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SKU AR1156
Pack size 1 kit (for 500 tests)
Kit Components MTT labeling reagent 5ml
Formazan solubilization solution 55ml
Applications Measurement of cell proliferation in response to growth factors, cytokines and nutrients.
Measurement of cytotoxicity
To study cell activation

Overview

Product Name MTT Cell Proliferation and Cytotoxicity Assay Kit
Description Boster’s MTT Cell Proliferation Assay Kit provides a simple method for determination of cell number using standard microplate absorbance readers. Determination of cell growth rates is widely used in the testing of drug action, cytotoxic agents and screening other biologically active compounds. Several methods can be used for such determinations, but indirect approaches using fluorescent or chromogenic indicators provide the most rapid and large scale assays. The non-radioactive, colorimetric assay system using MTT was first described by Mosmann. The assay is designed for the spectrophotometric quantification of cell growth and viability without the use of radioactive isotopes. The MTT assay involves the conversion of the water soluble MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to an insoluble purple formazan crystals. The formazan crystals formed are then solubilized, and the concentration of resulting colored solution determined by optical density at 570 nm. The result is a sensitive assay with excellent linearity up to approximately 106 cells per well.
Application Measurement of cell proliferation in response to growth factors, cytokines and nutrients
Measurement of cytotoxicity
To study cell activation
Pack Size 1 kit (for 500 tests)
Kit Components MTT labeling reagent 5ml
Formazan solubilization solution 55ml

Properties

Product Type Ready to use
Form Supplied Solution
Physical State Liquid
Pack Size 1 kit (for 500 tests)
Molecular formula C18H16BrN5S
Molecular weight 414.32
CAS name/number 2H-Tetrazolium, 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-, bromide 298-93-1
Storage Upon receipt store MTT labeling reagent at -20°C. Protect from light. It is stable at -20°C for one year. Store Formazan solubilization solution at room temperature. It is stable at room temperature for one year.
Precautions FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE

Important Product Information

  • The culture conditions used to grow the cells can affect the results and must be taken into consideration when analyzing the data. The age of the cultures, number of passages and details of the growth medium can all be important factors.
  • For Formazan solubilization solution, frozen block or precipitates may form during shipment or storage, in which case the container should be warmed to +37°C and thoroughly mixed.
  • After thawing, the MTT labeling reagent may be stored protected from light at 4°C for up to 4 weeks, in which case a sterile filtration of the reagent is recommended.
  • The presence of phenol red in the final assay samples can seriously affect results. We recommend that the cells be cultured in medium free of phenol red, if possible.
  • MTT is oncogenic. Please wear gloves to operate.
  • Protocols

    Check datasheet for details:

    MTT Cell Proliferation and Cytotoxicity Assay Kit Images

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    MTT Cell Proliferation and Cytotoxicity Assay Kit
    MTT Cell Proliferation and Cytotoxicity Assay Kit
    Quantitation of U937 cells using the MTT Cell Proliferation Assay Kit. Cells in the parent culture were counted in a hemacytometer and then diluted to the indicated cell numbers in 100 µL volumes, delivered to the wells of a microplate and incubated for 4 hours to allow time for adsorption before being assayed. Absorbance measurements at 570 nm were made using a microplate reader. Each data point represents the mean value of samples in triplicate.
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    Publications

    Rapamycin Inhibits the Growth and Collagen Production of Fibroblasts Derived from Human Urethral Scar Tissue
    The effect of mast cells on the biological characteristics of prostate cancer cells
    Effect of CLIC1 gene silencing on proliferation, migration, invasion and apoptosis of human gallbladder cancer cells
    Long non-coding RNA PTENP1 interacts with miR-193a-3p to suppress cell migration and invasion through the PTEN pathway in hepatocellular carcinoma
    Compound anisodine affects the proliferation and calcium overload of hypoxia-induced rat retinal progenitor cells and brain neural stem cells via the p-ERK1/2/HIF-1?/VEGF pathway
    Differential induction of apoptosis and autophagy by pyrrolizidine alkaloid clivorine in human hepatoma Huh-7.5 cells and its toxic implication
    Single-cell pH imaging and detection for pH profiling and label-free rapid identification of cancer-cells
    Antioxidant axis Nrf2-keap1-ARE in inhibition of alcoholic liver fibrosis by IL-22
    Nur77 attenuates endothelin-1 expression via downregulation of NF-?B and p38 MAPK in A549 cells and in an ARDS rat model
    Targeting Btk with ibrutinib inhibit?gastric?carcinoma?cells?growth
    Chinese herbal formula QHF inhibits liver cancer cell invasion and migration
    Bortezomib inhibits cell proliferation in prostate cancer
    Exogenous spermine inhibits the proliferation of human pulmonary artery smooth muscle cells caused by chemically-induced hypoxia via the suppression of the ERK1/2- and PI3K/AKT-associated pathways
    Effect of ebosin on modulating interleukin-1?-induced inflammatory responses in rat fibroblast-like synoviocytes
    Paxillin regulates vascular endothelial growth factor A-induced?in vitro?angiogenesis of human umbilical vein endothelial cells
    5-FU and ixabepilone modify the microRNA expression profiles in MDA-MB-453 triple-negative breast cancer cells
    The Jagged-2/Notch-1/Hes-1 Pathway Is Involved in Intestinal Epithelium Regeneration after Intestinal Ischemia-Reperfusion Injury
    Qian YY, Li K, Liu QY, Liu ZS. Oncotarget. 2017 Nov 6;8(64):107859-107869. doi: 10.18632/oncotarget.22305. eCollection 2017 Dec 8. Long non-coding RNA PTENP1 interacts with miR-193a-3p to suppress cell migration and invasion through the PTEN pathway in hepatocellular carcinoma
    Ma Z, Yue L, Xu Z, Zeng S, Ma Y, Li Z, Li W, Wang D. Cent Eur J Immunol. 2018;43(1):1-8. doi: 10.5114/ceji.2018.74867. Epub 2018 Mar 30. The effect of mast cells on the biological characteristics of prostate cancer cells
    Chai L, Kang XJ, Sun ZZ, Zeng MF, Yu SR, Ding Y, Liang JQ, Li TT, Zhao J. Cancer Manag Res. 2018 May 3;10:989-1003. doi: 10.2147/CMAR.S163335. eCollection 2018. MiR-497-5p, miR-195-5p and miR-455-3p function as tumor suppressors by targeting hTERT in melanoma A375 cells
    He YM, Zhang ZL, Liu QY, Xiao YS, Wei L, Xi C, Nan X. J Cell Mol Med. 2018 May;22(5):2569-2579. doi: 10.1111/jcmm.13499. Epub 2018 Mar 8. Effect of CLIC1 gene silencing on proliferation, migration, invasion and apoptosis of human gallbladder cancer cells
    Yujie Jiang,1,2,* Yi Zeng,3,* Xia Huang,1,2 Yueqiu Qin,4 Weigui Luo,2 Shulin Xiang,5 Suren R. Sooranna,6 and Liao Pinhu Am J Physiol Lung Cell Mol Physiol. 2016 Dec 1; 311(6): L1023–L1035. Published online 2016 Oct 7. doi: 10.1152/ajplung.00043.2016 Nur77 attenuates endothelin-1 expression via downregulation of NF-κB and p38 MAPK in A549 cells and in an ARDS rat model

    Customer Q&As

    Q: Will using DMSO to solubilize the formazan crystals affect the cells since it is toxic at high concentrations?
    A: DMSO will not affect the cells.