Human SHBG / Sex Hormone Binding Globulin ELISA Kit PicoKine®

SHBG ELISA kit for Human

Human SHBG / Sex Hormone Binding Globulin ELISA Kit PicoKine™ (96 Tests). Quantitate Human SHBG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 0.001 nmol/L.

Product Info Summary

SKU: EK1236
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human SHBG / Sex Hormone Binding Globulin ELISA Kit PicoKine®

View all SHBG ELISA kits

SKU/Catalog Number

EK1236

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human SHBG / Sex Hormone Binding Globulin ELISA Kit PicoKine™ (96 Tests). Quantitate Human SHBG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 0.001 nmol/L.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human SHBG / Sex Hormone Binding Globulin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1236)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L30-H402

Sensitivity

<0.001 nmol/L

Assay Range

0.031 nmol/L - 2 nmol/L

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1236 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1236 is reactive to SHBG in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1236 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of SHBG

Sex hormone-binding globulin (SHBG) or sex steroid-binding globulin (SSBG) is a glycoprotein that binds to the two sex hormones: androgen and estrogen. The gene for SHBG is located on chromosome 17 on the short arm between the bands 17p12→p13. And this gene encodes a steroid binding protein that was first described as a plasma protein secreted by the liver but is now thought to participate in the regulation of steroid responses. The encoded protein transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers. Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus. Alternative splicing results in multiple transcript variants.

Kit Components

Catalog Number Description Quantity
EK1236-CAP Anti-Human SHBG Pre-coated 96-well strip microplate 1
EK1236-ST Human SHBG Standard 2 vials, 0.002 nmol/tube
EK1236-DA Human SHBG Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (nmol/L)00.0310.0620.1250.250.512
O.D.0.0750.2450.3520.5240.8961.2561.8022.263

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect SHBG, For serum, dilution ratio is 1:1000 and concentration is around 130nmol/l.
For plasma, dilution ratio is 1:10 and concentration is around 2nmol/l..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human SHBG / Sex Hormone Binding Globulin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (nmol/L)6030194561312954
Standard deviation318.6660.483.6622.4667.73
CV (%)5%6.2%6.4%6%7.2%7.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of SHBG in ELISA kits from four different production batches/lots.

LotsLot 1 (nmol/L)Lot 2 (nmol/L)Lot 3 (nmol/L)Lot 4 (nmol/L)Mean (nmol/L)Standard DeviationCV (%)
Sample 160626464621.652.6%
Sample 230126029529428716.15.6%
Sample 394591086191690830.193.3%
*number of samples for each test n=16.

Gene/Protein Information For SHBG (Source: Uniprot.Org, NCBI)

Gene Name

SHBG

Full Name

Sex hormone-binding globulin

Weight

43.779kDa

Alternative Names

ABP; ABPTestosterone-estrogen-binding globulin; androgen binding protein; MGC126834; MGC138391; SBP; sex hormone-binding globulin; Sex steroid-binding protein; SHBG; TEBG; Testis-specific androgen-binding protein; testosterone-binding beta-globulin; Testosterone-estradiol-binding globulin SHBG ABP, SBP, TEBG sex hormone binding globulin sex hormone-binding globulin|sex steroid-binding protein|testis-specific androgen-binding protein|testosterone-binding beta-globulin|testosterone-estradiol-binding globulin|testosterone-estrogen-binding globulin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SHBG, check out the SHBG Infographic

SHBG infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for SHBG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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9 Customer Q&As for Human SHBG / Sex Hormone Binding Globulin ELISA Kit PicoKine®

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement SHBG using the EK1236 Human SHBG Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-09-25

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2020-09-25

Question

Q: What is the optimal O.D. value for SHBG ELISA kit? I used your SHBG ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain SHBG even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2020-07-29

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this SHBG ELISA kit to have sensitivity of 0.001nmol/L, that means the minimum amount of SHBG that can be declared/interpreted as positive by the above standard is 0.001nmol/L.

Boster Scientific Support

Answered: 2020-07-29

Question

Q: how to proceed with the analysis of ELISA data? I have obtained SHBG level in serum.

Verified Customer

Verified customer

Asked: 2019-07-09

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-07-09

Question

Q: can I use citrate plasma as samples in Human SHBG Picokine® ELISA Kit (Catalog # EK1236)?

Verified Customer

Verified customer

Asked: 2019-02-24

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of SHBG causing disruption of its protein structure. SHBG may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the SHBG ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-02-24

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

C. Green

Verified customer

Asked: 2019-01-15

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-01-15

Question

Q: how to thaw whole blood sample for SHBG ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-11-03

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test SHBG for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2018-11-03

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2015-08-06

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2015-08-06

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2014-07-27

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2014-07-27

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of SHBG in Human serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2014-07-12

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the SHBG ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2014-07-12

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