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Product Info Summary
|Description:||Transient overexpression lysate of polymerase (RNA) III (DNA directed) polypeptide G (32kD)-like (POLR3GL)|
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POLR3GL (NM_032305) Human Over-expression Lysate
Transient overexpression lysate of polymerase (RNA) III (DNA directed) polypeptide G (32kD)-like (POLR3GL)
Storage & Handling
Cite This Product
POLR3GL (NM_032305) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084265)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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POLR3GL Human Over-expression Lysates NM_032305.
Western blot validation of overexpression lysate (LS084265) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with POLR3GL (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for POLR3GL (Source: Uniprot.org, NCBI)
DNA-directed RNA polymerase III subunit RPC7-like
eukaryotic RPC7 RNA polymerase subunit family
alternative RNA polymerase III subunit 32; DNA-directed RNA polymerase III subunit G-like; DNA-directed RNA polymerase III subunit RPC7-like; flj32422; FLJ34890; MGC3200; polymerase (RNA) III (DNA directed) polypeptide G (32kD) like; polymerase (RNA) III (DNA directed) polypeptide G (32kD)-like; RNA polymerase III subunit C7-like; RPC32HOM; RPC32-like protein POLR3GL RPC32HOM, SOFM, flj32422 RNA polymerase III subunit GL DNA-directed RNA polymerase III subunit RPC7-like|DNA-directed RNA polymerase III subunit G-like|RNA polymerase III 32 kDa beta subunit|RNA polymerase III subunit C7-like|RNA polymerase III subunit G like|RPC32-beta|RPC32-like protein|alternative RNA polymerase III subunit 32|polymerase (RNA) III (DNA directed) polypeptide G (32kD)-like|polymerase (RNA) III subunit G like*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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