Phosphate Buffered Saline (PBS) Powder

Overview

Assay Principle

Phosphate Buffered Saline (PBS) powder is a dehydrated raw material reagent intended to be used in the making of prepared wash buffer by reconstitution in distilled water. The1X final solution is isotonic and pH balanced with a buffering capacity aiding in the preservation of tissue integrity and proteins functionality during IHC and other biological assay procedures. 1 packet yields tilled water into 2000ml PBS buffer.

Chemical Information

Usage and Handling

• PBS powder is safe and non-toxic, requiring no special equipment or safety procedures.

• 1 packet is ready to use for the preparation of 2000ml 1X PBS buffer solution. Dissolve the contents of a single pack into 2000ml dionized water.

Background

Phosphate Buffered Saline (PBS) is an isotonic solution containing 0.15M sodium chloride and 0.02M phosphate in the form of disodium phosphate and monosodium phosphate. PBS is used to provide a buffer system to maintain physiological pH and osmolarity, essential for tissue cultures and molecular biology experiments. PBS can be utilized as a diluting agent in preparing decimal dilutions of active reagents, as a rinsing agent for rinsing cells and cell containers, as a media for samples preparation and transportation. It is a commonly used wash buffer in immunoassay procedures, reducing non-specific binding by effectively removing unbound reagents thus preventing high background and improving the sensitivity of the assay.