Hypersensitive ECL Chemiluminescent Substrate

Hypersensitive ECL Chemiluminescent Substrate, Western Blotting Related Reagent Cited in 84 publication(s).

Product Info Summary

SKU AR1170
Pack size 200mL
Applications To detect antigen at low picogram level by reacting with horseradish peroxidase

List of Components

DescriptionQuantityVolumeCatalog Number
Reagent A: Luminol and luminous enhancer1100mL AR1170-A
Reagent B: Peroxide and stabilizer1100mLAR1170-B


Product Name Hypersensitive ECL Chemiluminescent Substrate
SKU/Catalog Number AR1170
Form Supplied Reagent A and B: ready-to-use 1X solutions
Pack Size 200mL ( (sufficient reagents for 2000 cm2 of membrane)
Storage Upon receipt store Hypersensitive ECL Chemiluminescent Substrate at 4°C. Protect from light. It is stable at 4°C for one year.
Detection Method Chemiluminescent
Substrate Type HRP (Horseradish Peroxidase) Substrate
Description Boster’s Hypersensitive ECL Chemiluminescent Substrate is a luminol-based chemiluminescent substrate and it can detect horseradish peroxidase (HRP) at high sensitivity levels (low picogram).
Equivalent Thermofisher (Product No. 32106, 32109, 32209);
Bio-Rad Immu-StarTM HRP Chemiluminescent Kit (Product No. 170-5040, 170-5041, 170-5042, 170-5043, 170-5044, 170-5045, 170-5046, 170-5047)
Cite This Product Hypersensitive ECL Chemiluminescent Substrate (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR1170)
Application To detect antigen at low picogram level by reacting with horseradish peroxidase
*Our Boster Guarantee covers the use of this product in the above tested applications.


Type of ECL Western Blotting SubstrateSensitivity LevelCatalog Number
Hypersensitive ECL Chemiluminescent Substratelow picogramAR1170
ECL Plus Western Blotting Substratelow-picogram to mid-femtogram levelAR1196

Assay Principle

The Boster Hypersensitive ECL Chemiluminescent Substrate is a luminol-based chemiluminescent substrate and it can detect horseradish peroxidase (HRP) at high sensitivity levels (low picogram). Boster Western Blotting Substrate may be used for immunoblots, western blots, dot blots and any blotting application utilizing horseradish peroxidase (HRP)-conjugates. The substrate can be used with various blocking buffers and on nitrocellulose or PVDF membranes. Such blots will exhibit low backgrounds. Produced chemiluminescence can be visualized on CCD imaging systems or x-ray film.

Additional Materials Required

1. Nitrocellulose or PVDF membranes

2. X-ray film or an imaging system

3. Rotary platform shaker for agitation of membrane during incubations

4. Wash Buffer: PBS or TBS containing 0.05-0.1% Tween-20 (PBS: 10mM Na3PO4, 150mM NaCl, pH7.2; TBS: 10mM Tris, 150mM NaCl, pH7.4)

5. Blocking Buffer: Add some blocking medium (e.g. casein, BSA or non-fat milk) into the Wash Buffer for a final blocking medium concentration of 1-5% (w/v)

6. Primary Antibody: Choose an antibody specific to the target proteins. Prepare the antibody stock solution in Wash Buffer or Blocking Buffer

7. HRP-conjugated Secondary Antibody: Choose a HRP-conjugated Secondary Antibody that specifically binds to the primary antibody.

Important Product Information

• For optimal results, use a shaking platform during incubation steps.

• Do not use sodium azide as a preservative for buffers. Sodium azide is an inhibitor of HRP.

• Do not handle the membrane with bare hands. Always wear gloves or use clean forceps.

• All equipment must be clean and free of foreign material. Metallic devices must have no visible signs of rust. Rust may cause speckling and high background.

• Exposure to the sun or any other intense light can harm the substrate. For best results keep the substrate working solution in an amber bottle and avoid prolonged exposure to any intense light. Short-term exposure to typical laboratory lighting will not harm the working solution.

