Mouse PDGF-BB ELISA Kit PicoKine®

Pdgfb ELISA kit for Mouse

Mouse PDGF-BB ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse PDGF-BB in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . Cited in 1 publication(s).

Product Info Summary

SKU: EK1697
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Mouse PDGF-BB ELISA Kit PicoKine®

View all Pdgfb ELISA kits

SKU/Catalog Number

EK1697

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse PDGF-BB ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse PDGF-BB in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml .

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse PDGF-BB ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1697)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S82-T190

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1697 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1697 is reactive to Pdgfb in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1697 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Pdgfb

PDGF-BB encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

Kit Components

Catalog Number Description Quantity
EK1697-CAP Anti-Mouse Pdgfb Pre-coated 96-well strip microplate 1
EK1697-ST Mouse Pdgfb Standard 2 vials, 10 ng/tube
EK1697-DA Mouse Pdgfb Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0660.0450.1290.1980.4780.9911.8172.424

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Pdgfb, For serum, dilution ratio is 1:20 and concentration is 8216-20868 pg/ml.
For plasma, dilution ratio is 1:5 and concentration is 280-9852 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse PDGF-BB ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)14968312031507501331
Standard deviation6.748.4956.547.9543.582.5
CV (%)4.5%7.1%4.7%5.3%5.8%6.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Pdgfb in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 114917217816916710.886.5%
Sample . 268369666566367613.572.0%%
Sample 31203136913431427133582.326.1%
*number of samples for each test n=16.

Gene/Protein Information For Pdgfb (Source: Uniprot.Org, NCBI)

Gene Name

Pdgfb

Full Name

Platelet-derived growth factor subunit B

Weight

27.382kDa

Superfamily

PDGF/VEGF growth factor family

Alternative Names

PDGFBB; PDGF-BB Pdgfb|PDGF-, PDGF-2, PDGF-B, Sis, c-sis|platelet derived growth factor, B polypeptide|platelet-derived growth factor subunit B|PDGF subunit B|platelet-derived growth factor B chain|platelet-derived growth factor beta polypeptide|proto-oncogene c-Sis

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Pdgfb, check out the Pdgfb Infographic

Pdgfb infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Pdgfb: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK1697 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Liu Y,Feng M,Cai J,Li S,Dai X,Shan G,Wu S.Repurposing bortezomib for choroidal neovascularization treatment via antagonizing VEGF-A and PDGF-D mediated signaling.Exp Eye Res.2021 Jan 18;204:108446.doi:10.1016/j.exer.2021.108446.Epub ahead of print.PMID:33
Species: Mouse
EK1697 usage in article: APP:ELISA, SAMPLE:RPE TISSUE, DILUTION:NA

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Have a question?

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9 Customer Q&As for Mouse PDGF-BB ELISA Kit PicoKine®

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-05-27

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-05-27

Question

Q: how can I thaw whole blood sample for PDGFB ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-08-08

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test PDGFB for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-08-08

Question

Q: how do I analyze ELISA data? I measured PDGFB level in plasma.

S. Gonzalez

Verified customer

Asked: 2018-08-03

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-08-03

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring PDGFB using the EK1697 Mouse PDGFB Picokine® ELISA Kit?

Y. Martin

Verified customer

Asked: 2018-07-04

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2018-07-04

Question

Q: is it okay to use citrate plasma as samples in Mouse PDGFB Picokine® ELISA Kit (Catalog # EK1697)?

Verified Customer

Verified customer

Asked: 2018-06-22

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of PDGFB causing disruption of its protein structure. PDGFB may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the PDGFB ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-06-22

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

H. Wang

Verified customer

Asked: 2017-10-05

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2017-10-05

Question

Q: What is the optimal O.D. value for PDGFB ELISA kit? I performed your PDGFB ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain PDGFB even though the O.D. values are not very high?

M. Martin

Verified customer

Asked: 2017-09-19

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this PDGFB ELISA kit to have sensitivity of 10pg/ml , that means the minimum amount of PDGFB that can be declared/interpreted as positive by the above standard is 10pg/ml .

Boster Scientific Support

Answered: 2017-09-19

Question

Q: Can PDGFB ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2016-11-24

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2016-11-24

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

S. Krishna

Verified customer

Asked: 2015-07-05

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2015-07-05

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