Monkey primate BMP-4 ELISA Kit PicoKine®

BMP-4 ELISA kit for Monkey

Monkey primate BMP-4 ELISA Kit PicoKine™ (96 Tests). Quantitate Monkey BMP4 in cell culture supernatants, cell lysates and serum. Sensitivity: 4pg/ml.

Product Info Summary

SKU: EK0314-PR
Size: 96 wells/kit, with removable strips.
Reactive Species: Monkey
Application: ELISA
Sample Types: cell culture supernatants, cell lysates and serum.

Product Name

Monkey primate BMP-4 ELISA Kit PicoKine®

View all BMP-4 ELISA kits

SKU/Catalog Number

EK0314-PR

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Monkey primate BMP-4 ELISA Kit PicoKine™ (96 Tests). Quantitate Monkey BMP4 in cell culture supernatants, cell lysates and serum. Sensitivity: 4pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Monkey primate BMP-4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0314-PR)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: S293-R408

Sensitivity

<4 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0314-PR for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0314-PR is reactive to BMP4 in Monkey samples

Validated Sample Types

cell culture supernatants, cell lysates and serum.

Application Guarantee

EK0314-PR is guaranteed for ELISA in Monkey by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of BMP-4

Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23.1, The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-beta superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In monkey primate embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. The standard product used in this kit is recombinant BMP-4 with the molecular mass of 26KDa.

Kit Components

Catalog Number Description Quantity
EK0314-PR-CAP Anti-Monkey BMP4 Pre-coated 96-well strip microplate 1
EK0314-PR-ST Monkey BMP4 Standard 2 vials, 10 ng/tube
EK0314-PR-DA Monkey BMP4 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0310.0490.0830.1430.2850.5231.0371.878

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Monkey primate BMP-4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)376987213645312092351
Standard deviation45.40945.40117.4823.1068.91141.06
CV (%)4.24.65.56.05.76.0

Gene/Protein Information For BMP4 (Source: Uniprot.Org, NCBI)

Gene Name

BMP4

Full Name

Bone morphogenetic protein 4

Weight

46.555kDa

Superfamily

TGF-beta family

Alternative Names

BMP2B; BMP-2B; BMP2B1; BMP2BMCOPS6; BMP4; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; MCOPS6; OFC11; ZYME BMP4 BMP2B, BMP2B1, MCOPS6, OFC11, ZYME bone morphogenetic protein 4 bone morphogenetic protein 4|bone morphogenetic protein 2B

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on BMP4, check out the BMP4 Infographic

BMP4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for BMP4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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9 Customer Q&As for Monkey primate BMP-4 ELISA Kit PicoKine®

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-05-24

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-05-24

Question

Q: Can BMP-4 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

M. Johnson

Verified customer

Asked: 2019-10-11

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-10-11

Question

Q: how to thaw whole blood sample for BMP-4 ELISA after freezing?

V. Edwards

Verified customer

Asked: 2019-05-30

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test BMP-4 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2019-05-30

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-04-22

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-04-22

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement BMP-4 using the EK0314-PR Monkey BMP-4 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-03-24

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2019-03-24

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

M. Harris

Verified customer

Asked: 2018-11-08

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-11-08

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

M. King

Verified customer

Asked: 2018-09-17

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-09-17

Question

Q: can you recommend the dilution ratio of serum samples for detection of BMP-4 in Monkey bone tissue? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

W. Roberts

Verified customer

Asked: 2018-01-18

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the BMP-4 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-01-18

Question

Q: how to proceed with the analysis of ELISA data? I have performed BMP-4 level in bone tissue.

Verified Customer

Verified customer

Asked: 2014-06-25

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2014-06-25

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