|Sample Type:||cell culture supernates, serum and plasma(EDTA).|
Data & Images
|Product Name||Human sVEGFR1/sFLT1 PicoKine™ ELISA Kit|
|Description||Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human sVEGFR1/sFLT1|
|Cite This Product||Human sVEGFR1/sFLT1 PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0543)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Immunogen||Expression system for standard: sf21; Immunogen sequence: S27-H687|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
|Sample Type||cell culture supernates, serum and plasma(EDTA).
*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine™ ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
|Capture Antibody||monoclonal antibody from mouse|
|Detection Antibody||polyclonal antibody from goat|
|Natural Levels||Some research articles suggesting the natural levels of FLT1 are listed below:|
|Storage||Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|-||96-well plate precoated with anti- human sVEGFR1/sFLT1 antibody||1|
|ST0000-10||lyophilized recombinant human sVEGFR1/sFLT1 standard||10ng/tubex2|
|AR1107||biotinylated anti- human sVEGFR1/sFLT1 antibody||130ul(dilution 1:100)|
|AR1103||Avidin-Biotin-Peroxidase Complex(ABC)||130ul(dilution 1:100)|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
*Additional components can be purchased. If you need extra of the above components please order them together to avoid additional shipping charges.See Prices For Extra Components
Material Required But Not Provided
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 4. Clean tubes and Eppendorf tubes.
- 5. Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Vascular endothelial growth factor receptor 1|
|Gene Full Name||Vascular endothelial growth factor receptor 1|
|Protein Function||Tyrosine-protein kinase that acts as a cell-surfacereceptor for VEGFA, VEGFB and PGF, and plays an essential role inthe development of embryonic vasculature, the regulation ofangiogenesis, cell survival, cell migration, macrophage function,chemotaxis, and cancer cell invasion. May play an essential roleas a negative regulator of embryonic angiogenesis by inhibitingexcessive proliferation of endothelial cells. Can promoteendothelial cell proliferation, survival and angiogenesis inadulthood. Its function in promoting cell proliferation seems tobe cell-type specific. Promotes PGF-mediated proliferation ofendothelial cells, proliferation of some types of cancer cells,but does not promote proliferation of normal fibroblasts (invitro). Has very high affinity for VEGFA and relatively lowprotein kinase activity; may function as a negative regulator ofVEGFA signaling by limiting the amount of free VEGFA andpreventing its binding to KDR. Likewise, isoforms lacking atransmembrane domain, such as isoform 2, isoform 3 and isoform 4,may function as decoy receptors for VEGFA. Modulates KDR signalingby forming heterodimers with KDR. Ligand binding leads to theactivation of several signaling cascades. Activation of PLCG leadsto the production of the cellular signaling moleculesdiacylglycerol and inositol 1,4,5-trisphosphate and the activationof protein kinase C. Mediates phosphorylation of PIK3R1, theregulatory subunit of phosphatidylinositol 3-kinase, leading toactivation of phosphatidylinositol kinase and the downstreamsignaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1and the MAP kinase signaling pathway, as well as of the AKT1signaling pathway. Phosphorylates SRC and YES1, and may alsophosphorylate CBL. Isoform 1 phosphorylates PLCG. Promotesphosphorylation of AKT1 at 'Ser-473'. Promotes phosphorylation ofPTK2/FAK1. Isoform 7 has a truncated kinase domain; it increasesphosphorylation of SRC at 'Tyr-418' by unknown means and promotestumor cell invasion..|
|Tissue Specificity||Detected in normal lung, but also in placenta,liver, kidney, heart and brain tissues. Specifically expressed inmost of the vascular endothelial cells, and also expressed inperipheral blood monocytes. Isoform 2 is strongly expressed inplacenta. Isoform 3 is expressed in corneal epithelial cells (atprotein level). Isoform 3 is expressed in vascular smooth musclecells (VSMC)..|
|Sequence Similarities||Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.|
|Subcellular Localization||Isoform 1: Cell membrane; Autophosphorylation promotesubiquitination and endocytosis.|
|Uniprot ID||FLT1: P17948
|Alternative Names||Vascular endothelial growth factor receptor 1; VEGFR-1; 126.96.36.199; Fms-like tyrosine kinase 1; FLT-1; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; FLT; Vascular permeability factor receptor; FLT1; FLT, FRT, VEGFR1;|
|Research Areas||cardiovascular|angiogenesis|growth factors|vegf|vegf receptors|signal transduction|protein phosphorylation|tyrosine kinases|cancer|invasion/microenvironment|angiogenic growth factors|cancer metabolism|response to hypoxia|kits/ lysates/ other|kits|elisa kits|growth factors and hormones elisa kits|cardiovascular elisa kits|pathways and processes|metabolism processes||
Background for Vascular endothelial growth factor receptor 1
Human sVEGFR1/sFLT1 PicoKine™ ELISA Kit Images
Click the images to enlarge.
Intra/Inter Assay Precision
|Intra-Assay Precision||Inter-Assay Precision|
Typical Data Obtained from Human sVEGFR1/sFLT1 PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 15-20min)
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.