Mouse RANK / CD265 PicoKine™ Fast ELISA Kit


SKU FEK0830
Size 96wells/kit, with removable strips.
Reactivity Mouse
Sample Type cell culture supernates and serum.
Sample Volume 100ul per well
Sensitivity <2pg/ml
Assay Range 62.5pg/ml-4000pg/ml

Introduction

Boster's Picokine™ Fast ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Overview

Product Name Mouse RANK / CD265 PicoKine™ Fast ELISA Kit
SKU/Catalog Number FEK0830
Storage & Handling Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Size 96wells/kit, with removable strips.
Description The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Mouse Rank/TNFRSF11A with <2pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates and serum. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Rank/TNFRSF11A tissue specificity: Ubiquitous expression with high levels in trabecular bone, thymus, small intestine, lung, brain and kidney. Weakly expressed in spleen and bone marrow.
Cite This Product Mouse RANK / CD265 PicoKine™ Fast ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0830)
Sample Type cell culture supernates and serum.
Sensitivity <2pg/ml
Assay Range 62.5pg/ml-4000pg/ml
Immunogen Expression system for standard: NSO; Immunogen sequence: Q30-P213
Reactivity Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Antibody Clonalities Capture Antibody | Detection Antibody:
monoclonal antibody from rat|polyclonal antibody from goat

Assay Details

Kit Components

Catalog number Description Quantity
FEK0830-CAP 96-well plate precoated with anti-Mouse Tnfrsf11a antibody 1
FEK0830-ST lyophilized recombinant Mouse Tnfrsf11a standard 10ng/tube
FEK0830-DA biotinylated anti-Mouse Tnfrsf11a antibody 130ul
AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul
AR1106-1 sample diluent buffer 30ml
AR1106-2 antibody diluent buffer 12ml
AR1106-3 ABC diluent buffer 12ml
AR1104 TMB color developing agent 10ml
AR1105 TMB stop solution 10ml
PLA-SEA Adhesive cover 4

*Why there is no wash buffer? Our Avidin-Biotin-Peroxidase Diluent contains the detergent (TWEEN) normally present in other companies' ELISA kits. This saves you the step of having to wash with the special wash buffer and achieve similar or better signal to noise ratio. The wash can use regular wash buffers (PBS, TBS etc.) commonly found in labs.

Materials Required But Not Provided

  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
  • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
  • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Typical Data Obtained from Mouse RANK / CD265 PicoKine™ Fast ELISA Kit

(TMB reaction incubate at 37°C for 15-20min)

Concentration(pg/ml)04000200040002000400020004000
O.D.0.0060.1090.2050.4320.7191.1841.6592.038

Intra/Inter Assay Precision

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)2107191032218725978
Standard deviation11.9730.1946.4415.8%542.0553.79
CV(%)5.7%4.2%4.5%7.5%5.8%5.5%

Reproducibility

Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot1 (pg/ml)Lot2 (pg/ml)Lot3 (pg/ml)Lot4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 121021619818620211.525.7%
Sample 271968570766669420.362.9%
Sample 31032104610901088106425.492.3%
*number of samples for each test n=16.

*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id O35305
Gene Name Tnfrsf11a
Protein Name Tumor necrosis factor receptor superfamily member 11A
Tissue Specificity Ubiquitous expression with high levels in trabecular bone, thymus, small intestine, lung, brain and kidney. Weakly expressed in spleen and bone marrow.
Alternative Names Tumor necrosis factor receptor superfamily member 11A;Osteoclast differentiation factor receptor;ODFR;Receptor activator of NF-KB;CD265;Tnfrsf11a;Rank;
Subcellular Localization Cell membrane ; Single-pass type I membrane protein .
Molecular Weight 66621 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. .
Research Areas Mouse

*You can search these to find other products in these research areas.
Background Receptor Activator of Nuclear Factor kappa B(RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain(residues 234-616), which contain three putative TRAF-binding domains(termed I, II, and III). RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK.

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FEK0830
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$470.00

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