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SKU AR0101
Pack size 100ml
Form Solution (liquid)
Applications Immunoassays: WB, ELISA, RIA, immunoprecipitation; reporter assays; protein kinase assays; protein purifications; folding studies; chromatographic studies; DNA-protein interaction assays
Price: $100.00
Quantity:

Features/Benefits

Boster's Mammalian Tissue Protein Extraction Reagent features:

  • 1. Small scale, simple, reagent based no need for physical, heating procedures. No need for special equipment.
  • 2. Compatible with cultured cells and tissues.
  • 3. Compatible with WB, SDS-page, IP and BCA/protein assays.
  • 4. One hour, simple and fast.
  • 5. Low cross contamination <10%

Overview

Product Name Mammal Tissue Protein Extraction Reagent
Synonyms Mammal tissue protein solubilizing reagent; Mammal tissue cell detergent lysis reagent; M-PER Mammalian Protein Extraction Reagent
Description Mammal Tissue Protein Extraction Reagent is a ready-to-use Western blot related reagent solution used for efficient extraction of total soluble protein in nondenatured state from animal tissues by homogenization and cell lysis utilizing a dialyzable mild detergent at low concentration, for maximal protein solubilization and minimal interference with protein biological activity enabling direct use in downstream analysis.
Application Immunoassays: WB, ELISA, RIA, immunoprecipitation; reporter assays; protein kinase assays; protein purifications; folding studies; chromatographic studies; DNA-protein interaction assays
Pack Size 100ml, enough for 10g of samples
Reagent Type Universal tissue extraction buffer, detergent solution, cell lysis buffer
Content 100 ml Mammal Tissue Protein Extraction Reagent
Usage Obtaining homogenous tissue cell lysates with soluble protein extracts

Properties

Product Type Ready to use
Form Supplied Solution
Physical State Liquid
Pack Size 100 ml
Recommended working concentration 1ml of extraction reagent per 0.1g of tissuе for tissue samples homogenization
pH 7.6, physiological
Reactivity/severeness Bland, minimal amount of detergent, detergent is dialyzable
Enzymatic Activity None; use additives to add function
Additional components compatibility Compatible with additives, e.g. protease inhibitors (PMSF), reducing agents, chelating agents, salts
Downstream methods compatibility Compatible with standard protein detection methods; immunoassays; enzyme assays; common reporter assays
Storage Store at 4˚C for frequent use, at -20˚C for at least one year.
Precautions FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE

Usage and Handling

1. Place the fresh tissue into pre-cooling normal saline and wash several times to clean the blood on the surface.
2. Weigh the tissue samples, cut into pieces before adding to a glass homogenizer.
3. Prepare tissue samples for homogenization in extraction reagent, using 1ml reagent per 0.1g of tissue. Reduce the amount of extraction reagent if need protein samples with higher concentration.
4. Add 100mmol/ml PMSF before using and dilute to 1m mol/ml.
5. Homogenize using a glass homogenizer until the tissue samples were lysed completely.
6. Increase the amount of extraction reagent if the samples were not lysed completely;
7. Centrifuge for 3- 5 minutes at 10000- 14000 g.
8. Get the supernatant solution, and move to the next steps.

Background

Extraction of tissue proteins being an essential step in the process of protein sample preparation is critical to successful protein analysis. Successful tissue protein extraction requires efficient cell lysis and protein solubilization, while preserving protein integrity and immunoreactivity. The varying tissue composition in different tissue samples implies different approaches for efficient and homogenous tissue protein extraction. The Mammal Tissue Protein Extraction Reagent was designed for use with all tissues, independent of mechanical extraction methods. A mild nondenaturing and easily dialyzable detergent is added to the solution that aids in dissolving cell membranes and enhances solubilization of total protein without compromising function, and the neutral pH and salt concentration of the reagent enable more efficient extraction from cellular compartments yielding homogeneous lysates. The produced cell lysates are directly compatible with reporter gene expression assays (luciferase, beta-galactosidase, chloramphenicol acetyl transferase, CAT, alkaline phosphatase), protein kinase assays (PKA, PKC, tyrosine kinase), phosphatase assays (general phosphatases, tyrosine phosphatases), immunoassays ( Western blots, ELISAs, RIAs, immunoprecipitation), Coomassie-Blue and silver staining, protein purification procedures, electrophoresis, folding studies, chromatographic studies, DNA-protein interaction assays (gel-shift assays), and many other downstream applications.

