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SKU AR0155
Pack size 1 kit, enough reagents for 50-100 assays
Kit Components Protein Extraction Solution A 30ml
Protein Extraction Solution B 100ml
Applications Immunoassays: WB, ELISA, RIA, immunoprecipitation; reporter assays; protein kinase assays; protein purifications; folding studies; chromatographic studies; DNA-protein interaction assays
Price: $100.00
Quantity:

Features/Benefits

Boster's Membrane Protein Extraction Kit features:

  • 1. Small scale, simple, reagent based no need for physical, heating procedures. No need for special equipment.
  • 2. Compatible with cultured cells and tissues.
  • 3. Compatible with WB, SDS-page, IP and BCA/protein assays.
  • 4. Procedure takes less than one hour, simple and fast.
  • 5. Low cross contamination <10%

Overview

Product Name Membrane Protein Extraction Kit
Synonyms Membrane Protein solubilizing reagent; Membrane Protein detergent lysis reagent; Membrane Protein Extraction Reagent
Description Membrane Protein Extraction Kit is a ready-to-use Western blot related reagent solution used for efficient extraction of total soluble protein in nondenatured state from animal tissues by homogenization and cell lysis utilizing a dialyzable mild detergent at low concentration, for maximal protein solubilization and minimal interference with protein biological activity enabling direct use in downstream analysis.
Application Immunoassays: WB, ELISA, RIA, immunoprecipitation; reporter assays; protein kinase assays; protein purifications; folding studies; chromatographic studies; DNA-protein interaction assays
Pack Size 1 kit, enough reagents for 50-100 assays
Reagent Type Universal tissue extraction buffer, detergent solution, cell lysis buffer
Kit Components Protein Extraction Solution A 30ml
Protein Extraction Solution B 100ml
Usage Separation of membrane proteins and membrane associated proteins from cultured cells and tissue

Properties

Product Type Ready to use
Form Supplied Solution
Physical State Liquid
Pack Size 1 kit with two components
Reactivity/severeness Bland, minimal amount of detergent, detergent is dialyzable
Enzymatic Activity None; use additives to add function
Additional components compatibility Compatible with additives, e.g. protease inhibitors (PMSF), reducing agents, chelating agents, salts
Downstream methods compatibility Compatible with standard protein detection methods; immunoassays; enzyme assays; common reporter assays
Storage Store at 4˚C for one year.
Precautions FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE

Protocols

Check datasheet for details:

Membrane Protein Extraction Kit Images

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Membrane Protein Extraction Kit
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Publications

Sadam, A., Parida, S., Padol, A.R., Verma, A.D., Baba, N.A., Khuman, W.M.,…, & Sarkar, S.N. (2017). Study of lysophosphatidic acid receptors (LPARs) in buffalo uterus demonstrated upregulation of LPAR1 and LPAR6 in early pregnancy. Theriogenology, 99, 90-97. doi: 10.1016/j.theriogenology.2017.05.019
Padol AR, Sukumaran SV, Sadam A, Kesavan M, Arunvikram K, Verma AD, Srivastava V, Panigrahi M, Singh TU, Telang AG, Mishra SK, Parida S. Reproduction. 2017 May;153(5):565-576. doi: 10.1530/REP-16-0446. Hypercholesterolemia impairs oxytocin-induced uterine contractility in late pregnant mouse.
Zhang W, Zhao G, Hu X, Wang M, Li H, Ye Y, Du Q, Yao J, Bao Z, Hong W, Fu G, Ge J, Qiu Z. J Cell Mol Med. 2014 Jul;18(7):1247-56. Doi: 10.1111/Jcmm.12273. Epub 2014 Apr 6. Aliskiren-Attenuated Myocardium Apoptosis Via Regulation Of Autophagy And C...

FAQs

Q: Can I homogenize my sample in protease inhibitor containing PBS or protein extraction solution A? If PBS can be used, what should be the volume for 100 mg tissue?
A: You can add 150 μL of Protein Extraction Solution A as the homogenization buffer and then incubate it for 10 min after homogenization.