• Empirical testing is essential to determine the appropriate blocking reagent for each Western blot system, as crossreactivity of the blocking reagent with antibodies can cause nonspecific signal and varying system sensitivity.

• When using avidin/biotin systems, avoid using milk as a blocking reagent as milk contains variable amounts of endogenous biotin, which causes high background signal.

• Use sufficient volumes of wash buffer, blocking buffer, antibody solution and substrate working solution to cover the blot and ensure that it never becomes dry. Using large blocking and wash buffer volumes minimizes nonspecific signal.

• Add Tween™ 20 Detergent (final concentration of 0.05-0.1%) to the blocking buffer and all diluted antibody solutions to minimize nonspecific signal.

• Do not use polystyrene vessels to mix and prepare the substrate working solution; this type of plastic causes the solution to become cloudy and produce a precipitate.

• Use new pipette tips separately while pipetting reagent A and B to avoid cross contamination.

Assay Protocol

1. Remove blot from the transfer apparatus and wash membrane with Wash Buffer 3 times for 5 minutes each.

2. Block nonspecific sites with Blocking Buffer for 60 minutes at room temperature with shaking.

3. Remove the blocking buffer and add the primary antibody working solution. Incubate blot for 1 hour at room temperature with shaking or overnight at 2-8°C with shaking.

4. Suspend membrane in Wash buffer and agitate for more than 5 minutes. Replace Wash Buffer at least 4-6 times. Increasing the volume of Wash Buffer, the numbers of washes and wash duration may help minimize background signal.

5. Incubate blot with the HRP conjugated secondary antibody working solution for 1 hour at room temperature with shaking.

6. Repeat Step 3 to remove nonbound HRP conjugate.
Note: The membrane must be thoroughly washed after incubation with the HRP conjugate.

7. Prepare the substrate working solution by mixing Reagent A and Reagent B at 1:1. Use 0.1mL working solution per cm2 of membrane.

8. Incubate blot with working solution for 1-5 minutes at room temperature.

9. Place blot in a clear plastic wrap or transparent plastic sheet protector. Use an absorbent tissue to remove excess liquid and carefully press out any bubbles from between the blot and the membrane protector.

10. Place the protected membrane in a film cassette with the protein side facing up.

11. Place X-ray film on top of the blot membrane. Perform a exposure of 1 minute, Vary the exposure time to achieve optimal results. Light emission is most intense during the first 5-30 minutes after substrate incubation.
Note: CCD detection: Put the blot membrane in the CCD and detect the chemiluminescence image according to the manufacturers’ instructions.

12. Develop and fix the film.


ProblemPossible CauseSolution
Blot glows in the dark room
Membrane has brown or yellow bands
Signal fades quickly
Too much HRP in the sysem Dilute HRP conjugate further
Weak or no signal Used insufficient quantities of antigen or antibodies
Insufficient protein transfer
Low HRP or substrate activity
Increase the amount of antigen or antibodies
Optimize transfer condition
Replace the secondary antibody or substrate
High Background Too much HRP in the sysem
Inadequate blocking or used inappropriate blocking buffer
Insufficient washing
Use too much antigen or antibodies
Use non- specific primary antibody
Overexposed film
Dilute HRP-conjugate further
Optimize blocking conditions
Increase duration, number and volume of washes
Decrease the amount of antigen or antibodies
Replace the antibody
Decrease exposure time
Spots within the protein bands Ineffective protein transfer
Unevenly hydrated membrane
Bubble between X-ray film and membrane
Optimize transfer procedure
Replace the membrane
Remove all bubbles before exposing blot to film
Speckled background Inadequate blocking Optimize blocking conditions
Nonspecific bands Too much HRP in the system
SDS caused nonspecific binding to protein
Use non-specific primary antibody
Inadequate blocking
Dilute HRP conjugate further
Do not use SDS
Replace the antibody
Optimize blocking conditions

