Mouse MCP-1 PicoKine® Quick ELISA Kit

Ccl2 ELISA kit, 90-minute one-step protocol

Mouse MCP-1 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Ccl2 in cell culture supernatants and serum. Sensitivity: 6pg/ml. Cited in 2 publication(s).

Product Info Summary

SKU: FEK0568
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants and serum.
Assay Time: 90 Minutes

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Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Mouse MCP-1 PicoKine® Quick ELISA Kit

See all Ccl2 products

SKU/Catalog Number

FEK0568

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse MCP-1 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Ccl2 in cell culture supernatants and serum. Sensitivity: 6pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse MCP-1 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0568)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-R96

Sensitivity

<6 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0568 is reactive to Ccl2 in Mouse samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

FEK0568 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Ccl2

Monocyte chemotactic protein-1 (MCP-1) is a member of the small inducible gene (SIG) family. It has been shown to play a role in the recruitment of monocytes to sites of injury and infection. By analysis of a panel of somatic cell hybrids, we have localized the MCP-1 gene, designated SCYA2, to human chromosome 17. In situ hybridization confirmed this assignment and further localized the gene to 17q11.2-q21.1. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Monocyte chemoattractant protein-1 (MCP-1), regulated on activation, normal T cell expressed and secreted,and stimulation of monocytes from healthy carriers of the genotype GG with Mycobacterium tuberculosis antigens yielded higher MCP-1. The standard used in this kit is recombinant mouse MCP-1 (Q24-R96) with the molecular mass of 8.5Kda.

Kit Components

Catalog number Description Quantity
FEK0568-CAP 96-well plate precoated with anti-Mouse Ccl2 antibody 1
FEK0568-ST lyophilized recombinant Mouse Ccl2 standard 10 ng/tube
FEK0568-DA Biotinylated anti-Mouse Ccl2 detection antibody (50x) 100 ul
- Avidin-Biotin-Peroxidase Complex (30x) 400 µl
AR1106-1 Sample diluent 30 ml
AR1106-2 Antibody diluent 12 ml
- Avidin-Biotin-Peroxidase Diluent 12 ml
AR1106-5 Wash Buffer (25x) 20 ml
AR1104 Color Developing Reagent (TMB) 10 ml
AR1105 Stop Solution 10 ml
PLA-SEA Plate Sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0140.0900.1930.4060.7261.4651.8972.338

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3922439440231398
Standard deviation1.5610.7527.18212.4727.46
CV (%)4%4.8%6.9%5%5.4%6.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Ccl2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 139363434352.045.8%
Sample 22242282282192243.691.6%
Sample 339437543139439820.35.1%
*number of samples for each test n=16.

Gene/Protein Information For Ccl2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P10148

Gene ID

20296

Gene Name

Ccl2

Full Name

C-C motif chemokine 2

Weight

16.326kDa

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF Ccl2|AI323594, HC11, JE, MCA, MCAF, MCP, MCP-, MCP-1, MCP1, SMC-C, SMC-CF, Scy, Scya2, Sig, Sigje|chemokine (C-C motif) ligand 2|C-C motif chemokine 2|monocyte chemoattractant protein 1|monocyte chemotactic protein 1|platelet-derived growth factor-inducible protein JE|small-inducible cytokine A2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ccl2, check out the Ccl2 Infographic

Ccl2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ccl2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

FEK0568 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Protective Effect of Irisin on Atherosclerosis via Suppressing Oxidized Low Density Lipoprotein Induced Vascular Inflammation and Endothelial Dysfunction

Yang S, Zhang J, Wang S, Zhao X, Shi J. Oncotarget. 2017 Aug 24;8(53):91185-91198. doi: 10.18632/oncotarget.20434. eCollection 2017 Oct 31. SOCS2 overexpression alleviates diabetic nephropathy in rats by inhibiting the TLR4/NF-κB pathway

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