Cellular autofluorescence is a common problem for all fluorescence-based cell detection methods. The cells’ natural fluorescence is caused by the presence of various biological structures such as collagen, elastin, NADPH, etc., which interferes with analysis by reducing signal sensitivity and resolution of fluorochromes like FITC, GFP, and Pacific Blue.
To subtract this background and prevent false positives, it is crucial to include unstained controls and set proper PMTs and gating. However, it is even more important to minimize autofluorescence as much as possible.
Click “Find out how” to learn 5 methods that help reduce autofluorescence.