Product Images

AR1170 has been cited in 84 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Li HN,Yang QQ,Wang WT,Tian X,Feng F,Zhang ST,Xia YT,Wang JX,Zou YW,Wang JY,Zeng XY.Red nucleus IL-33 facilitates the early development of mononeuropathic pain in male rats by inducing TNF-α through activating ERK, p38 MAPK, and JAK2/STAT3.J Neuroinflammation.2021 Jul 5;18(1):150.doi:10.1186/s12974-021-02198-9.PMID:34225736;PMCID:PMC8258957.
Species: Rat
AR1170 usage in article: APP:WB, SAMPLE:RN TISSUE, DILUTION:NA

Liu,B.,Dong,J.,Wang,S.,Yu,H.,Li,Z.,Sun,P.,& Zhao,L.(2021).Helicobacter pylori causes delayed gastric emptying by decreasing interstitial cells of Cajal.Experimental and Therapeutic Medicine,22,663.https://doi.org/10.3892/etm.2021.10095
Species: Mouse

Chen T,Lei S,Zeng Z,Zhang J,Xue Y,Sun Y,Lan J,Xu S,Mao D,Guo B.Linc00261 inhibits metastasis and the WNT signaling pathway of pancreatic cancer by regulating a miR‑552‑5p/FOXO3 axis.Oncol Rep.2020 Mar;43(3):930-942.doi:10.3892/or.2020.7480.Epub 2020 Jan 27.PMID:32020223;PMCID:PMC7041108.
Species: Human,Mouse
AR1170 usage in article: APP: WB, SAMPLE:PC CELLS, DILUTION:NA

Zhang T,Liu C,Yu Y,Geng J,Meng Q,Xu S,Zhou F,Chen Y,Jin S,Shen J,Pan B,Meng F,Liu F. TBL1XR1 is involved in c-Met-mediated tumorigenesis of human nonsmall cell lung cancer.Cancer Gene Ther.2020 Apr;27(3-4):136-146.doi:10.1038/s41417-019-0111-0.Epub 2019 Jun 27.PMID:31243347.
Species: Human

Li Y, Guo F, Chen T, Zhang L, Qin Y. Anthraquinone derivative C10 inhibits proliferation and cell cycle progression in colon cancer cells via the Jak2/Stat3 signaling pathway. Toxicol Appl Pharmacol. 2021 Mar 12:115481. doi: 10.1016/j.taap.2021.115481. Epub ahead of print. PMID: 33722666.
Species: Human
AR1170 usage in article: APP:WB, SAMPLE:HCT116 CELL AND HT29 CELL, DILUTION:NA

Xu X,Zong K,Wang X,Dou D,Lv P,Zhang Z,Li H.miR-30d suppresses proliferation and invasiveness of pancreatic cancer by targeting the SOX4/PI3K-AKT axis and predicts poor outcome.Cell Death Dis.2021 Apr 6;12(4):350.doi:10.1038/s41419-021-03576-0.PMID:33824274.
Species: Human,Mouse

Gan J,Li S,Meng Y,Liao Y,Jiang M,Qi L,Li Y,Bai Y.The influence of photodynamic therapy on the Warburg effect in esophageal cancer cells.Lasers Med Sci.2020 Oct;35(8):1741-1750.doi:10.1007/s10103-020-02966-8.Epub 2020 Feb 8.PMID:32034563.
Species: Human
AR1170 usage in article: APP:WB, SAMPLE:ECA109 CELL AND EC9706 CELL, DILUTION:NA

Chu X,Yu T,Huang X,Xi Y,Ni B,Zhang R,You H.Tomatidine suppresses inflammation in primary articular chondrocytes and attenuates cartilage degradation in osteoarthritic rats.Aging (Albany NY).2020 Jul 5;12(13):12799-12811.doi:10.18632/aging.103222.Epub 2020
Species: Rat

