Mouse beta IG-H3/TGFBI ELISA Kit PicoKine®

Beta Ig-h3/TGFBI ELISA kit for Mouse

Mouse beta IG-H3/TGFBI ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tgfbi in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK1191
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse beta IG-H3/TGFBI ELISA Kit PicoKine®

View all Beta Ig-h3/TGFBI ELISA kits

SKU/Catalog Number

EK1191

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse beta IG-H3/TGFBI ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tgfbi in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse beta IG-H3/TGFBI ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1191)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: G24-H683

Sensitivity

<5 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1191 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1191 is reactive to TGFBI in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1191 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Beta Ig-h3/TGFBI

Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.

Kit Components

Catalog Number Description Quantity
EK1191-CAP Anti-Mouse TGFBI Pre-coated 96-well strip microplate 1
EK1191-ST Mouse TGFBI Standard 2 vials, 10 ng/tube
EK1191-DA Mouse TGFBI Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0040.0360.0710.1470.3210.7331.3732.009

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Beta Ig-h3/TGFBI, Dilution ratio of 1:200, concentration in serum and plasma is 200-626 ng/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse beta IG-H3/TGFBI ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)30115302548230215765124
Standard deviation21.3795.7241.222.65100.86312.56
CV (%)7.1%6%4.4%7.5%6.4%6.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Beta Ig-h3/TGFBI in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 130131532929030814.664.7%
Sample 21595147514671429149162.234.1%
Sample 354826340619162426063340.115.6%
*number of samples for each test n=16.

Gene/Protein Information For TGFBI (Source: Uniprot.Org, NCBI)

Gene Name

TGFBI

Full Name

Transforming growth factor-beta-induced protein ig-h3

Weight

74.681kDa

Alternative Names

beta IGH3; beta IG-H3; BIGH3EBMD; CDB1; CDG2; CDGG1transforming growth factor, beta-induced, 68kD; CSD; CSD1; CSD2; CSD3; Kerato-epithelin; LCD1; RGD-CAP; RGD-containing collagen-associated protein; TGFBI; TGFBIP; transforming growth factor, beta-induced, 68kDa; transforming growth factor-beta-induced protein ig-h3 TGFBI BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, LCD1 transforming growth factor beta induced transforming growth factor-beta-induced protein ig-h3|RGD-CAP|RGD-containing collagen-associated protein|beta ig-h3|betaig-h3|kerato-epithelin|transforming growth factor beta-induced 68kDa|transforming growth factor, beta-induced, 68kD

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TGFBI, check out the TGFBI Infographic

TGFBI infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TGFBI: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK1191 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Characterization of bone marrow-derived mesenchymal stem cells from dimethyloxallyl glycine-preconditioned mice: Evaluation of the feasibility of dimethyloxallyl glycine as a mobilization agent

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7 Customer Q&As for Mouse beta IG-H3/TGFBI ELISA Kit PicoKine®

Question

Does EK1191 contain native human biological materials?

Verified customer

Asked: 2021-11-10

Answer

The Mouse Beta IG-H3/TGFBI ELISA Kit PicoKine® (EK1191) doesn't contain native human biological materials. doesn't contain native human biological materials.

Boster Scientific Support

Answered: 2021-11-11

Question

Q: Can Beta Ig-h3 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-05-28

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-05-28

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-02-29

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-02-29

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-09-03

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-09-03

Question

Q: how to proceed with the analysis of ELISA data? I have obtained Beta Ig-h3 level in serum.

Verified Customer

Verified customer

Asked: 2017-11-25

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2017-11-25

Question

Q: how to thaw whole blood sample for Beta Ig-h3 ELISA after freezing?

W. White

Verified customer

Asked: 2017-02-25

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Beta Ig-h3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2017-02-25

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

D. Mangal

Verified customer

Asked: 2014-08-11

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2014-08-11

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