Human TNFRSF14/HVEM PicoKine™ Fast ELISA Kit


SKU FEK1226
Size 96wells/kit, with removable strips.
Reactivity Human
Sample Type cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Sample Volume 100ul per well
Sensitivity <1pg/ml
Assay Range 15.6pg/ml-1000pg/ml

Introduction

Boster's Picokine™ Fast ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Overview

Product Name Human TNFRSF14/HVEM PicoKine™ Fast ELISA Kit
SKU/Catalog Number FEK1226
Storage & Handling Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Size 96wells/kit, with removable strips.
Description The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Human TNFRSF14 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. TNFRSF14 tissue specificity: Widely expressed, with the highest expression in lung, spleen and thymus.
Cite This Product Human TNFRSF14/HVEM PicoKine™ Fast ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1226)
Sample Type cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Anticoagulant(s): heparin or EDTA
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants that are not specified above or contact us to check for feasibility.
Sensitivity <1pg/ml
Assay Range 15.6pg/ml-1000pg/ml
Immunogen Expression system for standard: NSO; Immunogen sequence: L39-V202
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Antibody Clonalities Capture Antibody | Detection Antibody:
monoclonal antibody from mouse|polyclonal antibody from goat

Assay Details

Kit Components

Catalog number Description Quantity
FEK1226-CAP 96-well plate precoated with anti-Human TNFRSF14 antibody 1
FEK1226-ST lyophilized recombinant Human TNFRSF14 standard 10ng/tube
FEK1226-DA biotinylated anti-Human TNFRSF14 antibody 130ul
AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul
AR1106-1 sample diluent buffer 30ml
AR1106-2 antibody diluent buffer 12ml
AR1106-3 ABC diluent buffer 12ml
AR1104 TMB color developing agent 10ml
AR1105 TMB stop solution 10ml
PLA-SEA Adhesive cover 4

*Why there is no wash buffer? Our Avidin-Biotin-Peroxidase Diluent contains the detergent (TWEEN) normally present in other companies' ELISA kits. This saves you the step of having to wash with the special wash buffer and achieve similar or better signal to noise ratio. The wash can use regular wash buffers (PBS, TBS etc.) commonly found in labs.

Materials Required But Not Provided

  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
  • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
  • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Typical Data Obtained from Human TNFRSF14/HVEM PicoKine™ Fast ELISA Kit

(TMB reaction incubate at 37°C for 25-30min)

Concentration(pg/ml)015.631.262.51252505001000
O.D.0.0290.0920.1480.2670.4770.9101.5182.294

Intra/Inter Assay Precision

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3320238932205420
Standard deviation1.585.8%823.341.8511.8927.3
CV(%)4.8%4.1%6%5.8%5.8%6.5%

Reproducibility

Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot1 (pg/ml)Lot2 (pg/ml)Lot3 (pg/ml)Lot4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 133323331320.822.5%
Sample 220220723423221814.376.5%
Sample 338942841338640417.364.2%
*number of samples for each test n=16.

*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q92956
Gene Name TNFRSF14
Protein Name Tumor necrosis factor receptor superfamily member 14
Tissue Specificity Widely expressed, with the highest expression in lung, spleen and thymus.
Alternative Names Tumor necrosis factor receptor superfamily member 14;Herpes virus entry mediator A;Herpesvirus entry mediator A;HveA;Tumor necrosis factor receptor-like 2;TR2;CD270;TNFRSF14;HVEA, HVEM;UNQ329/PRO509;
Subcellular Localization Membrane ; Single-pass type I membrane protein .
Molecular Weight 30392 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells. .
Research Areas Human

*You can search these to find other products in these research areas.
Background Tumor necrosis factor receptor superfamily member 14(TNFRSF14), also known as HVEM, is a protein that in humans is encoded by the TNFRSF14 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. It is mapped to 1p36.32. HVEM plays an important role in HSV pathogenesis because it enhanced the entry of several wildtype HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T cells. HVEM and BTLA which are form a bidirectional signaling pathway can regulate cell survival and inhibitory responses between interacting cells. HVEM as an important orchestrator of mucosal immunity integrates signals from innate lymphocytes to induce optimal epithelial Stat3 activation, which indicated that targeting HVEM with agonists could improve host defense.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Write Your Own Review
Only registered users can write reviews. Please Sign in or create an account
In stock
Order Product
FEK1226
Limited time multi-pack pricing available only to end users in US and Canada.
$419.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.

Write a review for FEK1226