Mouse Crossveinless-2/CV-2/BMPER ELISA Kit PicoKine®

Crossveinless-2/CV-2/BMPER ELISA kit for Mouse

Mouse Crossveinless-2/CV-2/BMPER ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Bmper in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK2119
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Mouse Crossveinless-2/CV-2/BMPER ELISA Kit PicoKine®

View all Crossveinless-2/CV-2/BMPER ELISA kits

SKU/Catalog Number

EK2119

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Crossveinless-2/CV-2/BMPER ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Bmper in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse Crossveinless-2/CV-2/BMPER ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2119)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: V34-R685

Sensitivity

<50 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2119 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2119 is reactive to Bmper in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK2119 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Crossveinless-2/CV-2/BMPER

This gene encodes a secreted protein that interacts with, and inhibits bone morphogenetic protein (BMP) function. It has been shown to inhibit BMP2- and BMP4-dependent osteoblast differentiation and BMP-dependent differentiation of the chondrogenic cells. Mutations in this gene are associated with a lethal skeletal disorder, diaphanospondylodysostosis.

Kit Components

Catalog Number Description Quantity
EK2119-CAP Anti-Mouse Bmper Pre-coated 96-well strip microplate 1
EK2119-ST Mouse Bmper Standard 2 vials, 10 ng/tube
EK2119-DA Mouse Bmper Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0590.1430.1930.3070.5620.9171.4522.098

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Crossveinless-2/CV-2/BMPER, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 62.5 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse Crossveinless-2/CV-2/BMPER ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)20781314592128461558
Standard deviation11.1846.3497.7512.3062.60123.08
CV (%)5.4%5.7%6.7%5.8%7.4%7.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Crossveinless-2/CV-2/BMPER in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12072202151952097.943.8%
Sample 281382785479282246.855.7%
Sample 31459132214781334139886.686.2%
*number of samples for each test n=16.

Gene/Protein Information For Bmper (Source: Uniprot.Org, NCBI)

Gene Name

Bmper

Full Name

BMP-binding endothelial regulator protein

Weight

76.148kDa

Alternative Names

BMP binding endothelial regulator; BMP-binding endothelial regulator precursor protein; BMP-binding endothelial regulator protein; BMPER; Bone morphogenetic protein-binding endothelial cell precursor-derived regulator; CRIM3; crossveinless 2; Crossveinless-2; CV2; CV-2; hCV2; KIAA1965; Protein crossveinless-2 Bmper|3110056H04Rik, CV-, CV-2, Cri, Crim3, Cv, Cv2|BMP-binding endothelial regulator|BMP-binding endothelial regulator protein|bone morphogenetic protein-binding endothelial cell precursor-derived regulator|crossveinless-2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Bmper, check out the Bmper Infographic

Bmper infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Bmper: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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7 Customer Q&As for Mouse Crossveinless-2/CV-2/BMPER ELISA Kit PicoKine®

Question

Does EK2119 contain native human biological materials?

Verified customer

Asked: 2021-11-10

Answer

The Mouse Crossveinless-2/CV-2/BMPER ELISA Kit PicoKine® (EK2119) doesn't contain native human biological materials.

Boster Scientific Support

Answered: 2021-11-11

Question

Q: how to proceed with the analysis of ELISA data? I measured BMPER level in serum.

Verified Customer

Verified customer

Asked: 2020-09-03

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-09-03

Question

Q: how to thaw whole blood sample for BMPER ELISA after freezing?

B. White

Verified customer

Asked: 2020-01-04

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test BMPER for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-01-04

Question

Q: can you recommend the dilution ratio of serum samples for detection of BMPER in Mouse serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2019-10-14

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the BMPER ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-10-14

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

J. Carter

Verified customer

Asked: 2019-06-10

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-06-10

Question

Q: Can BMPER ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

N. Anand

Verified customer

Asked: 2016-08-26

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2016-08-26

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

W. Zhang

Verified customer

Asked: 2014-10-08

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2014-10-08

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