|Sample Type:||cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).|
Data & Images
|Product Name||Human VEGFR2/KDR PicoKine™ ELISA Kit|
|Description||Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human VEGFR2/KDR|
|Cite This Product||Human VEGFR2/KDR PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0544)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Immunogen||Expression system for standard: NSO; Immunogen sequence: A20-E764|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
|Sample Type||cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine™ ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
|Capture Antibody||monoclonal antibody from mouse|
|Detection Antibody||polyclonal antibody from goat|
|Natural Levels||Some research articles suggesting the natural levels of KDR are listed below:|
|Storage||Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|-||96-well plate precoated with anti- human VEGFR2/KDR antibody||1|
|ST0000-10||lyophilized recombinant human VEGFR2/KDR standard||10ng/tubex2|
|AR1107||biotinylated anti- human VEGFR2/KDR antibody||130ul(dilution 1:100)|
|AR1103||Avidin-Biotin-Peroxidase Complex(ABC)||130ul(dilution 1:100)|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
*Additional components can be purchased. If you need extra of the above components please order them together to avoid additional shipping charges.See Prices For Extra Components
Material Required But Not Provided
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 4. Clean tubes and Eppendorf tubes.
- 5. Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Vascular endothelial growth factor receptor 2|
|Gene Full Name||Vascular endothelial growth factor receptor 2|
|Protein Function||Tyrosine-protein kinase that acts as a cell-surfacereceptor for VEGFA, VEGFC and VEGFD. Plays an essential role inthe regulation of angiogenesis, vascular development, vascularpermeability, and embryonic hematopoiesis. Promotes proliferation,survival, migration and differentiation of endothelial cells.Promotes reorganization of the actin cytoskeleton. Isoformslacking a transmembrane domain, such as isoform 2 and isoform 3,may function as decoy receptors for VEGFA, VEGFC and/or VEGFD.Isoform 2 plays an important role as negative regulator of VEGFA-and VEGFC-mediated lymphangiogenesis by limiting the amount offree VEGFA and/or VEGFC and preventing their binding to FLT4.Modulates FLT1 and FLT4 signaling by forming heterodimers. Bindingof vascular growth factors to isoform 1 leads to the activation ofseveral signaling cascades. Activation of PLCG1 leads to theproduction of the cellular signaling molecules diacylglycerol andinositol 1,4,5-trisphosphate and the activation of protein kinaseC. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAPkinase signaling pathway, as well as of the AKT1 signalingpathway. Mediates phosphorylation of PIK3R1, the regulatorysubunit of phosphatidylinositol 3-kinase, reorganization of theactin cytoskeleton and activation of PTK2/FAK1. Required forVEGFA-mediated induction of NOS2 and NOS3, leading to theproduction of the signaling molecule nitric oxide (NO) byendothelial cells. Phosphorylates PLCG1. Promotes phosphorylationof FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC..|
|Tissue Specificity||Detected in cornea (at protein level). Widelyexpressed..|
|Sequence Similarities||Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.|
|Subcellular Localization||Cell junction. Endoplasmicreticulum. Localized with RAP1Aat cell-cell junctions (By similarity). Colocalizes with ERN1 andXBP1 in the endoplasmic reticulum in endothelial cells in avascular endothelial growth factor (VEGF)-dependent manner(PubMed:23529610)..|
|Uniprot ID||KDR: P35968
|Alternative Names||Vascular endothelial growth factor receptor 2; VEGFR-2; 22.214.171.124; Fetal liver kinase 1; FLK-1; Kinase insert domain receptor; KDR; Protein-tyrosine kinase receptor flk-1; CD309; KDR; FLK1, VEGFR2;|
|Research Areas||cardiovascular|angiogenesis|growth factors|vegf|vegf receptors|microbiology|interspecies interaction|host virus interaction|signal transduction|protein phosphorylation|tyrosine kinases|receptor tyrosine kinases|growth factors/hormones|stem cells|hematopoietic progenitors|surface molecules|cancer|invasion/microenvironment|angiogenic growth factors|oncoproteins/suppressors|oncoproteins|growth factor receptors|endothelial progenitors|endothelial markers|cancer metabolism|response to hypoxia|cardiovascular markers|cell markers|endothelial cells|pathways and processes|metabolism processes||
Background for Vascular endothelial growth factor receptor 2
Human VEGFR2/KDR PicoKine™ ELISA Kit Images
Click the images to enlarge.
Intra/Inter Assay Precision
|Intra-Assay Precision||Inter-Assay Precision|
Typical Data Obtained from Human VEGFR2/KDR PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 20-25min)
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.