Guochun Ou,Qin Liu,Chengxiu Yu,Xiaoju Chen,Wenbo Zhang,Yong Chen,Tao Wang,Yongqiang Luo,Guolu Jiang,Mingmei Zhu,Hongmei Li,Mei Zeng,"The Protective Effects of Maresin 1 in the OVA-Induced Asthma Mouse Model",Mediators of Inflammation,vol.2021,Article ID 4
Species: Mouse

Chen B,Chai Q,Xu S,Li Q,Wu T,Chen S,Wu L.Silver nanoparticle-activated COX2/PGE2 axis involves alteration of lung cellular senescence in vitro and in vivo.Ecotoxicol Environ Saf.2020 Nov;204:111070.doi:10.1016/j.ecoenv.2020.111070.Epub 2020 Aug 4.PMID:327
Species: Human,Mouse
AR1170 usage in article: APP:WB, SAMPLE:MRC5 CELL, DILUTION:NA

Bi,F.,Chen,C.,Fu,J.,Yu,L.,& Geng,J.(2021).Inhibiting proliferation and metastasis of osteosarcoma cells by downregulation of long non‑coding RNA colon cancer‑associated transcript 2 targeting microRNA‑143.Oncology Letters,21,265.https://doi.org/10.3892/ol
Species: Human

Bo Bai,Dongfang Li,Guofang Xue,Peng Feng,Meiqin Wang,Yudi Han,Yanan Wang,Christian Hölscher,The novel GLP-1/GIP dual agonist DA3-CH is more effective than liraglutide in reducing endoplasmic reticulum stress in diabetic rats with cerebral ischemia-reperfu
Species: Rat

Heshen Tian,Lei Liu,Ying Wu et al.Resistin-like Molecule β Acts as a Mitogenic Factor in Hypoxic Pulmonary Hypertension via the Ca2+-dependent PI3K/Akt/mTOR and PKC/MAPK Signaling Pathways, 04 December 2020, PREPRINT (Version 3) available at Research Squa
Species: Rat

Juan Wang,Lei Wang,Wenting Zhao et al. Aged Zinc Oxide Nanoparticles Did Not Induce Cytotoxicity Through Apoptosis Signaling Pathway as Fresh NPs, 23 December 2020, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-132318
Species: Human,Hamster
AR1170 usage in article: APP:WB, SAMPLE:AL CELL, DILUTION:NA

Guofang Xing, Pingting Huo, Xinyi Zhang et al. Proteomics Analysis of the Protective Effects of Hesperetin on Coronary Artery injury in Streptozotocin-Induced Type 1 Diabetic Rats, 10 December 2020, PREPRINT (Version 1) available at Research Square [+http
Species: Rat

Wang Q,Suo Y,Wang X,Wang Y,Tian X,Gao Y,Liu N,Liu R.Study on the mechanism of photodynamic therapy mediated by 5-aminoketovalerate in human ovarian cancer cell line.Lasers Med Sci.2021 Jan 3.doi:10.1007/s10103-020-03226-5.Epub ahead of print.PMID:33392781
Species: Human
AR1170 usage in article: APP:WB, SAMPLE:OVCAR3 CELL, DILUTION:NA

Wei W,Hu Z,Jia Y,Gu T,Zhao W,Ji S. Characterization of lamin B receptor of Sf9 cells and its fate during Autographa californica nucleopolyhedrovirus infection. Cytotechnology.2020 Apr;72(2):315-325.doi:10.1007/s106 16-020-00380-0.Epub 2020 Apr 3.PMID:3224
Species: Spodoptera frugiperda
AR1170 usage in article: APP:WB, SAMPLE:SF9 CELL, DILUTION:NA

Ni B,Huang X,Xi Y,Mao Z,Chu X,Zhang R,Ma X,You H.Neferine Inhibits Expression of Inflammatory Mediators and Matrix Degrading Enzymes in IL-1β-Treated Rat Chondrocytes via Suppressing MAPK and NF-κB Signaling Pathways.Inflammation.2020 Aug;43(4):1209-1221.
Species: Rat

