Mouse CCL9/Mip 1 Gamma PicoKine™ Fast ELISA Kit
The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Mouse Mip 1 Gamma/Ccl9 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Mip 1 Gamma/Ccl9 tissue specificity: Expressed mainly in the liver, lung, and the thymus, although some expression has been The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detected in a wide variety of tissues except brain.
Mouse CCL9/Mip 1 Gamma PicoKine™ Fast ELISA Kit Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
Boster's Picokine™ Fast ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
|Product Name||Mouse CCL9/Mip 1 Gamma PicoKine™ Fast ELISA Kit
See all Ccl9 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Mouse Mip 1 Gamma/Ccl9 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Mip 1 Gamma/Ccl9 tissue specificity: Expressed mainly in the liver, lung, and the thymus, although some expression has been The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detected in a wide variety of tissues except brain.|
|Cite This Product||Mouse CCL9/Mip 1 Gamma PicoKine™ Fast ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1225)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen||Expression system for standard: E.coli; Immunogen sequence: Q22-Q122|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Protein Target Info (Source: Uniprot.org)
|Protein Name||C-C motif chemokine 9|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Monokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors.|
|Background||Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone.|
Kit Components And Assay QC Data Details Of Mouse CCL9/Mip 1 Gamma PicoKine™ Fast ELISA Kit
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|FEK1225-CAP||96-well plate precoated with anti-Mouse Ccl9 antibody||1|
|FEK1225-ST||lyophilized recombinant Mouse Ccl9 standard||10ng/tube|
|FEK1225-DA||biotinylated anti-Mouse Ccl9 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of FEK1225 Mouse CCL9/Mip 1 Gamma PicoKine™ Fast ELISA Kit. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-20min
We measured random samples of Mouse CCL9/Mip 1 Gamma PicoKine™ Fast ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Mouse CCL9/Mip 1 Gamma PicoKine™ Fast ELISA Kit to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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