Product Info Summary
SKU: | EK0445 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Mouse |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum and plasma (EDTA). |
Product info
Product Name
Mouse M-CSF ELISA Kit PicoKine®
SKU/Catalog Number
EK0445
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse M-CSF ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Csf1 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse M-CSF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0445)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: K33-E262
Sensitivity
<1 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0445 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0445 is reactive to CSF1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (EDTA).
Application Guarantee
EK0445 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of M-CSF
M-CSF, also called CSF1, consists of a 14-amino acid peptide, longer than the usual 8-to-11 mer recognized by most CTLs. The M-CSF gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb1. Although it is a single-copy gene, its expression results in the synthesis of several mRNAs, ranging in size from about 1.5 to 4.5 kb2. There are 2 forms of M-CSF, with 224 and 522 amino acids, resulting from alternative splicing3. Furthermore, M-CSF has a role in development of the placenta. Uterine M-CSF concentration is regulated by a synergistic action of estradiol and progesterone. M-CSF is produced by uterine glandular epithelial cells. It had been found that FMS, the M-CSF receptor, is expressed in placenta and choriocarcinoma cell lines4.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK0445-CAP | Anti-Mouse CSF1 Pre-coated 96-well strip microplate | 1 |
EK0445-ST | Mouse CSF1 Standard | 2 vials, 10 ng/tube |
EK0445-DA | Mouse CSF1 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.028 | 0.096 | 0.156 | 0.239 | 0.343 | 0.650 | 1.278 | 1.868 |
Data Example Images
Click image to see more details
Mouse M-CSF PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect M-CSF, For serum, dilution ratio is 1:4 and concentration is 510-1678 pg/ml.
For plasma, dilution ratio is 1:4 and concentration is 428-1191 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse M-CSF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 40 | 160 | 459 | 36 | 158 | 501 |
Standard deviation | 1.68 | 11.2 | 21.57 | 1.9 | 13.58 | 30.56 |
CV (%) | 4.2% | 7% | 4.7% | 5.3% | 8.6% | 6.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of M-CSF in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 40 | 41 | 38 | 35 | 38 | 2.29 | 6% |
Sample 2 | 160 | 159 | 164 | 144 | 156 | 7.59 | 4.8% |
Sample 3 | 459 | 393 | 401 | 415 | 417 | 25.49 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For CSF1 (Source: Uniprot.Org, NCBI)
Gene Name
CSF1
Full Name
Macrophage colony-stimulating factor 1
Weight
60.179kDa
Alternative Names
colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; MCSF; M-CSF; MCSFlanimostim; MGC31930 CSF1 CSF-1, MCSF colony stimulating factor 1 macrophage colony-stimulating factor 1|colony stimulating factor 1 (macrophage)|lanimostim|macrophage colony stimulating factor 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CSF1, check out the CSF1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CSF1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse M-CSF ELISA Kit PicoKine® (EK0445)
Hello CJ!
EK0445 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The Involvement of Macrophage Colony Stimulating Factor on Protein Hydrolysate Injection Mediated Hematopoietic Function Improvement
Dmrt1 regulates the immune response by repressing the TLR4 signaling pathway in goat male germline stem cells
Loss of the androgen receptor suppresses intrarenal calcium oxalate crystals deposition via altering macrophage recruitment/M2 polarization with change of the miR-185-5p/CSF-1 signals
Sleep modulates haematopoiesis and protects against atherosclerosis
Species: Mouse
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
7 Customer Q&As for Mouse M-CSF ELISA Kit PicoKine®
Question
Q: Can M-CSF ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-11-18
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-11-18
Question
Q: can you suggest the dilution ratio of serum samples for detection of M-CSF in Mouse plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the M-CSF ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-08-29
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
P. Huang
Verified customer
Asked: 2018-11-18
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-11-18
Question
Q: if the enzyme conjugated M-CSF antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the M-CSF antigen?
L. Wang
Verified customer
Asked: 2018-03-14
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-03-14
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
H. Parker
Verified customer
Asked: 2017-10-17
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-10-17
Question
Q: how to thaw whole blood sample for M-CSF ELISA after freezing?
Verified Customer
Verified customer
Asked: 2016-07-03
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test M-CSF for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2016-07-03
Question
Q: how to analyze ELISA data? I measured M-CSF level in plasma.
Verified Customer
Verified customer
Asked: 2014-11-27
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2014-11-27