IL-27 Interleukin-27 Human Recombinant Protein

IL-27 Human Recombinant produced in HEK cells is a glycosylated non-covalently heterodimer that consists of 2 chains, IL27A (IL27p28) having a Mw of 25kDa and IL27B (EBI3) having a Mw of 30kDa. Each chain migrates separately on SDS-PAGE.

Product Info Summary

SKU: PROTQ8NEV9
Size: 1ug, 5ug, 100ug
Origin Species: Mouse
Source: HEK.
Application: Cell Culture

Product Name

IL-27 Interleukin-27 Human Recombinant Protein

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SKU/Catalog Number

PROTQ8NEV9

Size

1ug, 5ug, 100ug

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Description

IL-27 Human Recombinant produced in HEK cells is a glycosylated non-covalently heterodimer that consists of 2 chains, IL27A (IL27p28) having a Mw of 25kDa and IL27B (EBI3) having a Mw of 30kDa. Each chain migrates separately on SDS-PAGE. The IL-27 protein is purified by proprietary chromatographic techniques

Storage & Handling

Lyophilized IL-27 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL27 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Cite This Product

IL-27 Interleukin-27 Human Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ8NEV9)

Formulation

The IL-27 was lyophilized from 1mg/ml in 1xPBS.

Predicted MW

27.493kDa

Amino Acid Sequence

IL-27A (215aa):FPRPPGRPQL SLQELRREFT VSLHLARKLL AEVRGQAHRF AESHLPGVNL YLLPLGEQLP DVSLTFQAWR RLSDPERLCF ISTTLQPFHA LLGGLGTQGR WTNMERMQLW AMRLDLRDLQ RHLRFQVLAA GFNLPEEEEE EEEEEEEERK GLLPGALGSA LQGPAQVSWP QLLSTYRLLH SLELVLSRAV RELLLLSKAG HSVWPLGFPT LSPQP.IL-27B (209aa):RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV GPIEATSFIL RAVRPRARYY VQVAAQDLTD YGELSDWSLP ATATMSLGK.

Biological Activity

The specific activity was determined by the dose dependent inhibition of HIV replication in human CD4+ T cells and in human monocyte-derived macrophages and is typically 4-12ng/ml.

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Validation Images & Assay Conditions

Gene/Protein Information For IL27 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q8NEV9

Gene ID

246778

Gene Name

IL27

Full Name

Interleukin-27 subunit alpha

Weight

27.493kDa

Superfamily

IL-6 superfamily

Alternative Names

IL-27 p28 subunit; IL-27 subunit alpha; IL27; IL-27; IL27A; IL-27-A; IL27-A; IL27p28; IL30; interleukin 27; interleukin 30; interleukin-27 subunit alpha; MGC71873; p28IL-27A IL27 IL-27, IL-27AA, IL27p28, IL30, p28, IL27 interleukin 27 interleukin-27 subunit alpha|IL-27 p28 subunit|IL-27 subunit alpha|IL-27-A|IL27-A|interleukin-30

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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