Sun K,Luo J,Jing X,Xiang W,Guo J,Yao X,Liang S,Guo F,Xu T.Hyperoside ameliorates the progression of osteoarthritis: An in vitro and in vivo study.Phytomedicine.2020 Oct 14;80:153387.doi:10.1016/j.phymed.2020. 153387.Epub ahead of print.PMID:33130473.
Species: Mouse

Zhang J,Fu ZL,Chu ZX,Song BW.Gastroprotective Activity of the Total Flavones from Abelmoschus manihot (L.) Medic Flowers. Evid Based Complement Alternat Med. 2020 Feb 24;2020:6584945.doi: 10.1155/2020/6584945.PMID:32184895;PMCID:PMC7060849.
Species: Abelmoschus manihot (L.) Medic, Mouse

Sequencing and Expression Characterization of Antifreeze Protein Maxi-Like in Apis cerana cerana

The Optimization of Soluble PTEN Expression in Escherichia coli

Expressional and functional interactions of two Apis cerana cerana olfactory receptors

MicroRNA-27a protects retinal pigment epithelial cells under high glucose conditions by targeting TLR4

Characterization of an Atypical Metalloproteinase Inhibitors Like Protein (Sbp8-1) From Scallop Byssus

MicroRNA-206 regulates the epithelial-mesenchymal transition and inhibits the invasion and metastasis of prostate cancer cells by targeting Annexin A2

5-Hydroxy-4?-Nitro-7-Propionyloxy-Genistein Inhibited Invasion and Metastasis via Inactivating Wnt/?-Catenin Signal Pathway in Human Endometrial Carcinoma Ji Endometrial Cells

Effects of celecoxib on cell apoptosis and Fas, FasL and Bcl-2 expression in a BGC-823 human gastric cancer cell line

The protective effects of Resveratrol against radiation-induced intestinal injury

Adiponectin attenuates high glucose-induced apoptosis through the AMPK/p38 MAPK signaling pathway in NRK-52E cells

AhR-E2F1-KGFR signaling is involved in KGF-induced intestinal epithelial cell proliferation

Fermented Chinese Formula Shuan-Tong-Ling Protects Brain Microvascular Endothelial Cells against Oxidative Stress Injury

Epoxyeicosanoids prevent intervertebral disc degeneration?in vitro?and?in vivo

miR-138-5p suppresses autophagy in pancreatic cancer by targeting SIRT1

Smad3-related miRNAs regulated oncogenic TRIB2 promoter activity to effectively suppress lung adenocarcinoma growth

Disrupted in schizophrenia 1 (DISC1) inhibits glioblastoma development by regulating mitochondria dynamics

miR-20b Inhibits T Cell Proliferation and Activation via NFAT Signaling Pathway in Thymoma-Associated Myasthenia Gravis

Monoacylglycerol Lipase: A Novel Potential Therapeutic Target and Prognostic Indicator for Hepatocellular Carcinoma

Downregulation of ZEB2-AS1 decreased tumor growth and metastasis in hepatocellular carcinoma

CXCL12/CXCR4 Axis Regulates Aggrecanase Activation and Cartilage Degradation in a Post-Traumatic Osteoarthritis Rat Model

Ocular Albinism Type 1 Regulates Melanogenesis in Mouse Melanocytes

Hepcidin in non-alcoholic fatty liver disease regulated by the TLR4/NF-?B signaling pathway

siRNA targeting RBP2 inhibits expression, proliferation, tumorigenicity and invasion in thyroid carcinoma cells

Amlexanox Suppresses Osteoclastogenesis and Prevents Ovariectomy-Induced Bone Loss

Electromagnetic radiation at 900?MHz induces sperm apoptosis through bcl-2, bax and caspase-3 signaling pathways in rats

RUNX2 RNA interference inhibits the invasion of osteosarcoma

Adenovirus-mediated transfection with glucose transporter 3 suppresses PC12 cell apoptosis following ischemic injury

Down-regulation of Homer1b/c expression protects cultured neurons after traumatic injury

Expression profiles of microRNAs from lactating and non-lactating bovine mammary glands and identification of miRNA related to lactation

PLK1 gene suppresses cell invasion of undifferentiated thyroid carcinoma through the inhibition of CD44v6, MMP-2 and MMP-9

A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus).

