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|Description:||Transient overexpression lysate of intersectin 1 (SH3 domain protein) (ITSN1)|
Intersectin 1 (ITSN1) Human Over-expression Lysates
Transient overexpression lysate of intersectin 1 (SH3 domain protein) (ITSN1)
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Intersectin 1 (ITSN1) Human Over-expression Lysates (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006453)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
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