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Product Info Summary
| SKU: | LS006150 |
|---|---|
| Size: | 100 ug |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of mitochondrial ribosomal protein L23 (MRPL23), nuclear gene encoding mitochondrial protein |
Product info
Product Name
MRPL23 Human Over-expression Lysates
SKU/Catalog Number
LS006150
Size
100 ug
Description
Transient overexpression lysate of mitochondrial ribosomal protein L23 (MRPL23), nuclear gene encoding mitochondrial protein
Storage & Handling
Cite This Product
MRPL23 Human Over-expression Lysates (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006150)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay dilution & Images
Validation Images & Assay Conditions
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MRPL23 Human Over-expression Lysates NM_021134.
Western blot validation of overexpression lysate (LS006150) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with MRPL23 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Specific Publications For MRPL23 Human Over-expression Lysates (LS006150)
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