Product Info Summary
SKU: | PROTQ6SJ61 |
---|---|
Size: | 1mg, 10mg, 50mg |
Source: | Escherichia coli |
Product info
Product Name
LACTB Beta Lactamase E.coli Recombinant Protein
SKU/Catalog Number
PROTQ6SJ61
Size
1mg, 10mg, 50mg
Description
Recombinant E.coli Beta-Lactamase produced in E. coli is a single, non-glycosylated polypeptide chain containing 264 amino acids and having a molecular mass of approximately 28.9 kDa. Beta Lactamase is purified by proprietary chromatographic techniques.
Storage & Handling
Lyophilized Beta Lactamase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Beta Lactamase Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Cite This Product
LACTB Beta Lactamase E.coli Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ6SJ61)
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a concentrated solution in 100mM Tris, pH7.0.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Reconstitution
Reconstitute the lyophilized Beta Lactamase in sterile 18MΩ-cm H2O at 100 µg/ml concentration, which can be further diluted to other aqueous solutions. The Beta Lactamase should be used in pH 7.0-8.0 with temperature not higher than 45°C.
Amino Acid Sequence
MHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW
Biological Activity
One unit will hydrolyze 1.0 μmole of benzyl penicillin at pH 7.0 at 25°C, in presence of EDTA.
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Assay dilution & Images
Reconstitution
Reconstitute the lyophilized Beta Lactamase in sterile 18MΩ-cm H2O at 100 µg/ml concentration, which can be further diluted to other aqueous solutions. The Beta Lactamase should be used in pH 7.0-8.0 with temperature not higher than 45°C.
Validation Images & Assay Conditions
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Recombinant protein fun image
Specific Publications For LACTB Beta Lactamase E.coli Recombinant Protein (PROTQ6SJ61)
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