Mouse TFF1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Mouse TFF1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse Tff1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Product Info Summary

SKU: EZ1620
Size: For 5 plates, 96 wells each
Reactive Species: Mouse
Application: ELISA

Product Name

Mouse TFF1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all TFF1/pS2 products

SKU/Catalog Number

EZ1620

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Mouse TFF1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse Tff1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse TFF1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ1620)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q22-F87

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ1620 is reactive to Tff1 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EZ1620 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Buffers
EZ1620-CA Rabbit anti- mouse Tff1 polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) 0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol
EZ1620-DA Biotinylated goat anti- mouse Tff1 polyclonal antibody (Detection Antibody) 500 µl, 2 µg/mL (recommended dilution 1:100) 0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol
EZ1620-ST Lyophilized recombinant mouse Tff1 standard 10 ng/tube×2
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0950.220.3050.4620.781.1961.6572.031

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5224480747265781
Standard deviation3.911.9542.774.3214.5753.88
CV (%)7.5%4.9%5.3%9.2%5.5%6.9%

Gene/Protein Information For Tff1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q08423

Gene ID

21784

Gene Name

Tff1

Full Name

Trefoil factor 1

Weight

9.67kDa

Alternative Names

BCEI; BCEIbreast cancer, estrogen-inducible sequence expressed in; Breast cancer estrogen-inducible protein; breast cancer estrogen-inducible sequence; D21S21; gastrointestinal trefoil protein pS2; hP1.A; HPS2; PNR-2; Polypeptide P1.A; Protein pS2; PS2; TFF1; trefoil factor 1; trefoil factor, BCE1, human pS2 induced by estrogen from human breast cancercell line M10HP1.A Tff1|Bce, Bcei, P, PS2|trefoil factor 1|trefoil factor 1|protein pS2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tff1, check out the Tff1 Infographic

Tff1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tff1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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