Mouse CXCL9/Mig ELISA Kit PicoKine®

CXCL9/MIG ELISA kit for Mouse

Mouse CXCL9/Mig ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cxcl9 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0733
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (EDTA).

Product Name

Mouse CXCL9/Mig ELISA Kit PicoKine®

View all CXCL9/MIG ELISA kits

SKU/Catalog Number

EK0733

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse CXCL9/Mig ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cxcl9 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse CXCL9/Mig ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0733)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: T22-T126

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0733 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0733 is reactive to CXCL9 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (EDTA).

Application Guarantee

EK0733 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CXCL9/MIG

Chemokine (C-X-C motif) ligand 9 (CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon (MIG). CXCL9 is a T-cell chemoattractant, which is induced by IFN-γ. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4. CXCL9, CXCL10 and CXCL11 all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. The standard product used in this kit is recombinant human CXCL9, consisting of 103 amino acids with the molecular mass of 11.7KDa.

Kit Components

Catalog Number Description Quantity
EK0733-CAP Anti-Mouse CXCL9 Pre-coated 96-well strip microplate 1
EK0733-ST Mouse CXCL9 Standard 2 vials, 10 ng/tube
EK0733-DA Mouse CXCL9 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0120.0310.0740.1570.3050.5871.0321.901

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect CXCL9/MIG, Dilution ratio of 1:1, concentration in serum and plasma is 0-710g/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse CXCL9/Mig ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)8622889179240896
Standard deviation4.913.4538.314.7417.2843
CV (%)5.7%4.3%%4.3%6%7.2%4.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CXCL9/MIG in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 186849282863.744.3%
Sample 22282072172122167.773.5%
Sample 389189183087987225.162.8%
*number of samples for each test n=16.

Gene/Protein Information For CXCL9 (Source: Uniprot.Org, NCBI)

Gene Name

CXCL9

Full Name

C-X-C motif chemokine 9

Weight

14.019kDa

Superfamily

intercrine alpha (chemokine CxC) family

Alternative Names

chemokine (C-X-C motif) ligand 9; CMK; crg-10; C-X-C motif chemokine 9; CXCL9; Gamma-interferon-induced monokine; Humig; MIG; MIGSmall-inducible cytokine B9; monokine induced by gamma interferon; SCYB9Monokine induced by interferon-gamma CXCL9 CMK, Humig, MIG, SCYB9, crg-10 C-X-C motif chemokine ligand 9 C-X-C motif chemokine 9|chemokine (C-X-C motif) ligand 9|gamma-interferon-induced monokine|monokine induced by gamma interferon|monokine induced by interferon-gamma|small-inducible cytokine B9

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CXCL9, check out the CXCL9 Infographic

CXCL9 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CXCL9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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6 Customer Q&As for Mouse CXCL9/Mig ELISA Kit PicoKine®

Question

Does EK0733 contain native human biological materials?

Verified customer

Asked: 2021-11-10

Answer

The Mouse CXCL9/Mig ELISA Kit PicoKine® (EK0733) doesn't contain native human biological materials.

Boster Scientific Support

Answered: 2021-11-11

Question

Q: how to proceed with the analysis of ELISA data? I have performed CXCL9 level in serum.

Verified Customer

Verified customer

Asked: 2020-09-20

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-09-20

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-10-09

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-10-09

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

M. Jackson

Verified customer

Asked: 2019-05-02

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-05-02

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CXCL9 in Mouse serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-12-01

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CXCL9 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-12-01

Question

Q: is it okay to use edta plasma as samples in Mouse CXCL9 Picokine® ELISA Kit (Catalog # EK0733)?

Verified Customer

Verified customer

Asked: 2018-05-12

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of CXCL9 causing disruption of its protein structure. CXCL9 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CXCL9 ELISA, treating samples with various anticoagulants and decided that EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-05-12

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