Just last week, we received a few questions from confused researchers about weird band sizes in their western blot results.
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Predicting Western Blot Band Sizes

Predict Western Blotting band Size

First described in 1979, the technique of western blotting has since become one of the most commonly used analytical methods in life science research. Just last week, we received a few questions from confused researchers about weird band sizes in their western blot results:

1. “Why does my result show a different band size than the predicted size?”
2. “What is the expected band size? Why is it different than the observed band size?”

Click below to learn why sometimes observed bands don’t match up with the predicted bands.
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Boster's Western Blot
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