Mouse Anti-human CD7 Monoclonal Antibody PE Conjugated, Flow Validated

CD7 antibody

Boster Bio Mouse Anti-human CD7 Monoclonal Antibody PE Conjugated, Flow Validated (Catalog# FC01974-1-PE). Tested in Flow Cytometry application(s). This antibody reacts with Human. Cited in 3 publication(s).

Product Info Summary

SKU: FC01974-1-PE
Size: 100 tests, 200 tests
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry

Product Name

Mouse Anti-human CD7 Monoclonal Antibody PE Conjugated, Flow Validated

View all CD7 Antibodies

SKU/Catalog Number

FC01974-1-PE

Size

100 tests, 200 tests

Form

Liquid

Description

Boster Bio Mouse Anti-human CD7 Monoclonal Antibody PE Conjugated, Flow Validated (Catalog# FC01974-1-PE). Tested in Flow Cytometry application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Avoid repeated freeze-thaw cycles.

Cite This Product

Mouse Anti-human CD7 Monoclonal Antibody PE Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC01974-1-PE)

Host

Mouse

Contents

PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide

Clonality

Monoclonal

Clone Number

Clone: 124-1D1

Isotype

IgG1,k

Immunogen

Human Leukocytes

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

FC01974-1-PE is reactive to CD7 in Human

Applications

FC01974-1-PE is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

25.409kDa

Background of CD7

CD7 (Cluster of Differentiation 7) is a protein that in humans is encoded by the CD7 gene. This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

User needs to optimize the dilution ratio for this antibody.

Validation Images & Assay Conditions

Gene/Protein Information For CD7 (Source: Uniprot.org, NCBI)

Gene Name

CD7

Full Name

T-cell antigen CD7

Weight

25.409kDa

Alternative Names

CD7 antigen (p41); CD7 antigen; CD7 molecule; CD7; GP40T-cell surface antigen Leu-9; LEU-9; T-cell antigen CD7; T-cell leukemia antigen; Tp40; TP41; TP41p41 protein CD7 GP40, LEU-9, TP41, Tp40 CD7 molecule T-cell antigen CD7|CD7 antigen (p41)|T-cell leukemia antigen|T-cell surface antigen Leu-9|p41 protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD7, check out the CD7 Infographic

CD7 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

FC01974-1-PE has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Nucleolin promotes Ang II‑induced phenotypic transformation of vascular smooth muscle cells via interaction with tropoelastin mRNA Li Fang 1, Peng-Fei Zhang 2, Kang-Kai Wang 3, Zhi-Lin Xiao 4, Mei Yang 4, Zai-Xin Yu 1 Int J Mol Med. 2019 Apr;43(4):159

Expression of keratin 15 in dentigerous cyst, odontogenic keratocyst and ameloblastoma Mohammed Amjed Alsaegh,et al. Mol Clin Oncol. 2019 Mar; 10(3): 377–381. Published online 2019 Jan 17. doi: 10.3892/mco.2019.1802

Changes in Tumor-Infiltrating Lymphocytes and Vascular Normalization in Breast Cancer Patients After Neoadjuvant Chemotherapy and Their Correlations With DFS Qiong Wang, et al. Front Oncol. 2019; 9: 1545. Published online 2020 Jan 22. doi: 10.3389/fonc

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10 Customer Q&As for Mouse Anti-human CD7 Monoclonal Antibody PE Conjugated, Flow Validated

Question

Is there a BSA free version of Mouse anti-human CD7 Monoclonal antibody PE Conjugated, Flow Validated FC01974-1-PE available?

Verified Customer

Verified customer

Asked: 2020-05-07

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this Mouse anti-human CD7 Monoclonal antibody PE Conjugated, Flow Validated FC01974-1-PE are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-05-07

Question

Is a blocking peptide available for product Mouse anti-human CD7 Monoclonal antibody PE Conjugated, Flow Validated (FC01974-1-PE)?

Verified Customer

Verified customer

Asked: 2020-03-25

Answer

We do provide the blocking peptide for product Mouse anti-human CD7 Monoclonal antibody PE Conjugated, Flow Validated (FC01974-1-PE). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-03-25

Question

I was wanting to use your Mouse anti-human CD7 Monoclonal antibody PE Conjugated, Flow Validated for Flow Cytometry for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?

Verified Customer

Verified customer

Asked: 2020-02-13

Answer

As indicated on the product datasheet, FC01974-1-PE Mouse anti-human CD7 Monoclonal antibody PE Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-13

Question

I have attached the WB image, lot number and protocol we used for blood using Mouse anti-human CD7 Monoclonal antibody PE Conjugated, Flow Validated FC01974-1-PE. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-13

Question

I have a question about product FC01974-1-PE, Mouse anti-human CD7 Monoclonal antibody PE Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-09-18

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC01974-1-PE Mouse anti-human CD7 Monoclonal antibody PE Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-09-18

Question

I am looking for to test Mouse anti-human CD7 Monoclonal antibody PE Conjugated, Flow Validated FC01974-1-PE on human blood for research purposes, then I may be interested in using Mouse anti-human CD7 Monoclonal antibody PE Conjugated, Flow Validated FC01974-1-PE for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-07-22

Answer

The products we sell, including Mouse anti-human CD7 Monoclonal antibody PE Conjugated, Flow Validated FC01974-1-PE, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-07-22

Question

Would FC01974-1-PE Mouse anti-human CD7 Monoclonal antibody PE Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-02-22

Answer

You can see on the product datasheet, FC01974-1-PE Mouse anti-human CD7 Monoclonal antibody PE Conjugated, Flow Validated as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-02-22

Question

I see that the Mouse anti-human CD7 Monoclonal antibody PE Conjugated, Flow Validated FC01974-1-PE works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

H. Banerjee

Verified customer

Asked: 2017-09-12

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-09-12

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using Mouse anti-human CD7 Monoclonal antibody PE Conjugated, Flow Validated FC01974-1-PE. Let me know if you need anything else.

A. Anderson

Verified customer

Asked: 2017-06-09

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-06-09

Question

Will Mouse anti-human CD7 Monoclonal antibody PE Conjugated, Flow Validated FC01974-1-PE work for Flow Cytometry with blood?

Z. Anderson

Verified customer

Asked: 2015-04-22

Answer

According to the expression profile of blood, CD7 is highly expressed in blood. So, it is likely that Mouse anti-human CD7 Monoclonal antibody PE Conjugated, Flow Validated FC01974-1-PE will work for Flow Cytometry with blood.

Boster Scientific Support

Answered: 2015-04-22

Order DetailsPrice
FC01974-1-PE-100tests

100 tests

$290
FC01974-1-PE-200tests

200 tests

$467

Free Secondary Antibody

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FC01974-1-PE
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$290.00

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