*Note to educators: you are permitted to share Boster Bio's resources and PDFs on your class websites and lab websites. Please make sure to cite or link to the origin.

Designing a FACS antibody panel can get complicated, Get your flow cytometry technical guide before you start! Study an example 4-color antibody panel for immunology protocols here!
BosterBio | Antibodies, ELISA Kits, Detection Kits
October 27, 2017
Dr. Booster

Greetings Earthling,

Did you know...The mantis shrimp has SIXTEEN color-receptive cones which allow it to see a MASSIVE spectrum of color that human brains aren't even capable of processing (we only have 3 types of cones). Good thing the mantis shrimp will never get to design multicolor flow cytometry antibody panels... and good thing Boster has 6 helpful tips for humans to make multicolor panels right here! Click the shrimps for more FACS resources.

mantis shrimp

FACS antibodies multicolor panel design

1. Always try to pair the brightest fluorochromes with the weakest expressing antigen. In case the expression level of an antigen is unknown, it is advisable to use brighter fluorochromes.

2. Avoid spillover or spectral overlap between fluorochromes by spreading them as much as possible across the spectrum.

3. Avoid fluorochromes that can be excited by more than one laser e.g. APC-Cy7.

4. Always include suitable compensation controls (see our FACS resources for details) especially if points 3 and 4 above cannot be achieved.

5. It is also advisable to use FMO controls (see our FACS resources for details) to gate differently stained sub-populations more accurately.

6. Consider using online multicolor panel designers provided by FACS technology companies e.g. fluorofinder.com.

Example: A suitable 4-color panel to illustrate points 1-3. The color of the boxes correspond to the lasers that excite the respective fluorochromes.

These CD antibodies are available from Boster! Check recommended products below for validated FACS images...

example multicolor flow cytometry antibodies panel

Boster's Available Fluorophores (antibody conjugate labels):

Dyes Spectrum Graph FACS fluorescent labels table
FACS troubleshooting ebook guide download PDF

*Note to educators: you are encouraged to share BosterBio's resources and PDFs on your class and lab websites, please cite or link to origin bosterbio.com

FACS validated antibodies, click here: Shop now!

Buy Primary Antibody Get Secondary FREE
Buy Primary Antibody Get Secondary FREE
FACS Immunology Products
Anti-Human CD45 Antibody PerCP Conjugated, Flow Validated FC00555-PerCP Anti-Human CD3 Antibody FITC Conjugated, Flow Validated FC02675-FITC Anti-Human CD19 Antibody APC Conjugated, Flow Validated FC00154-APC
Anti-Human CD45 Antibody PerCP Conjugated, Flow Validated FC00555-PerCP Anti-Human CD3 Antibody FITC Conjugated, Flow Validated FC02675-FITC Anti-Human CD19 Antibody APC Conjugated, Flow Validated FC00154-APC
Anti-Human CD45 Antibody APC Conjugated, Flow Validated FC00555-APC Anti-Human CD4 Antibody APC Conjugated, Flow Validated FC00344-APC Anti-Human CD19 Antibody PE Conjugated, Flow Validated FC00154-PE
Anti-Human CD45 Antibody APC Conjugated, Flow Validated FC00555-APC Anti-Human CD4 Antibody APC Conjugated, Flow Validated FC00344-APC Anti-Human CD19 Antibody PE Conjugated, Flow Validated FC00154-PE
See all targets
Boster Guarantee

All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet. We promise to thoroughly investigate any concerns about the quality of our products; if you encounter a problem with a Boster antibody or ELISA kit, our technical support team will respond with personalized advice within 24 hours. If we can’t make your experiment work, we will refund your purchase in full or provide a replacement free of charge.

100% Satisfaction Guaranteed
Product Review
Anti-TNF Alpha Antibody (PB9246)
5 Star Rating
Review : "Under these experimental conditions...[TNF-α P28 Boster anti-CD14] demonstrated positive staining of the THP-1 cells."
Read More
Buy Primary Antibody Get Secondary FREE Rabbit Monoclonal Antibodies Custom Monoclonal Antibody Service
Buy Primary Antibody Get Secondary FREE Rabbit Monoclonal Antibodies Custom Monoclonal Antibody Service
Picokine ELISA Kits EZ Set ELISAs Multiplex Assay Services
Picokine ELISA Kits EZ Set ELISAs Multiplex Assay Services
calendar icon publication-icon star-icon antibody-icon elisa-icon
24

Years

16,000+

Publications

4.8/5

Rating on biocompare.com

12,000+

Antibodies

1000+

ELISA kits

Dr. Booster BosterBio | Antibodies, ELISA Kits, Detection Kits

Bosterbio | Antibody and ELISA Experts
3942 B Valley Ave, Pleasanton, CA, 94566
Office: 888.466.3604

www.bosterbio.com

© 2017. All rights reserved.

Follow us on Facebook Follow us on Twitter