Effect of Buddleia flavonoids drug-containing plasma on the expression of STAT1 phosphoprotein in lacrimal gland epithelial cells in vitro

RNAi-mediated silencing of CD147 inhibits tumor cell proliferation, invasion and increases chemosensitivity to cisplatin in SGC7901 cells in vitro

Xue D, Xue Y, Niu Z, Guo X, Xu C. Genet Mol Biol. 2017 Oct-Dec;40(4):855-859. doi: 10.1590/1678-4685-GMB-2017-0029. Epub 2017 Nov 6. Expression analysis on 14-3-3 proteins in regenerative liver following partial hepatectomy

Guo X, Fu Y, Wang Z, Wang T, Li C, Huang T, Gao F, Li C. Oxid Med Cell Longev. 2018 Mar 25;2018:4950705. doi: 10.1155/2018/4950705. eCollection 2018. Di-2-pyridylhydrazone Dithiocarbamate Butyric Acid Ester Exerted Its Proliferative Inhibition aga...

Li M, Zhou J, Jin W, Li X, Zhang Y. Front Pharmacol. 2018 Apr 6;9:308. doi: 10.3389/fphar.2018.00308. eCollection 2018. Danhong Injection Combined With t-PA Improves Thrombolytic Therapy in Focal Embolic Stroke

Xu XD, Zhao Y, Zhang M, He RZ, Shi XH, Guo XJ, Shi CJ7, Peng F8, Wang M9, Shen M0, Wang X, Li X, Qin RY. Int J Mol Sci. 2017 Feb 10;18(2). pii: E370. doi: 10.3390/ijms18020370. Inhibition of Autophagy by Deguelin Sensitizes Pancreatic Cancer Cells...

Wang J, Guo XJ, Ding YM, Jiang JX. World J Gastroenterol. 2017 Mar 7;23(9):1594-1601. doi: 10.3748/wjg.v23.i9.1594. miR-1181 inhibits invasion and proliferation via STAT3 in pancreatic cancer

Mei ZG, Tan LJ, Wang JF, Li XL, Huang WF, Zhou HJ. Neural Regen Res. 2017 Mar;12(3):425-432. doi: 10.4103/1673-5374.202946. Fermented Chinese formula Shuan-Tong-Ling attenuates ischemic stroke by inhibiting inflammation and apoptosis

Wu M1, Huang C2, Huang X1, Liang R3, Feng Y1, Luo X1. Oncol Rep. 2017 Oct;38(4):2173-2181. doi: 10.3892/or.2017.5900. Epub 2017 Aug 11. MicroRNA-144-3p suppresses tumor growth and angiogenesis by targeting SGK3 in hepatocellular carcinoma

Yan J, Wang J, Huang H, Huang Y, Mi T, Zhang C, Zhang L. Am J Transl Res. 2017 Oct 15;9(10):4492-4501. eCollection 2017. Fibroblast growth factor 21 delayed endothelial replicative senescence and protected cells from H2O2-induced premature senesce...

Liu B, Liu C, Cong W, Li N, Zhou N, Tang Y, Wei C, Bai H, Zhang Y, Xiao J. Skelet Muscle. 2017 May 11;7(1):8. doi: 10.1186/s13395-017-0126-x. Retinoid acid-induced microRNA-31-5p suppresses myogenic proliferation and differentiation by targeting C...

