Mouse ERAP1 ELISA Kit PicoKine®

Aminopeptidase PILS/ARTS1 ELISA kit for Mouse

Mouse ERAP1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Erap1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK2028
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Mouse ERAP1 ELISA Kit PicoKine®

View all Aminopeptidase PILS/ARTS1 ELISA kits

SKU/Catalog Number

EK2028

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse ERAP1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Erap1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse ERAP1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2028)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-L930

Sensitivity

<50 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2028 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2028 is reactive to ERAP1 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK2028 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Aminopeptidase PILS/ARTS1

Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator, also known as endoplasmic reticulum aminopeptidase 1 (ARTS-1), is a protein which in humans is encoded by the ARTS-1 gene. This gene is mapped to 13; 13 C1. Endoplasmic reticulum amino peptidase 1 is active in the endoplasmic reticulum, which is involved in protein processing and transport. This protein is an aminopeptidase, which is an enzyme that cleaves peptides at the N-terminal into smaller fragments called amino acids.

Kit Components

Catalog Number Description Quantity
EK2028-CAP Anti-Mouse ERAP1 Pre-coated 96-well strip microplate 1
EK2028-ST Mouse ERAP1 Standard 2 vials, 20 ng/tube
EK2028-DA Mouse ERAP1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03126251250250050001000020000
O.D.0.0120.0660.0890.1810.3650.5781.0911.805

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Aminopeptidase PILS/ARTS1, Dilution ratio of 1:100, concentration in serum and plasma is 0.25-1.3 ug/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse ERAP1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5051975896320585879289
Standard deviation29.29118.50412.30125.5440.50678.10
CV (%)5.8%6.0%4.6%6.1%6.9%7.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Aminopeptidase PILS/ARTS1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 150557852460455239.907.2%
Sample 21975209619181952198567.073.3%
Sample 3896310445939097199629541.905.6%
*number of samples for each test n=16.

Gene/Protein Information For ERAP1 (Source: Uniprot.Org, NCBI)

Gene Name

ERAP1

Full Name

Endoplasmic reticulum aminopeptidase 1

Weight

107.235kDa

Superfamily

peptidase M1 family

Alternative Names

Adipocyte-derived leucine aminopeptidase; A-LAP; A-LAPALAP; Aminopeptidase PILS; ARTS1; ARTS1aminopeptidase regulator of TNFR1 shedding; ARTS-1PILSAP; EC 3.4.11; EC 3.4.11.-; EC 3.4.11.1; endoplasmic reticulum aminopeptidase 1; endoplasmic reticulum aminopeptidase associated with antigen processing; ERAAP1; ERAP1; KIAA0525APPILS; PILS-AP; PILS-APERAAP; Puromycin-insensitive leucyl-specific aminopeptidase; Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator ERAP1 A-LAP, ALAP, APPILS, ARTS-1, ARTS1, ERAAP, ERAAP1, PILS-AP, PILSAP endoplasmic reticulum aminopeptidase 1 endoplasmic reticulum aminopeptidase 1|CTD-2260A17.2|adipocyte-derived leucine aminopeptidase|aminopeptidase PILS|aminopeptidase regulator of TNFR1 shedding|endoplasmic reticulum aminopeptidase associated with antigen processing|puromycin-insensitive leucyl-specific aminopeptidase|type 1 tumor necrosis factor receptor shedding aminopeptidase regulator

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ERAP1, check out the ERAP1 Infographic

ERAP1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ERAP1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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8 Customer Q&As for Mouse ERAP1 ELISA Kit PicoKine®

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Aminopeptidase PILS using the EK2028 Mouse Aminopeptidase PILS Picokine® ELISA Kit?

W. Chakrabarti

Verified customer

Asked: 2020-02-25

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2020-02-25

Question

Q: if the enzyme conjugated Aminopeptidase PILS antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Aminopeptidase PILS antigen?

Verified Customer

Verified customer

Asked: 2019-12-25

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-12-25

Question

Q: Can Aminopeptidase PILS ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-09-11

Question

Q: which procedure should I follow in order to thaw whole blood sample for Aminopeptidase PILS ELISA after freezing?

B. Chakrabarti

Verified customer

Asked: 2019-06-22

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Aminopeptidase PILS for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2019-06-22

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-05-11

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-05-11

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

E. Lewis

Verified customer

Asked: 2019-03-24

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-03-24

Question

Q: how to analyze ELISA data? I have performed Aminopeptidase PILS level in plasma.

Verified Customer

Verified customer

Asked: 2018-08-22

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-08-22

Question

Q: What is the optimal O.D. value for Aminopeptidase PILS ELISA kit? I used your Aminopeptidase PILS ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Aminopeptidase PILS even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-05-13

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Aminopeptidase PILS ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of Aminopeptidase PILS that can be declared/interpreted as positive by the above standard is 50pg/ml.

Boster Scientific Support

Answered: 2018-05-13

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