|Sample Type:||cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).|
Data & Images
|Product Name||Human Survivin PicoKine™ ELISA Kit|
|Description||Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Survivin|
|Cite This Product||Human Survivin PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0641)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Immunogen||Expression system for standard: E.coli; Immunogen sequence: M1-D142|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
|Sample Type||cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine™ ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
|Capture Antibody||monoclonal antibody from mouse|
|Detection Antibody||polyclonal antibody from goat|
|Natural Levels||Some research articles suggesting the natural levels of BIRC5 are listed below:|
|Storage||Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|-||96-well plate precoated with anti- human Survivin antibody||1|
|ST0000-4||lyophilized recombinant human Survivin standard||4ng/tubex2|
|AR1107||biotinylated anti- human Survivin antibody||130ul(dilution 1:100)|
|AR1103||Avidin-Biotin-Peroxidase Complex(ABC)||130ul(dilution 1:100)|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
*Additional components can be purchased. If you need extra of the above components please order them together to avoid additional shipping charges.See Prices For Extra Components
Material Required But Not Provided
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 4. Clean tubes and Eppendorf tubes.
- 5. Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Baculoviral IAP repeat-containing protein 5|
|Gene Full Name||Baculoviral IAP repeat-containing protein 5|
|Protein Function||Multitasking protein that has dual roles in promotingcell proliferation and preventing apoptosis. Component of achromosome passage protein complex (CPC) which is essential forchromosome alignment and segregation during mitosis andcytokinesis. Acts as an important regulator of the localization ofthis complex; directs CPC movement to different locations from theinner centromere during prometaphase to midbody during cytokinesisand participates in the organization of the center spindle byassociating with polymerized microtubules. The complex with RANplays a role in mitotic spindle formation by serving as a physicalscaffold to help deliver the RAN effector molecule TPX2 tomicrotubules. May counteract a default induction of apoptosis inG2/M phase. The acetylated form represses STAT3 transactivation oftarget gene promoters. May play a role in neoplasia. Inhibitor ofCASP3 and CASP7. Isoform 2 and isoform 3 do not appear to playvital roles in mitosis. Isoform 3 shows a marked reduction in itsanti-apoptotic effects when compared with the displayed wild-typeisoform..|
|Tissue Specificity||Expressed only in fetal kidney and liver, andto lesser extent, lung and brain. Abundantly expressed inadenocarcinoma (lung, pancreas, colon, breast, and prostate) andin high-grade lymphomas. Also expressed in various renal cellcarcinoma cell lines..|
|Sequence Similarities||Belongs to the IAP family.|
|Subcellular Localization||Cytoplasm. Nucleus. Chromosome. Chromosome,centromere. Cytoplasm, cytoskeleton, spindle. Chromosome,centromere, kinetochore. Midbody. Localizes on chromosomearms and inner centromeres from prophase through metaphase.Localizes to kinetochores in metaphase, distributes to the midzonemicrotubules in anaphase and at telophase, localizes exclusivelyto the midbody. Colocalizes with AURKB at mitotic chromosomes.Acetylation at Lys-129 directs its localization to the nucleus byenhancing homodimerization and thereby inhibiting XPO1/CRM1-mediated nuclear export.|
|Uniprot ID||BIRC5: O15392
|Alternative Names||Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivin; BIRC5; API4, IAP4;|
|Research Areas||cell biology|apoptosis|intracellular|survivin / iaps|epigenetics and nuclear signaling|chromosome structure|chromosome|scaffold proteins|cell cycle|nuclear|transcription|transcription factors|cell division cell division antibodies|cancer|invasion/microenvironment|kits/ lysates/ other|kits|elisa kits|apoptosis marker and proteins elisa kits|neuroscience target elisa kits|cell death||
Background for Baculoviral IAP repeat-containing protein 5
Human Survivin PicoKine™ ELISA Kit Images
Click the images to enlarge.
Intra/Inter Assay Precision
|Intra-Assay Precision||Inter-Assay Precision|
Typical Data Obtained from Human Survivin PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 25-30min)
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.