Wang R, Shen Z, Yang L, Yin M, Zheng W, Wu B, Liu T, Song H. Int J Mol Med. 2017 Nov;40(5):1537-1548. doi: 10.3892/ijmm.2017.3121. Epub 2017 Sep 6. Protective effects of heme oxygenase-1-transduced bone marrow-derived mesenchymal stem cells on red...

Feng XE, Liang TG, Gao J, Kong P, Ge R, Li QS,. Oxid Med Cell Longev. 2017;2017:7028478. doi: 10.1155/2017/7028478. Epub 2017 Jun 14. Heme Oxygenase-1, a Key Enzyme for the Cytoprotective Actions of Halophenols by Upregulating Nrf2 Expression via ...

Tang S, Pan H, Wei W, Yang H, Liu J, Yang R. Oncotarget. 2017 Oct 19;8(62):105155-105169. doi: 10.18632/oncotarget.21927. eCollection 2017 Dec 1. GOLPH3: a novel biomarker that correlates with poor survival and resistance to chemotherapy in breast...

Wu YX, Sun Y, Ye YP, Zhang P, Guo JC, Huang JM, Jing XZ, Xiang W, Yu SY, Guo FJ. Mol Med Rep. 2017 Dec;16(6):8200-8208. doi: 10.3892/mmr.2017.7648. Epub 2017 Sep 28. Iguratimod prevents ovariectomy-induced bone loss and suppresses osteoclastogenes...

Huang JM, Bao Y, Xiang W, Jing XZ, Guo JC, Yao XD, Wang R, Guo FJ. Evid Based Complement Alternat Med. 2017;2017:8085325. doi: 10.1155/2017/8085325. Epub 2017 Dec 27. Icariin Regulates the Bidirectional Differentiation of Bone Marrow Mesenchymal S...

Guo J, Li W, Wu Y, Jing X, Huang J, Zhang J, Xiang W, Ren R, Lv Z, Xiao J, Guo F. Front Pharmacol. 2017 Oct 4;8:693. doi: 10.3389/fphar.2017.00693. eCollection 2017. Meclizine Prevents Ovariectomy-Induced Bone Loss and Inhibits Osteoclastogenesis ...

Li YF, Yuan YY, Zhang YM, Zhao N, Zhang Q, Meng FX, Gao RP, Yu BF, Zhang YH, Guo R, Wang HL, Xie J, Xu J, Qin Q, Dong XS. Mol Med Rep. 2017 Jul;16(1):764-772. doi: 10.3892/mmr.2017.6657. Epub 2017 May 31. HSVtk/GCV system on hepatoma carcinoma cel...

Liang B, Xiao T, Long J, Liu M, Li Z, Liu S, Yang J. Int J Mol Med. 2017 Dec;40(6):1781-1791. doi: 10.3892/ijmm.2017.3191. Epub 2017 Oct 16. Hydrogen sulfide alleviates myocardial fibrosis in mice with alcoholic cardiomyopathy by downregulating au...

Chen W, Liu X, Yuan S, Qiao T. Oncol Lett. 2018 Mar;15(3):3883-3889. doi: 10.3892/ol.2018.7800. Epub 2018 Jan 16. HSPA12B overexpression induces cisplatin resistance in non-small-cell lung cancer by regulating the PI3K/Akt/NF-κB signaling pathway

Bai J, Yang BJ, Luo X. Oncol Lett. 2018 Apr;15(4):5227-5235. doi: 10.3892/ol.2018.7938. Epub 2018 Feb 2. Effects of 5-hydroxy-4

Yuan X, Zhang Z, Jiang K, Wang X, Li Y. Med Sci Monit. 2018 Feb 21;24:1080-1088. Preliminary Study of the Role F-Box Protein 32 (FBXO32) in Colorectal Neoplasms Through the Transforming Growth Factor beta (TGF-β)/Smad4 Signalling Pathway

Wang P, Mao Z, Pan Q, Lu R, Huang X, Shang X, Zhang R, You H. Int J Mol Med. 2018 Apr;41(4):2117-2127. doi: 10.3892/ijmm.2018.3410. Epub 2018 Jan 22. Histone deacetylase-4 and histone deacetylase-8 regulate interleukin-1β-induced cartilage catabol...