Mammalian Tissue Protein Extraction Reagent Images

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Mammalian Tissue Protein Extraction Reagent
Mammal Tissue Protein Extraction Reagent (AR0101)
100 mL per pack, Solution (liquid)
Mammalian Tissue Protein Extraction Reagent
Usage and Handling
1. Place the fresh tissue into pre-cooling normal saline and wash several times to clean the blood on the surface.
2. Weigh the tissue samples, cut into pieces before adding to a glass homogenizer.
3. Prepare tissue samples for homogenization in extraction reagent, using 1ml reagent per 0.1g of tissue. Reduce the amount of extraction reagent if need protein samples with higher concentration.
4. Add 100mmol/ml PMSF before using and dilute to 1m mol/ml.
5. Homogenize using a glass homogenizer until the tissue samples were lysed completely.
6. Increase the amount of extraction reagent if the samples were not lysed completely;
7. Centrifuge for 3- 5 minutes at 10000- 14000 g.
8. Get the supernatant solution, and move to the next steps.
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Publications

Jin, G., Guo, L., Zhang, Y., Xue, Y., Zhang, X., Wang, X.,…, & Cheng, J. (2017). Expression and localization of lipocalin-type-prostaglandin D synthase in the goat testis, epididymis and sperm. Small Ruminant Research, 154, 1-4. Advance online publication. doi: 10.1016/j.smallrumres.2017.06.020
Zhu ZX, Sun CC, Ting Zhu Y, Wang Y, Wang T, Chi LS, Cai WH, Zheng JY, Zhou X, Cong WT, Li XK, Jin LT. Exp Cell Res. 2017 Mar 28. pii: S0014-4827(17)30181-7. doi: 10.1016/j.yexcr.2017.03.054. Hedgehog signaling contributes to basic fibroblast growth factor-regulated fibroblast migration.
Zhu Z, Huang Y, Lv L, Tao Y, Shao M, Zhao C, Xue M, Sun J, Niu C, Wang Y, Kim S, Cong W, Mao W, Jin L. J Cell Physiol. 2017 Mar 28. doi: 10.1002/jcp.25934. Acute Ethanol Exposure-induced Autophagy-mediated Cardiac Injury via Activation of the ROS-JNK-Bcl-2 Pathway.
Gui J, Xiong F, Yang W, Li J, Huang G. Am J Reprod Immunol. 2012 May;67(5):383-90. Doi: 10.1111/J.1600-0897.2011.01097.X. Epub 2012 Jan 9. Effects Of Acupuncture On Lif And Il-12 In Rats Of Implantation Failure.
Gui J, Xiong F, Li J, Huang G. Evid Based Complement Alternat Med. 2012;2012:893023. Doi: 10.1155/2012/893023. Epub 2012 Jan 23. Effects Of Acupuncture On Th1, Th2 Cytokines In Rats Of Implantation Failure.
Chen Zy, Li J, Huang Gy. J Huazhong Univ Sci Technolog Med Sci. 2013 Apr;33(2):293-302. Doi: 10.1007/S11596-013-1114-Y. Epub 2013 Apr 17. Effect Of Bushen Yiqi Huoxue Recipe On Placental Vasculature In Pregnant Rats With Fetal Growth Restriction I...
Zhou J, Qu C, Sun Q, Wu L, Liu Y, Yang Z, Zhang J. Chem Biol Interact. 2014 Aug 5;219:57-63. Doi: 10.1016/J.Cbi.2014.05.009. Epub 2014 May 28. Sophoricoside Fails The Embryo Implantation By Compromising The Uterine Endometrial Receptivity At Impla...
Gao D, Ding F, Lei G, Luan G, Zhang S, Li K, Wang D, Zhang L, Dai D. Mol Med Rep. 2015 Apr;11(4):3009-14. Doi: 10.3892/Mmr.2014.3111. Epub 2014 Dec 18. Effects Of Focal Mild Hypothermia On Thrombin-Induced Brain Edema Formation And The Expression ...
Cai Hy, H??lscher C, Yue Xh, Zhang Sx, Wang Xh, Qiao F, Yang W, Qi Js. Neuroscience. 2014 Sep 26;277:6-13. Doi: 10.1016/J.Neuroscience.2014.02.022. Epub 2014 Feb 27. Lixisenatide Rescues Spatial Memory And Synaptic Plasticity From Amyloid ?? Protein...
Wan Z, Rui L, Li Z. Cell Tissue Res. 2014 May;356(2):341-56. Doi: 10.1007/S00441-014-1804-1. Epub 2014 Apr 2. Expression Patterns Of Prdm1 During Chicken Embryonic And Germline Development.