|Sample Type:||cell culture supernates, serum and plasma(heparin, EDTA, citrate).|
Data & Images
|Product Name||Mouse Fibronectin PicoKine™ ELISA Kit|
|Description||Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Fibronectin|
|Cite This Product||Mouse Fibronectin PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0351)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Immunogen||Expression system for standard: from plasma|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
|Sample Type||cell culture supernates, serum and plasma(heparin, EDTA, citrate).
*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine™ ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
|Capture Antibody||monoclonal antibody from rat|
|Detection Antibody||polyclonal antibody from goat|
|Storage||Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|-||96-well plate precoated with anti- mouse Fibronectin antibody||1|
|ST0000-10||lyophilized natural mouse Fibronectin standard||10ng/tubex2|
|AR1107||biotinylated anti- mouse Fibronectin antibody||130ul(dilution 1:100)|
|AR1103||Avidin-Biotin-Peroxidase Complex(ABC)||130ul(dilution 1:100)|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
*Additional components can be purchased. If you need extra of the above components please order them together to avoid additional shipping charges.See Prices For Extra Components
Material Required But Not Provided
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 4. Clean tubes and Eppendorf tubes.
- 5. Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Gene Full Name||Integrin beta-1|
|Protein Function||Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylatedsequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors forfibronectin. Alpha-4/beta-1 recognizes one or more domains withinthe alternatively spliced CS-1 and CS-5 regions of fibronectin.Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrinalpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1are receptors for lamimin. Integrin alpha-4/beta-1 is a receptorfor VCAM1 and recognizes the sequence Q-I-D-S in VCAM1. Integrinalpha-9/beta-1 is a receptor for VCAM1, cytotactin andosteopontin. It recognizes the sequence A-E-I-D-G-I-E-L incytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin,thrombospondin and CSPG4. Integrin alpha-3/beta-1 provides adocking site for FAP (seprase) at invadopodia plasma membranes ina collagen-dependent manner and hence may participate in theadhesion, formation of invadopodia and matrix degradationprocesses, promoting cell invasion. Alpha-3/beta-1 may mediatewith LGALS3 the stimulation by CSPG4 of endothelial cellsmigration. Integrin alpha-V/beta-1 is a receptor for vitronectin.Beta-1 integrins recognize the sequence R-G-D in a wide array ofligands. When associated with alpha-7/beta-1 integrin, regulatescell adhesion and laminin matrix deposition. Involved in promotingendothelial cell motility and angiogenesis. Involved in osteoblastcompaction through the fibronectin fibrillogenesis cell-mediatedmatrix assembly process and the formation of mineralized bonenodules. May be involved in up-regulation of the activity ofkinases such as PKC via binding to KRT1. Together with KRT1 andGNB2L1, serves as a platform for SRC activation or inactivation.Plays a mechanistic adhesive role during telophase, required forthe successful completion of cytokinesis..|
|Tissue Specificity||Isoform 2 is expressed in skeletal and cardiacmuscles only (at protein level). Isoform 1 is very weaklyexpressed in striated muscles and not detected in adult skeletalmuscle fibers and cardiomyocytes..|
|Sequence Similarities||Belongs to the integrin beta chain family.|
|Subcellular Localization||Cell membrane; Colocalizes with ITGB1BP1 and metastatic suppressor proteinNME2 at the edge or peripheral ruffles and lamellipodia during theearly stages of cell spreading on fibronectin or collagen.Translocates from peripheral focal adhesions to fibrillaradhesions in an ITGB1BP1-dependent manner. Enriched preferentiallyat invadopodia, cell membrane protrusions that correspond to sitesof cell invasion, in a collagen-dependent manner. Localized atplasma and ruffle membranes in a collagen-independent manner..|
|Uniprot ID||Itgb1: P09055
|Alternative Names||Integrin beta-1; Fibronectin receptor subunit beta; VLA-4 subunit beta; CD29; Itgb1;|
Background for Fibronectin
Mouse Fibronectin PicoKine™ ELISA Kit Images
Click the images to enlarge.
Intra/Inter Assay Precision
|Intra-Assay Precision||Inter-Assay Precision|
Typical Data Obtained from Mouse Fibronectin PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 20-25min)
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.