarantee
FEK1393 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Serpin C1/Antithrombin-III
Antithrombin III is the most important inhibitor of thrombin and other coagulation proteinases. It belongs to the serine proteinase inhibitor (serpin) superfamily of inhibitors and structurally related proteins, which contain reactive centers that have evolved to attract and entrap certain proteinases. Inherited antithrombin III deficiency (AT3D) is a risk factor for the early development of venous thromboembolism (THPH7). Antithrombin III regulates clot formation both by inhibiting thrombin activity directly and by interfering with earlier stages of the clotting cascade. By in situ hybridization and quantitative analysis of DNA dosage in carriers of chromosome 1 deletions, AT3 is mapped to 1q23-q25. The AT3 gene has 7 exons. It contains 9 complete and 1 partial repetitive ALU sequence elements, which occur in the introns of the gene at a higher frequency (about 22% of the intron sequence) than in the genome as a whole (about 5%).
Kit Components
Catalog number | Description | Quantity |
---|---|---|
FEK1393-CAP | 96-well plate precoated with anti-Human Serpin C1/Antithrombin-III antibody | 1 |
EK1393-ST | lyophilized recombinant Human Serpin C1/Antithrombin-III standard | 20 ng/tube |
FEK1393-DA | Biotinylated anti-Human Serpin C1/Antithrombin-III detection antibody (50x) | 100 ul |
- | Avidin-Biotin-Peroxidase Complex (30x) | 400 µl |
AR1106-1 | Sample diluent | 30 ml |
AR1106-2 | Antibody diluent | 12 ml |
- | Avidin-Biotin-Peroxidase Diluent | 12 ml |
AR1106-5 | Wash Buffer (25x) | 20 ml |
AR1104 | Color Developing Reagent (TMB) | 10 ml |
AR1105 | Stop Solution | 10 ml |
PLA-SEA | Plate Sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 | 20,000 |
O.D. | 0.011 | 0.062 | 0.082 | 0.15 | 0.295 | 0.582 | 1.333 | 1.974 |
Data Example Images
Click image to see more details
Human Serpin C1/Antithrombin-III PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 647 | 1973 | 8982 | 647 | 2050 | 9365 |
Standard deviation | 28.46 | 153.89 | 377.24 | 34.29 | 174.25 | 552.53 |
CV (%) | 4.4% | 7.8% | 4.2% | 5.3% | 8.5% | 5.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Serpin C1/Antithrombin-III in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 647 | 649 | 673 | 636 | 651 | 13.49 | 2% |
Sample 2 | 1973 | 1798 | 1681 | 1827 | 1819 | 103.99 | 5.7% |
Sample 3 | 8982 | 8247 | 8966 | 9377 | 8893 | 407.68 | 4.5% |
Protein Target Info & Infographic
Gene/Protein Information For SERPINC1 (Source: Uniprot.Org, NCBI)
Uniprot ID
P01008
Gene ID
462
Gene Name
SERPINC1
Full Name
Antithrombin-III
Weight
52.602kDa
Superfamily
serpin family
Alternative Names
Antithrombin-III; AT3antithrombin-III; ATIII; ATIIIantithrombin III; MGC22579; serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1; serine-cysteine proteinase inhibitor clade C member 1; Serpin C1; serpin peptidase inhibitor, clade C (antithrombin), member 1 SERPINC1 AT3, AT3D, ATIII, ATIII-R2, ATIII-T1, ATIII-T2, THPH7 serpin family C member 1 antithrombin-III|serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1|serpin peptidase inhibitor clade C member 1|serpin peptidase inhibitor, clade C (antithrombin), member 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SERPINC1, check out the SERPINC1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SERPINC1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Serpin C1/Antithrombin-III PicoKine® Quick ELISA Kit (FEK1393)
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Customer Q&As
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1 Customer Q&As for Human Serpin C1/Antithrombin-III PicoKine® Quick ELISA Kit
Question
Just a question; for product FEK1393 in the Western Blot for antithrombin III; is that from cells or supernatant? If it is in the cell, which cells were used? Is there any blocking protein secretion used?
Verified Customer
Verified customer
Asked: 2016-09-19
Answer
The product FEK1393 have been tested successfully in cell lysates, which do not need to have protein secretion blocked. Additionally, there were enough non-secreted Antithrombin III still in the cytoplasm of the cells to give positive bands.
Boster Scientific Support
Answered: 2016-09-19