Mouse CD5L/CT-2 PicoKine® Quick ELISA Kit

CD5L ELISA kit, 90-minute one-step protocol

Mouse CD5L / CT 2 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Cd5l in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: FEK1414
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Assay Time: 90 Minutes

How Quick Picokine(R) ELISA kits work: Get data in 90 minutes

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Product Name

Mouse CD5L/CT-2 PicoKine® Quick ELISA Kit

See all CD5L products

SKU/Catalog Number

FEK1414

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse CD5L / CT 2 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Cd5l in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse CD5L/CT-2 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1414)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E22-V352

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK1414 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1414 is reactive to CD5L in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

FEK1414 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CD5L

CD5 antigen-like, also known as Sp alpha and AIM, is a protein that in humans is encoded by the CD5L gene. It is mapped to 1q21-q23 by fluorescence in situ hybridization. It is found that Aim expression is induced in mouse macrophages in response to loading with highly oxidized low density lipoprotein (oxLDL), and that Aim is expressed in foam cells within atherosclerotic lesions. Both the expression of Aim in lesions and its induction by oxLDL require Lxr /Rxr heterodimers. Aim-null macrophages are highly susceptible to oxLDL-induced apoptosis in vitro and undergo accelerated apoptosis in atherosclerotic lesions in vivo. Double knockout of Aim and Ldlr reduce atherosclerotic lesions. Therefore, it is concluded that AIM expression protects macrophages from apoptosis within atherosclerotic lesions, promoting early lesion development.

Kit Components

Catalog numberDescription QuantityVolume
FEK1414-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
EK1414-STMouse CD5L Standard 2 10 ng/tube
FEK1414-AMouse CD5L Antibody A 1 3ml
FEK1414-BMouse CD5L Antibody B 1 3ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)01563126251,2502,5005,00010,000
O.D.0.0040.1310.2090.4240.7471.2941.9962.436

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2011789494020719354821
Standard deviation13.26110.91375.4417.38131.58414.6
CV (%)6.6%6.2%7.6%8.4%6.8%8.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CD5L in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 120123623222222213.556.1%
Sample 21789179518751980185977.284.1%
Sample 349404445482548344761187.963.9%
*number of samples for each test n=16.

Gene/Protein Information For CD5L (Source: Uniprot.Org, NCBI)

Gene Name

CD5L

Full Name

CD5 antigen-like

Weight

38.088kDa

Alternative Names

AIM; API6; apoptosis inhibitor 6; CD5 antigen-like (scavenger receptor cysteine rich family); CD5 antigen-like; CD5 molecule-like; CD5L; CT-2; IgM-associated peptide; PRO229; Spalpha; SP-alpha CD5L AIM, API6, CT-2, PRO229, SP-ALPHA, Spalpha, hAIM CD5 molecule like CD5 antigen-like|CD5 antigen-like (scavenger receptor cysteine rich family)|apoptosis inhibitor 6|apoptosis inhibitor expressed by macrophages|apoptosis inhibitor of macrophage|igM-associated peptide

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD5L, check out the CD5L Infographic

CD5L infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD5L: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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