Mouse PD-L1/B7-H1 ELISA Kit PicoKine®

PD-L1 ELISA kit for Mouse

Mouse PD-L1/B7-H1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cd274 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 2 publication(s).

Product Info Summary

SKU: EK1450
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse PD-L1/B7-H1 ELISA Kit PicoKine®

View all PD-L1 ELISA kits

SKU/Catalog Number

EK1450

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse PD-L1/B7-H1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cd274 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse PD-L1/B7-H1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1450)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: F19-H239

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1450 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1450 is reactive to CD274 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1450 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of PD-L1

Programmed death-ligand 1 (PD-L1), also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1), is a protein that in humans is encoded by the CD274 gene. PD-L1 is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Normally the immune system reacts to foreign antigens where there is some accumulation in the lymph nodes or spleen which triggers a proliferation of antigen-specific CD8+ T cell. The formation of PD-1 receptor / PD-L1 or B7.1 receptor /PD-L1 ligand complex transmits an inhibitory signal which reduces the proliferation of these CD8+ T cells at the lymph nodes and supplementary to that PD-1 is also able to control the accumulation of foreign antigen specific T cells in the lymph nodes through apoptosis which is further mediated by a lower regulation of the gene Bcl-2.

Kit Components

Catalog Number Description Quantity
EK1450-CAP Anti-Mouse CD274 Pre-coated 96-well strip microplate 1
EK1450-ST Mouse CD274 Standard 2 vials, 10 ng/tube
EK1450-DA Mouse CD274 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0290.0980.1380.2330.4830.7291.2702.094

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect PD-L1, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 31.2 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse PD-L1/B7-H1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)7628388372304877
Standard deviation5.9621.7949.445.0429.1851.74
CV (%)6.8%7.7%5.6%7%9.6%5.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of PD-L1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 176757667733.775.1%
Sample 228329330232230014.374.7%
Sample 388376884279482144.215.3%
*number of samples for each test n=16.

Gene/Protein Information For CD274 (Source: Uniprot.Org, NCBI)

Gene Name

CD274

Full Name

Programmed cell death 1 ligand 1

Weight

33.275kDa

Superfamily

immunoglobulin superfamily

Alternative Names

B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1 CD274 B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1 CD274 molecule programmed cell death 1 ligand 1|B7 homolog 1|CD274 antigen|PDCD1 ligand 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD274, check out the CD274 Infographic

CD274 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD274: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK1450 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

FS118, a Bispecific Antibody Targeting LAG-3 and PD-L1, Enhances T-Cell Activation Resulting in Potent Antitumor Activity
Species: Mouse

Liu ZH, Zheng FF, Mao YL, Ye LF, Bian J, Lai DH, Ye YL, Dai YP. Oncol Lett. 2017 Jun;13(6):4818-4824. doi: 10.3892/ol.2017.6080. Epub 2017 Apr 24. Effects of programmed death-ligand 1 expression on OK-432 immunotherapy following transurethral rese...

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9 Customer Q&As for Mouse PD-L1/B7-H1 ELISA Kit PicoKine®

Question

Does EK1450 need to be coated?

Verified customer

Asked: 2022-04-22

Answer

No, the Mouse PD-L1/B7-H1 ELISA Kit PicoKine® (EK1450) are pre-coated.

Boster Scientific Support

Answered: 2022-04-22

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-08-29

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-08-29

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-06-06

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-06-06

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

J. Williams

Verified customer

Asked: 2020-01-14

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-01-14

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification PD-L1 using the EK1450 Mouse PD-L1 Picokine® ELISA Kit?

V. Krishna

Verified customer

Asked: 2019-09-17

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2019-09-17

Question

Is it possible to extract exosome from samples and detect PD-L1 from the exosome extraction to verify if EK1450 and EK1449 indeed detect exosomal PD-L1?

Verified customer

Asked: 2019-04-29

Answer

Our lab isn't able to perform the experiment of extracting exosome from samples and detecting PD-L1 from the exosome extraction.

Boster Scientific Support

Answered: 2019-04-30

Question

Do you know if EK1450 will react to soluble and exosomal membrane form of PD-L1? Have yoiu done any testing to confirm this?

Verified customer

Asked: 2019-04-26

Answer

Our lab technicians worked on lysates from human PBMCs and conditioned media from unstimulated human PBMCs using the Human PD-L1/B7-H1 ELISA Kit PicoKine™ (EK1449) and Mouse PD-L1/B7-H1 ELISA Kit PicoKine™ (EK1450). The concentration of PD-L1 in human PBMCs lysate is around 600pg/mL-700pg/mL while that of PD-L1 in the culture media is around 80-100pg/mL. Our lab technicians think that EK1449 and EK1450 are able to detect total PD-L1 in samples.

Boster Scientific Support

Answered: 2019-04-26

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of PD-L1 in Mouse plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2019-01-03

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the PD-L1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-01-03

Question

Q: how can I thaw whole blood sample for PD-L1 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-02-15

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test PD-L1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2018-02-15

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