p62 Dok (Phospho-Tyr398) Colorimetric Cell-Based ELISA Kit

DOK1 ELISA kit, phospho-specific, cell-based

The p62 Dok (Phospho-Tyr398) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor p62 Dok (Phospho-Tyr398) protein expression profile in cells. The kit can be used for measuring the relative amounts of p62 Dok (Phospho-Tyr398) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on p62 Dok (Phospho-Tyr398).

Product Info Summary

SKU: EKC2386
Size: 1 kit, containing 2 plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA

Product Name

p62 Dok (Phospho-Tyr398) Colorimetric Cell-Based ELISA Kit

See all DOK1 products

SKU/Catalog Number

EKC2386

Size

1 kit, containing 2 plates and all necessary reagents

*Question: How many samples can I assay/run in this kit?

Description

The p62 Dok (Phospho-Tyr398) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor p62 Dok (Phospho-Tyr398) protein expression profile in cells. The kit can be used for measuring the relative amounts of p62 Dok (Phospho-Tyr398) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on p62 Dok (Phospho-Tyr398).

Storage & Handling

4°C/6 Months

Cite This Product

p62 Dok (Phospho-Tyr398) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2386)

Clonality of Antibodies

See Datasheet for details

Sensitivity

>5000 cells

Assay Range

> 5000 Cells

Reactive Species

EKC2386 is reactive to DOK1 in Human, Mouse, Rat samples

Application Guarantee

EKC2386 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-DOK1 (Phospho-specific) Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-DOK1 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For DOK1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q99704

Gene ID

1796

Gene Name

DOK1

Full Name

Docking protein 1

Weight

52.392kDa

Superfamily

DOK family

Alternative Names

docking protein 1 (downstream of tyrosine kinase 1); docking protein 1; docking protein 1, 62kD (downstream of tyrosine kinase 1); docking protein 1, 62kDa (downstream of tyrosine kinase 1); Downstream of tyrosine kinase 1; MGC117395; MGC138860; p62(dok); P62DOK; pp62 DOK1 P62DOK, pp62 docking protein 1 docking protein 1|Downstream of tyrosine kinase 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on DOK1, check out the DOK1 Infographic

DOK1 infographic

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In this infographic you will see the following information for DOK1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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Recombinant Proteins

Boster provides a wide range of human, mouse, and rat recombinant proteins, which include cytokines, growth factors, chemokines, and many more. Our recombinant proteins have high stability, biological activity, and purity.

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Reagents

Boster offers all the reagents you need for your immunostaining and western blotting experiments. We've got everything from buffers to lysates to kits, including our popular and well-cited BCA, CCK-8, and MTT assay kits.

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