Mouse ANTXR2 ELISA Kit PicoKine®

CMG-2/ANTXR2 ELISA kit for Mouse

Mouse ANTXR2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse ANTXR2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK2122
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Mouse ANTXR2 ELISA Kit PicoKine®

View all CMG-2/ANTXR2 ELISA kits

SKU/Catalog Number

EK2122

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse ANTXR2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse ANTXR2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse ANTXR2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2122)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q32-G318

Sensitivity

<50 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2122 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2122 is reactive to Antxr2 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK2122 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CMG-2/ANTXR2

Anthrax toxin receptor 2 is a protein that in humans is encoded by the ANTXR2 gene. It is mapped to 5; 5 E3. This gene encodes a receptor for anthrax toxin. The protein binds to collagen IV and laminin, suggesting that it may be involved in extracellular matrix adhesion. Mutations in this gene cause juvenile hyaline fibromatosis and infantile systemic hyalinosis. Multiple transcript variants encoding different isoforms have been found for this gene.

Kit Components

Catalog Number Description Quantity
EK2122-CAP Anti-Mouse Antxr2 Pre-coated 96-well strip microplate 1
EK2122-ST Mouse Antxr2 Standard 2 vials, 10 ng/tube
EK2122-DA Mouse Antxr2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0150.0760.1340.2390.5090.9041.4572.265

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse ANTXR2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2421644563121717715410
Standard deviation14.52118.36360.3814.53146.99394.93
CV (%)6%7.2%6.4%6.7%8.3%7.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CMG-2/ANTXR2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12422472462422442.270.9%
Sample 216441884178816011729113.046.5%
Sample 356315520514549735317268.195%
*number of samples for each test n=16.

Gene/Protein Information For Antxr2 (Source: Uniprot.Org, NCBI)

Gene Name

Antxr2

Full Name

Anthrax toxin receptor 2

Weight

53.184kDa

Superfamily

ATR family

Alternative Names

anthrax toxin receptor 2; ANTXR2; Capillary morphogenesis gene 2 protein; capillary morphogenesis protein 2; cI-35; CMG2; CMG-2; CMG2MGC111533; CMG-2MGC45856; FLJ31074; ISH; JHF; JHS Antxr2|2310046B19Rik, AW561899, CMG-2, CMG2, cI-3, cI-35|anthrax toxin receptor 2|anthrax toxin receptor 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Antxr2, check out the Antxr2 Infographic

Antxr2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Antxr2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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5 Customer Q&As for Mouse ANTXR2 ELISA Kit PicoKine®

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-06-09

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-06-09

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring CMG-2 using the EK2122 Mouse CMG-2 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-03-26

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2020-03-26

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

T. Bhatt

Verified customer

Asked: 2019-08-05

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-08-05

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

J. Smith

Verified customer

Asked: 2016-06-21

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2016-06-21

Question

Q: how can I thaw whole blood sample for CMG-2 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2014-06-03

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CMG-2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2014-06-03

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