|Sample Type:||cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).|
Data & Images
|Product Name||Human TIE2 PicoKine™ ELISA Kit|
|Description||Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TIE2|
|Cite This Product||Human TIE2 PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0519)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Immunogen||Expression system for standard: NSO; Immunogen sequence: A23-K745|
|Cross Reactivity||There is cross-reactivity with human TIE1< 0.1%.|
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
|Sample Type||cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine™ ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
|Capture Antibody||monoclonal antibody from mouse|
|Detection Antibody||polyclonal antibody from goat|
|Natural Levels||Some research articles suggesting the natural levels of TEK are listed below:|
|Storage||Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|-||96-well plate precoated with anti- human TIE2 antibody||1|
|ST0000-10||lyophilized recombinant human TIE2 standard||10ng/tubex2|
|AR1107||biotinylated anti- human TIE2 antibody||130ul(dilution 1:100)|
|AR1103||Avidin-Biotin-Peroxidase Complex(ABC)||130ul(dilution 1:100)|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
*Additional components can be purchased. If you need extra of the above components please order them together to avoid additional shipping charges.See Prices For Extra Components
Material Required But Not Provided
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 4. Clean tubes and Eppendorf tubes.
- 5. Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Angiopoietin-1 receptor|
|Gene Full Name||Angiopoietin-1 receptor|
|Protein Function||Tyrosine-protein kinase that acts as cell-surfacereceptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis,endothelial cell survival, proliferation, migration, adhesion andcell spreading, reorganization of the actin cytoskeleton, but alsomaintenance of vascular quiescence. Has anti-inflammatory effectsby preventing the leakage of proinflammatory plasma proteins andleukocytes from blood vessels. Required for normal angiogenesisand heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibitsangiogenesis, depending on the context. Inhibits angiogenesis andpromotes vascular stability in quiescent vessels, whereendothelial cells have tight contacts. In quiescent vessels,ANGPT1 oligomers recruit TEK to cell-cell contacts, formingcomplexes with TEK molecules from adjoining cells, and this leadsto preferential activation of phosphatidylinositol 3-kinase andthe AKT1 signaling cascades. In migrating endothelial cells thatlack cell-cell adhesions, ANGT1 recruits TEK to contacts with theextracellular matrix, leading to the formation of focal adhesioncomplexes, activation of PTK2/FAK and of the downstream kinasesMAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation ofsprouting angiogenesis. ANGPT1 signaling triggers receptordimerization and autophosphorylation at specific tyrosine residuesthat then serve as binding sites for scaffold proteins andeffectors. Signaling is modulated by ANGPT2 that has loweraffinity for TEK, can promote TEK autophosphorylation in theabsence of ANGPT1, but inhibits ANGPT1-mediated signaling bycompeting for the same binding site. Signaling is also modulatedby formation of heterodimers with TIE1, and by proteolyticprocessing that gives rise to a soluble TEK extracellular domain.The soluble extracellular domain modulates signaling byfunctioning as decoy receptor for angiopoietins. TEKphosphorylates DOK2, GRB7, GRB14, PIK3R1; SHC1 and TIE1..|
|Tissue Specificity||Detected in umbilical vein endothelial cells.Proteolytic processing gives rise to a soluble extracellulardomain that is detected in blood plasma (at protein level).Predominantly expressed in endothelial cells and theirprogenitors, the angioblasts. Has been directly found in placentaand lung, with a lower level in umbilical vein endothelial cells,brain and kidney..|
|Sequence Similarities||Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.|
|Subcellular Localization||Cell membrane; Recruited to cell-cell contacts inquiescent endothelial cells. Colocalizes with the actincytoskeleton and at actin stress fibers during cell spreading.Recruited to the lower surface of migrating cells, especially therear end of the cell. Proteolytic processing gives rise to asoluble extracellular domain that is secreted.|
|Uniprot ID||TEK: Q02763
|Alternative Names||Angiopoietin-1 receptor; 126.96.36.199; Endothelial tyrosine kinase; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; hTIE2; p140 TEK; CD202b; TEK; TIE2, VMCM, VMCM1;|
|Research Areas||Nothing Found||
Background for Angiopoietin-1 receptor
Human TIE2 PicoKine™ ELISA Kit Images
Click the images to enlarge.
Intra/Inter Assay Precision
|Intra-Assay Precision||Inter-Assay Precision|
Typical Data Obtained from Human TIE2 PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 25-30min)
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.