Mouse CRLF2/TSLP R ELISA Kit PicoKine®

TSLPR/CRLF2 ELISA kit for Mouse

Mouse CRLF2/TSLP R ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Crlf2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1665
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Mouse CRLF2/TSLP R ELISA Kit PicoKine®

View all TSLPR/CRLF2 ELISA kits

SKU/Catalog Number

EK1665

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse CRLF2/TSLP R ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Crlf2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse CRLF2/TSLP R ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1665)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A20-L233

Sensitivity

<10 pg/ml

Assay Range

125 pg/ml - 8,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1665 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1665 is reactive to Crlf2 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1665 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of TSLPR/CRLF2

Cytokine receptor-like factor 2, also known as TSLPR or CRL2, is a protein that in humans is encoded by the CRLF2 gene. It is mapped to Xp22.33. The protein encoded by this gene is a receptor for thymic stromal lymphopoietin (TSLP). Together with the interleukin 7 receptor (IL7R), the encoded protein and TSLP activate STAT3, STAT5, and JAK2 pathways, which control processes such as cell proliferation and development of the hematopoietic system. Two transcript variants encoding different isoforms have been found for this gene. In addition to it, it has been found that rearrangement of CRLF2 and JAK mutation together contribute to leukemogenesis in B-progenitor ALL.

Kit Components

Catalog Number Description Quantity
EK1665-CAP Anti-Mouse Crlf2 Pre-coated 96-well strip microplate 1
EK1665-ST Mouse Crlf2 Standard 2 vials, 10 ng/tube
EK1665-DA Mouse Crlf2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)01252505001000200040008000
O.D.0.0520.1060.1590.2490.4380.7811.5152.342

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TSLPR/CRLF2, Dilution ratio of 1:1, concentration in serum and plasma is around 400 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse CRLF2/TSLP R ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)15596261421509026620
Standard deviation9.7674.07405.3711.8571.25450.16
CV (%)7.9%7.7%6.6%7.9%7.9%6.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TSLPR/CRLF2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 115518018215616812.777.6%
Sample 2962986889968951373.8%
Sample 361425679544261375850301.385.1%
*number of samples for each test n=16.

Gene/Protein Information For Crlf2 (Source: Uniprot.Org, NCBI)

Gene Name

Crlf2

Full Name

Cytokine receptor-like factor 2

Weight

37.762kDa

Superfamily

type I cytokine receptor family

Alternative Names

CRL2; CRL2cytokine receptor CRL2 precusor; CRLF2; CRLF2Y; CRLM-2; Cytokine receptor-like 2; cytokine receptor-like factor 2; ILXR; IL-XR; P2RY8/CRLF2 fusion; PCOR1; Thymic stromal lymphopoietin protein receptor; thymic stromal lymphopoietin receptor; thymic stromal-derived lymphopoietin receptor; TSLP R; TSLP receptor; TSLPR Crlf2|CRLM2, Ly11, Ly114, T, Tpte, Tpte2, Tslpr|cytokine receptor-like factor 2|cytokine receptor-like factor 2|TSLP receptor|cytokine receptor-like molecule 2|lymphocyte antigen 114|thymic stromal lymphopoietin protein receptor|thymic stromal-derived lymphopoietin, receptor|transmembrane phosphoinositide 3-phosphatase and tensin homolog 2|type I cytokine receptor delta 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Crlf2, check out the Crlf2 Infographic

Crlf2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Crlf2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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11 Customer Q&As for Mouse CRLF2/TSLP R ELISA Kit PicoKine®

Question

Does EK1665 contain native human biological materials?

Verified customer

Asked: 2021-11-10

Answer

The Mouse CRLF2/TSLP R ELISA Kit PicoKine® (EK1665) doesn't contain native human biological materials. doesn't contain native human biological materials.

Boster Scientific Support

Answered: 2021-11-10

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-09-13

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-09-13

Question

Q: can you recommend the dilution ratio of serum samples for detection of TSLPR in Mouse plasma? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2020-05-18

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TSLPR ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-05-18

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring TSLPR using the EK1665 Mouse TSLPR Picokine® ELISA Kit?

H. Walker

Verified customer

Asked: 2019-12-23

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2019-12-23

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-11-24

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-11-24

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-09-25

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-09-25

Question

Is EK1665 kit recommended for rat samples?

Verified customer

Asked: 2019-09-11

Answer

The Mouse CRLF2/TSLP R ELISA Kit PicoKine EK1665 is not recommended for rat samples. It is specific for mouse samples.

Boster Scientific Support

Answered: 2019-09-11

Question

Our customer wonder whether Mouse CRLF2/TSLP R PicoKine

Verified Customer

Verified customer

Asked: 2019-07-08

Answer

EK1665 is not recommended to work on rat samples. It is specific for mouse samples.

Boster Scientific Support

Answered: 2019-07-08

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-08-17

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-08-17

Question

Q: Can TSLPR ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2017-11-24

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2017-11-24

Question

Q: which procedure should I follow in order to thaw whole blood sample for TSLPR ELISA after freezing?

Verified Customer

Verified customer

Asked: 2016-09-24

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TSLPR for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2016-09-24

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