Mouse TIM-3/HAVCR2 ELISA Kit PicoKine®

TIM-3 ELISA kit for Mouse

Mouse TIM-3/HAVCR2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Havcr2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1645
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Mouse TIM-3/HAVCR2 ELISA Kit PicoKine®

View all TIM-3 ELISA kits

SKU/Catalog Number

EK1645

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse TIM-3/HAVCR2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Havcr2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse TIM-3/HAVCR2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1645)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: R20-A193

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1645 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1645 is reactive to HAVCR2 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1645 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of TIM-3

Hepatitis A virus cellular receptor 2 (HAVCR2), also known as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), is a protein that in mouses is encoded by the HAVCR2 gene. The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance.

Kit Components

Catalog Number Description Quantity
EK1645-CAP Anti-Mouse HAVCR2 Pre-coated 96-well strip microplate 1
EK1645-ST Mouse HAVCR2 Standard 2 vials, 10 ng/tube
EK1645-DA Mouse HAVCR2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.2562.512525050010002000
O.D.0.0050.0710.150.3010.5751.0781.6792.030

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TIM-3, Dilution ratio of 1:1, concentration in serum and plasma is 40-60 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse TIM-3/HAVCR2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)782231009802391060
Standard deviation6%913.3841.366.8817.253
CV (%)7.3%6%4.1%8.6%7.2%5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TIM-3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 178797880780.821%
Sample 22232242262372275.592.4%
Sample 3100992798894296633.273.4%
*number of samples for each test n=16.

Gene/Protein Information For HAVCR2 (Source: Uniprot.Org, NCBI)

Gene Name

HAVCR2

Full Name

Hepatitis A virus cellular receptor 2

Weight

33.394kDa

Superfamily

immunoglobulin superfamily

Alternative Names

CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; KIM-3; SPTCL; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; TIM3; TIM-3; TIMD3; TIMD-3; TIMD3KIM-3 HAVCR2 CD366, HAVcr-2, KIM-3, SPTCL, TIM3, TIMD-3, TIMD3, Tim-3 hepatitis A virus cellular receptor 2 hepatitis A virus cellular receptor 2|T cell immunoglobulin mucin 3|T-cell immunoglobulin and mucin domain-containing protein 3|T-cell immunoglobulin mucin family member 3|T-cell immunoglobulin mucin receptor 3|T-cell membrane protein 3|kidney injury molecule-3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on HAVCR2, check out the HAVCR2 Infographic

HAVCR2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for HAVCR2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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6 Customer Q&As for Mouse TIM-3/HAVCR2 ELISA Kit PicoKine®

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-04-05

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-04-05

Question

Q: how do I analyze ELISA data? I measured TIM-3 level in plasma.

Verified Customer

Verified customer

Asked: 2019-05-15

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-05-15

Question

Q: can I use citrate plasma as samples in Mouse TIM-3 Picokine® ELISA Kit (Catalog # EK1645)?

Verified Customer

Verified customer

Asked: 2018-07-11

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of TIM-3 causing disruption of its protein structure. TIM-3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the TIM-3 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-07-11

Question

Q: how to thaw whole blood sample for TIM-3 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-02-28

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TIM-3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-02-28

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring TIM-3 using the EK1645 Mouse TIM-3 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-09-10

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2016-09-10

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2015-06-04

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2015-06-04

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