Mouse Spleen Tissue Lysate

Mouse Spleen Tissue Lysate

Product Info Summary

Description: Mouse Spleen Tissue Lysate
SKU: LYSATE0017
Applications: Western blot control (20-40μg/lane)
Size: 200μg

Overview

Product Name Mouse Spleen Tissue Lysate
SKU: LYSATE0017
Description This cell lysate is prepared from mouse spleen tissue using Boster's RIPA Lysis Buffer using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Applications Western blot control (20-40μg/lane)
Quality Control 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot.
2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
3. This lysate is used to test many Boster's primary antibodies. See QC images from these antibodies for more details

Properties

Form Liquid
Size 200μg
Concentration: 2μg/μl
Formulation: The cell lysate is in SDS-PAGE sample buffer, pH 6.8, containing 50mM TRIS-HCl, 2% SDS, 100mM DTT, 10% glycerol, and 0.01% bromophenol blue.
Storage Shipped at 4˚C. Aliquot and store at -20˚C or -80˚C upon delivery. Avoid repeated freezing and thawing.

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