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Product Info Summary
| SKU: | LS006199 |
|---|---|
| Size: | 100 ug |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of ribosomal protein S6 kinase, 70kDa, polypeptide 2 (RPS6KB2) |
Product info
Product Name
P70 S6 Kinase beta (RPS6KB2) Human Over-expression Lysates
SKU/Catalog Number
LS006199
Size
100 ug
Description
Transient overexpression lysate of ribosomal protein S6 kinase, 70kDa, polypeptide 2 (RPS6KB2)
Storage & Handling
Cite This Product
P70 S6 Kinase beta (RPS6KB2) Human Over-expression Lysates (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006199)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay dilution & Images
Validation Images & Assay Conditions
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P70 S6 Kinase beta (RPS6KB2) Human Over-expression Lysates NM_003952.
Western blot validation of overexpression lysate (LS006199) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RPS6KB2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Specific Publications For P70 S6 Kinase beta (RPS6KB2) Human Over-expression Lysates (LS006199)
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