|Pack size||1 kit, sufficient for 50 assays|
|Description||Mitochondria Isolation Kit, Used to isolate the intact and purified mitochondria from animal cells and tissues, and prepare the mitochondria protein of animal tissues and cultured cells.|
Boster's Mitochondria Isolation Kit features:
- 1. Small scale, simple, reagent based no need for physical, heating procedures. No need for special equipment.
- 2. Compatible with cultured cells and tissues.
- 3. Compatible with WB, SDS-page, IP and BCA/protein assays.
- 4. Procedure takes less than one hour, simple and fast.
- 5. Low cross contamination < 10%
The Mitochondria Isolation Kit enables isolation of intact mitochondria from mammalian cells and fresh tissues. Highly purified mitochondria protein will be subsequently yielded after applying lysis buffer containing protease inhibitor. The kit uses traditional Dounce homogenization and the isolation relies on differential centrifugation to separate the mitochondrial and cytosolic fractions with microcentrifuge. Once isolated, the obtained mitochondria can be used for a number of downstream applications, including SDS-PAGE, IP, Western Blotting, Dimensional Electrophoresis, Co-Immunoprecipitation Analysis and Enzymatic Determination.
|Product Name||Mitochondria Isolation Kit|
|Description||Mitochondria Isolation Kit is a ready-to-use Western blot related reagent solution used for efficient isolation of mitochondria from animal homogenized animal tissues and cell lysates utilizing a dialyzable mild detergent at low concentration, for maximal protein solubilization and minimal interference with protein biological activity enabling direct use in downstream analysis.|
|Application||Immunoassays: WB, ELISA, RIA, immunoprecipitation; reporter assays; protein kinase assays; protein purifications; folding studies; chromatographic studies; DNA-protein interaction assays|
|Pack Size||1 kit, enough reagents for 50-100 assays|
|Reagent Type||Universal tissue extraction buffer, detergent solution, cell lysis buffer|
|Kit Components||Mitochondria Isolation Reagent A 100 ml
Mitochondria Isolation Reagent B (Mitochondria lysis buffer) 50 ml
Mitochondria Isolation Reagent C (Trypan Blue Solution) 10ml
|Usage||Separation of Mitochondria from eukaryotic cells|
|Product Type||Ready to use|
|Pack Size||1 kit|
|Reactivity/severeness||Bland, minimal amount of detergent, detergent is dialyzable|
|Enzymatic Activity||None; use additives to add function|
|Additional components compatibility||Compatible with additives, e.g. protease inhibitors (PMSF), reducing agents, chelating agents, salts|
|Downstream methods compatibility||Compatible with standard protein detection methods; immunoassays; enzyme assays; common reporter assays|
|Storage||Store at 4˚C for frequent use, at -20˚C for at least one year.|
|Precautions||FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE|
Mitochondria Isolation Kit Images
Click the images to enlarge.
Integrity of mitochondria extracted from cultured cells
Hela cells were extracted with Boster Mitochondria Isolation Kit using Dounce homogenization. Mitochondrial(Lane 1) and cytosolic fractions( Lane 2) were analyzed via Western blot for Cytochrome C. Results indicate that mitochondria remained intact.