First described in 1979, the technique of western blotting has since become one of the most commonly used analytical methods in life science research. Just last week, we received a few questions from confused researchers about weird band sizes in their western blot results:
1. “Why does my result show a different band size than the predicted size?”
2. “What is the expected band size? Why is it different than the observed band size?”
Click below to learn why observed bands sometimes don’t match up with the predicted bands.
Boster takes great measures to ensure product quality and to provide our customers with comprehensive data upfront. Our antibodies are validated using WB, IHC, and flow cytometry against a panel of over 250 tissues and un-transfected cell lines to ensure high affinity and crystal-clear IHC stains. In addition, we also validate our antibodies in a quantitative fashion by testing them on known quantities of recombinant proteins so that you know what to expect (e.g. if there is 1ng, 2.5ng or 5ng of the target protein in the sample). Every product is covered by the Boster Quality Guarantee, click below to learn more.