Liang, Y., Song, D.Z., Liang, S., Zhang, Z.F., Gao, L.X., & Fan, X.H. (2017). The hemagglutinin-neuramidinase protein of Newcastle disease virus upregulates expression of the TRAIL gene in murine natural killer cells through the activation of Syk ...

Ma, Z., Cai, H., Zhang, Y., Chang, L., & Cui, Y. (2017). MiR-129-5p inhibits non-small cell lung cancer cell stemness and chemoresistance through targeting DLK1. Biochemical and Biophysical Research Communications. Advance online publication. doi:...

Zhang Yx, Yue Z, Wang Py, Li Yj, Xin Jx, Pang M, Zheng Qy, Xie Sy. Biomed Pharmacother. 2013 Mar;67(2):97-102. Doi: 10.1016/J.Biopha.2012.11.008. Epub 2012 Dec 28. Cisplatin Upregulates Msh2 Expression By Reducing Mir-21 To Inhibit A549 Cell Growth.

Zhao Y, Wang P, Meng J, Ji Y, Xu D, Chen T, Fan R, Yu X, Yao J,, Dong C. Int J Mol Sci. 2015 May 14;16(5):10921-33. Doi: 10.3390/Ijms160510921. Microrna-27A-3P Inhibits Melanogenesis In Mouse Skin Melanocytes By Targeting Wnt3A.

Liu Zh, Fan W, Chen Rc. Int J Clin Exp Pathol. 2015 Apr 1;8(4):3441-50. Ecollection 2015. 3,4-Dihydroxyphenylethanol Suppresses Irradiation-Induced Pulmonary Fibrosis In Adult Rats.

Zhou X, Li Yj, Gao Sy, Wang Xz, Wang Py, Yan Yf, Xie Sy, Lv Cj. J Cell Mol Med. 2015 May;19(5):1103-13. Doi: 10.1111/Jcmm.12506. Epub 2015 Feb 20. Sulindac Has Strong Antifibrotic Effects By Suppressing Stat3-Related Mir-21.

Chen N, Wu L, Yuan H, Wang J. Int J Biol Sci. 2015 May 30;11(7):833-44. Doi: 10.7150/Ijbs.10564. Ecollection 2015. Ros/Autophagy/Nrf2 Pathway Mediated Low-Dose Radiation Induced Radio-Resistance In Human Lung Adenocarcinoma A549 Cell.

Wang J, Xie Y, Feng Y, Zhang L, Huang X, Shen X, Luo X. Int J Oncol. 2015 Apr;46(4):1507-15. Doi: 10.3892/Ijo.2015.2869. Epub 2015 Feb 3. (-)-Epigallocatechingallate Induces Apoptosis In B Lymphoma Cells Via Caspase-Dependent Pathway And Bcl-2 Fam...

Shen J, Shen S, Das Un, Xu G. Lipids Health Dis. 2012 Jul 10;11:90. Doi: 10.1186/1476-511X-11-90. Effect Of Essential Fatty Acids On Glucose-Induced Cytotoxicity To Retinal Vascular Endothelial Cells.

Xu Xt, Tao Zz, Song Qb, Yao Y, Ruan P. Exp Ther Med. 2012 Sep;4(3):465-468. Epub 2012 Jun 8. Sirna Targeting Decoy Receptor 3 Enhances The Sensitivity Of Gastric Carcinoma Cells To 5-Fluorouracil.

Have you used Hypersensitive ECL Chemiluminescent Substrate?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Hypersensitive ECL Chemiluminescent Substrate

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for Hypersensitive ECL Chemiluminescent Substrate


Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product



Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.